A citomase, which hydrolyze cutin ester, was expected from pine pollen Therefore pine pollen was incubated in water. And esterase activity in the water extract was measured by using p-nitrophenyl acetate (PNA) and p-nitrophenyl palmitate (PNP) as model substrates. However, the compound(S) which hydrolyze PNA and PNP was not enzyme but ninhydrin-positive compound(S). One of the compound(S) was identified as glycine.