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면역약침의학회(구 대한면역약침학회)> 대한면역약침학회지> TA 약침의 안전성 평가 : 세균을 이용한 복귀돌연변이 시험

TA 약침의 안전성 평가 : 세균을 이용한 복귀돌연변이 시험

Safety Study of TA Pharmacopuncture: Bacterial Reverse Mutation Test

정철 ( Chul Jung ) , 황지혜 ( Ji-hye Hwang )
  • : 면역약침의학회(구 대한면역약침학회)
  • : 대한면역약침학회지 6권1호
  • : 연속간행물
  • : 2017년 12월
  • : 1-11(11pages)

DOI


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초록 보기


						
Objectives : To evaluate the safety of TA pharmacopuncture, bacterial reverse mutation test was performed using histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli (WP2uvrA (pKM101)) strains in the absence and presence of metabolic activation.
Methods : TA pharmacopuncture was prepared by extracting from a herbal mixture (Scutellariae Radix 20 g, Phellodendri Cortex 20 g, Pulsatillae Radix 20 g, Sophorae Tonkinensis Radix et Rhizoma 20 g, Aucklandiae Radix 10 g, Carthami Fructus 30 g) using a mixture solvent of purified water for injection (500 mL) and alcohol (500 mL). In order to determine the dose levels of the main study, the dose range finding study was performed. The high dose of TA pharmacopuncture was selected at 100% and it was sequentially diluted by applying a geometric ratio of 2 to produce lower dose levels of 50.0, 25.0, 12.5, and 6.25%. The treatment volume was 100 μL. As a result, the growth inhibition and precipitation of the test substance were not evident at any dose level in each strain in the absence and presence of metabolic activation. Therefore, the dose levels of the main study were selected as 100, 50.0, 25.0, 12.5, and 6.25%. Also, the negative and positive control groups were set.
Results : The mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the TA pharmacopuncture in the presence and absence of metabolic activation, showing no dose-related increase. In the positive control group, the number of revertant colonies was markedly increased by more than twice when compared to the negative control group.
Conclusions : TA pharmacopuncture did not exhibit any indications of mutagenic potential under the conditions of this study.

UCI(KEPA)

I410-ECN-0102-2018-500-004263245

간행물정보

  • : 의약학분야  > 기초한의학
  • :
  • :
  • : 연간
  • : 2287-7177
  • :
  • : 학술지
  • : 연속간행물
  • : 2012-2017
  • : 61


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발행기관 최신논문
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1TA 약침의 안전성 평가 : 세균을 이용한 복귀돌연변이 시험

저자 : 정철 ( Chul Jung ) , 황지혜 ( Ji-hye Hwang )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 1-11 (11 pages)

다운로드

(기관인증 필요)

초록보기

Objectives : To evaluate the safety of TA pharmacopuncture, bacterial reverse mutation test was performed using histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli (WP2uvrA (pKM101)) strains in the absence and presence of metabolic activation.
Methods : TA pharmacopuncture was prepared by extracting from a herbal mixture (Scutellariae Radix 20 g, Phellodendri Cortex 20 g, Pulsatillae Radix 20 g, Sophorae Tonkinensis Radix et Rhizoma 20 g, Aucklandiae Radix 10 g, Carthami Fructus 30 g) using a mixture solvent of purified water for injection (500 mL) and alcohol (500 mL). In order to determine the dose levels of the main study, the dose range finding study was performed. The high dose of TA pharmacopuncture was selected at 100% and it was sequentially diluted by applying a geometric ratio of 2 to produce lower dose levels of 50.0, 25.0, 12.5, and 6.25%. The treatment volume was 100 μL. As a result, the growth inhibition and precipitation of the test substance were not evident at any dose level in each strain in the absence and presence of metabolic activation. Therefore, the dose levels of the main study were selected as 100, 50.0, 25.0, 12.5, and 6.25%. Also, the negative and positive control groups were set.
Results : The mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the TA pharmacopuncture in the presence and absence of metabolic activation, showing no dose-related increase. In the positive control group, the number of revertant colonies was markedly increased by more than twice when compared to the negative control group.
Conclusions : TA pharmacopuncture did not exhibit any indications of mutagenic potential under the conditions of this study.

