Feasibility of microbial culture media using by-product of salt fermented kanary was investigated. Gram negative strain, Escherichia coli, and Gram positive strain, Bacillus subtilis, and bioluminescent Photobacterium phosphoreum were incubated with kanary by-product media (KB media). Compared with LB media, KB media had enough carbon source, but lacked nitrogen source and growth factor. When 0.5% of peptone as a nitrogen source and 0.3% of yeast extract as nitrogen and growth factor source were fortified in KB media, the cell population rate was similar to LB media. Also, when 0.5% of yeast extract was fortified to KB media, it showed the same result as in LB media. The price of KB media with fortification of 0.5% peptone and 0.3% yeast extract, and 0.5% of yeast extract is only 46 and 19% of that of LB media, respectively. These results showed that kanary by-product could be a good and cheaper bacterial culture media if small amount of nitrogen source and growth factor were added.