The compositions of gangliosides in Pinus koraiensis S. et Z. (pine nut), Juglans sinensis DODE (walnut), Armeniaca semen (apricot seed), and Prunus dulicis D.A.Webb(almond) were investigated by Folch partition, HPTLC, DEAE Sephadex A-25 column chromatography. Total polar glycolipid was extracted in aqueous phase by Folch-Suzuki method. Extracted glycolipids were separated on HPTLC glass plate in the solvent system (chloroforn : methanol : water containing 0.02% CaC1₂·2H₂0 = 55: 45 : 10, v/v/v). Glycolipids including ganglioside were visualized with resorcinol reagent or orcinol-ferric chloride-sulfuric acid reagent. The amount of total N-acetylneuraminic acid was determined using the periodate-resorcinol method. Pinus koraiensis S. et Z., Juglans sinensis DODE, Armeniaca semen, and Prunus dulicis D.A. Webb contained 0.013, 0.015, 0.050, 0.010 μ㏖ of N-acetylneuraminic acid per 10 g samples respectively. Glycolipids extracted from pine nuts 100 g and apricot seeds 100 g were analyzed on DEAE Sephadex A-25 column (acetate form, 1.3 × 14 ㎝). The gangliosides were eluted with continuous gradient of ammonium acetate in methanol (0 to 0.6 M) and our results suggest that monosialoganglioside exists in pine nuts and disialoganglioside exists in apricot nuts.