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SCIE SCOPUS
Differential Display Analysis of Gene Expression Induced under DCA Treatment in Rat Liver
Soon Yong Choi, Ock Jin Park
BMB Reports 29권 3호 272-275(4pages)
UCI I410-ECN-0102-2008-470-002190563
이 자료는 4페이지 이하의 자료입니다.

The expression of genes induced by Dichloroacetate (DCA) treatment was analyzed by mRNA differential display. Purified total RNAs from rat liver treated with saline or DCA (100 ㎎/100 g b.w.) were reverse transcribed by using a set of oligonucleotide primers. The PCR products were resolved on a denaturing sequencing gel. PCR band representing mRNA expressed specifically in DCA-treated liver was excised and reamplified by PCR. A 120-by c-DNA clone named IC1 was isolated and the DNA sequence of IC1 was analyzed. IC1 revealed 50% homology with 3` end of a mouse fibroblast growth factor mRNA This result indicates that DCA induces the expression of a gene which has a 50% homology with a mouse fibroblast growth factor, and expression of this gene might be involved in non genotoxic process caused by DCA.

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