Amanita spissacea which is indiginous to Korea and Japan and Amanita vaginata which is an edible mushroom, grow wildly in Korea. However, their constituents have never been studied. These higher fungi were collected in the Gyeong Gi Province, Korea, and analyzed for their free and total amino acids, using gas chromatography and amino acid auto-analyzer. Fourteen free amino acids (alanine, glycine, valine, leucine, isoleucine, threonine, aspartic acid, methionine, phenylalanine, histidine, tyrosine, lysine, arginine, and tryptophan) were identified in both species. The contents of eight free essential amino acids in A. spissacea and a. vaginata were : threonine 7.26 and 3.15 ㎎/g, lysine 2. 85 and 2.5 ㎎/g, methionine 2.48 and 2.70 ㎎/g, phenylalanine 1.35 and 0.98 ㎎/g, isoleucine and leucine combined 1.65 and 1.05 ㎎/g, valine 1.24 and 0.68 ㎎/g, and tryptophan 0.90 and 0.93 ㎎/g, respectively. The total contents of the respective amino acids in A. spissacea and A, vaginata were: lysine 5.18 and 12.28 ㎎/g, histidine 3.00 and 5.12 ㎎/g, arginine 7.76 and 9.80㎎/g, threonine 8.88 and 8.00 ㎎/g, glutamic acid 20.10 and 34.94 ㎎/g, alanine 10.22 and 11.62 ㎎/g, valine 9.64 and 8.94 ㎎/g, glycine 8.32 and 8.30 ㎎/g, methionine 1.90 and 1. 63 ㎎/g, isoleucine 6.66 and 7.88 ㎎/g, leucine 12.74 and 14.00 ㎎/g, phenylalanine 7.00 and 7.82 ㎎/g, cysteine 0.82 and 1.26 ㎎/g, aspantic acid 16.94 and 15.28 ㎎/g, serine 9.84 and 10.34 ㎎/g and threonine 3.62 and 3.90 ㎎/g, respectively.