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18.97.9.170
18.97.9.170
SCIE SCOPUS
Abstract of Papers Presented at 1981 Annual Meeting ; Isolation and Purification of New lectins from Leguminosae
See Ryun Chung , Kyung Hee Jeune Chung
BMB Reports vol. 14 iss. 2 175-176(2pages)
UCI I410-ECN-0102-2008-470-002215987
This article is 4 pages or less.

In order to find new lectins from Korean natural products, seeds or beans of 55 kinds plants belonging to 31 families were screened by using several different type of red blood cells. White kidney bean (Phaseolus vulgaris, C.,W,K,B,) and Cercis chinensis (C.C.) belonging to the Leguminosae contained strong hemagglutinating proteins. The crude lectin from W.K.B. were purified by DEAE-Sephadex A-50 column chromatography. The 0.1 M peak from this procedure demonstrated acceptable criteria, e. g. yield, optical density and hemagglutinating activity and three bands were observed in polyacrylamide disc gel electrophoresis. A further purification was achieved by hydroxyapatite column chromatography and strong hemagglutinating activity were recovered in 0. 1 M peak. It was electrophoresed and found to migrate as a single band. These suggest that the condition of purif ication for W. K. B. lectin was encourageable but did not brought agreeable satisfaction to the Cercis chinensis lectin even by these very same methods.

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