여러종류의 plant cellulose의 효소에 의한 가수분해에 대하여 논의하였다. Trichoderma viride로부터 얻은 cellulase에 의한 이들 물질의 가수분해는, 0.15M, pH 5.0, 아세트산 완충용액에서 50℃에서 이루어졌으며, 가수분해의 조건은 100㎎ plant cellulose에 대하여, 25㎎의 셀룰라아제 및 반응시간에 있어서 72시간이었다. 실험을 통하여 얻은 글루코오스의 최대 수율은, 반응시간 72시간에서, % saccharification으로써 옥수수대에 대하여 37.4%였다. 그리고 효모를 사용하여, 이들 글루코오스를 알코올발효시켜 이론치의 95-98%의 에틸알코올을 얻었다.

This paper deals with the enzymatic hydrolysis of different kinds of plant celluloses. The enzymatic hydrolysis of these materials by a complete cellulase from Trichoderma viride were carried out in the 0.15M acetate buffer solution at pH 5.0, 50℃, and hydrolysis conditions were 25㎎ cellulase, from Trichoderma viride for 100㎎ plant cellulose and 72 hours. The maximum yield of glucose from these experiments was 37.4% in per cent saccharification for the indian corn stem without pretreatment for 72 hours. This enzymatic hydrolyzed solution of indian corn stem was concentrated by means of reversed osmosis until 100㎎/㎖ glucose concentrations. And this glucose solution was fermented into ethanol, using the strain Saccharomyces carlsbergensis w34, giving yields of 95-98% of the theoretical value.