Multilamellar liposomes were formed spontaneously when isolated phospholipids were dispersed in glucose solution. And the effects of the amount of charged molecules in bilayer of the liposomes and the ionic strength of the medium on the forces between the bilayers of the liposomes were observed through the osmotic behavior of the liposomes using spectrophotometry. The greater the content of charged molecules, the more sensitive the osmotic behavior. The osmotic behavior of the liposomes was decreased according to the increase of the ionic strength of the glucose solution, and there was no volume change under more than 0.1 of ionic strength.