In order to determine the optimum harvest time of Acanthopanax koreanum Nakai, free sugars, organic acids, amino acids and minerals were investigated. Ash, crude fat and crude protein of 4-age`s tree of Acanthopanax koreanum were 2.98%∼3.90%, 1.93%∼2.34%, 3.52%∼4.23%, respectively. Minerals were 835.9 ㎎/100 g of K, 971.3 ㎎/100 g of Ca, 168.7 ㎎/100 g of Mg, 38.8 ㎎/100 g of Na, 18.6 ㎎/100 g of Fe, and 8.5 ㎎/100 g of Cu on average, respectively. Calcium content was increased distinguishably as late as harvest time, especially in stem and root. Glucose, fructose and sucrose content of stem were 0.71∼0.75%, 0.31∼0.03%, and 0.16∼0.21%, respectively. Organic acid was 381.0 ㎎/100 g∼504.9 ㎎/100 g, and was mainly consisted of succinic acid, citric acid and malic acid. Twelve kinds of free amino acids were detected. Total amino acids was 33.56 ㎎/100g∼48.60 ㎎/100 g, and it was the highest on samples harvested at September. Arginine, phenylalanine and glutamic acid were the main free amino acids of stems.