Raman spectrum of proteinase K in the crystalline state was obtained, its tentative assignment has been attempted, and the possibility of using several Raman bands for quantitative analyses has been explored. The result of Fourier deconvolution of amide I band region in the spectrum provides further support to our previous suggestion that the Fourier deconvolution can be a viable approach to the estimation of protein secondary structures by Raman spectroscopy. The surface-enhanced Raman spectrum (SERS) of subtilisin Carlsberg in the silver sol was obtained and its preliminary interpretation is given.