The level of SecA synthesis during Sect, SecE, or Sect/SecE overproduction was examined using pulse labeling techniques and β-galactosidase assays. The level of SecA synthesis increased during the early phase of induction of SecE or Sect/SecE, whereas it declined after induction of Sect. No defect in the processing of the maltose-binding protein or OmpA was detected under these conditions, implying that the increase in SecA synthesis was not in response to a defect in protein secretion under these conditions. These results suggest that secA may be regulated by the level of certain integral Sec membrane components with which it may interact.