2TA 약침의 안전성 평가 : 포유류 배양세포를 이용한 염색체이상 시험

저자 : 정철 ( Chul Jung ) , 황지혜 ( Ji-hye Hwang )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 13-20 (8 pages)

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(기관인증 필요)

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Objectives : This study was designed to evaluated the potential of TA pharmacopuncture on chromosomal aberrations in Chinese Hamster Lung (CHL/IU) cells.
Methods : In order to determine the high dose level of the main study, a dose range finding study was conducted. The high dose was selected at 10.0% of undiluted TA pharmacopuncture and it was sequentially diluted to produce lower dose levels of 5.00, 2.50, 1.25, 0.625 and 0.313% by applying a geometric ratio of 2. The treatment volume was 0.3 mL. As a result, the cytotoxicity and precipitation of the test substance were not evident at any dose level in the short time treatments without and with metabolic activation and the continuous treatment without metabolic activation. Therefore, the dose levels of the main study were selected as 10.0, 5.0, and 2.5%. The treatment volume was 1.3 mL. Also, the negative and positive control groups were set.
Results : In main study, the frequency of cells with chromosome aberrations was less than 5% in the short time treatments without and with metabolic activation and the continuous treatment without metabolic activation. Also, there was not statistically significantly different when compared to the negative control group. In the positive control group, the frequency of cells with structural chromosomal aberrations was more than 10% and statistically significantly increased compared to the negative control group.
Conclusions : In conclusion, TA pharmacopuncture did not show any indication of potential to induce chromosome aberrations under the conditions of this study.

3HO약침의 약동학 연구

저자 : 정철 ( Chul Jung ) , 김학주 ( Hak-ju Kim ) , 황지혜 ( Ji-hye Hwang )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 21-28 (8 pages)

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Objectives : A pharmacokinetic study of HO-pharmacopuncture solution was performed to rats. Blood, urine and stool samples were collected and analyzed in gas chromatogram (GC).
Methods : Rats were injected intraperitoneally with HO (Carthami fructus)-pharmacopuncture solution (0.5 ml) and blood samples were collected from tail artery and urine and stool samples were acquired in individual metabolic cages.
Results : GC chromatogram analysis of HO-pharmacopuncture solution showed a total of 12 peaks. In serum samples, the largest number of peak was identified on day 1 and six peaks were observed at the same retention time as standard HO until 10 days. In stool and urine samples, peaks at 45 min were observed at the same retention time as standard HO until 10 days.
Conclusions : These results may be interpreted as most of components of HO was not accumulated in the serum but excreted as urine and stool.

4V 약침 및 한방치료로 호전된 서혜부 다한증 환자 1례

저자 : 조수호 ( Soo-ho Cho ) , 이재홍 ( Jae-hong Lee ) , 고석재 ( Seok-jae Ko ) , 박재우 ( Jae-woo Park )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 29-38 (10 pages)

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Objectives : The aim of this study is to report the effect of V Pharmacopuncture and Korean medicine treatments on primary focal hyperhidrosis at the inguinal region in a male patient.
Methods : Thirty-one years old male patient who had focal hyperhidrosis at the inguinal region was managed by V Pharmacopuncture at Zunyang [前兩. both side of Hoeeum (CV1. 會陰)] about 5 times per week, herbal medicine decoction Hyeongbangjihwang-tang 3 times per day, herbal medicine Eunhwasagan-tang 3 times per day and acupuncture treatment once a day for 25 days of hospitalization treatment.
Results and Conclusions : After 25 days of Korean medicine treatments with V Pharmacopuncture during hospitalization, hyperhidrosis and eczema at the inguinal region was markedly improved.

5TA 약침치료를 병행한 안전벨트로 발생한 흉부통증 환자 치험 3례

저자 : 정연중 ( Yeon-joong Chung ) , 이윤규 ( Yun-kyu Lee ) , 이현종 ( Hyun-jong Lee ) , 김재수 ( Jae-soo Kim )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 39-48 (10 pages)

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Objective : This study is to show the clinical effect of additional TA (Treat Anti-imflammation) Pharmacopuncture on thoracic pain.
Method : The patients were treated using TA Pharmacopuncture, and other treatments including acupunture, moxibustion and herbal medication. The effect of treatment was measured by Numeric rating scale (NRS).
Results : After treatment, NRS was gradually reduced at each case.
Conclusion : These results suggest that TA Pharmacopuncture might be a possible therapeutic option for thoracic pain caused by seat belt.

6CM 약침을 이용한 불면 환자 치험 11례

저자 : 이동규 ( Dong-gyu Lee ) , 윤정만 ( Jeong-man Yoon ) , 최예은 ( Ye-eun Choe ) , 정철 ( Chul Jung )

발행기관 : 면역약침의학회(구 대한면역약침학회) 간행물 : 대한면역약침학회지 6권 1호 발행 연도 : 2017 페이지 : pp. 49-59 (11 pages)

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Objectives : This study is to show the clinical effect of CM Immuno-Pharmacopuncture on the patients with insomnia.
Methods : Patients with insomnia were treated using CM Immuno-Pharmacopuncture. CM Immuno-Pharmacopuncture was injected into the vein 5 cc at a time. Insomnia severity index was investigated before and after treatment. Average number of treatments was about 5.27. CM Immuno-Pharmacopuncture was the only treat method during treatment.
Results : After treatment, Insomnia severity index of every patients decreased and Quality of sleep was improved. Most of patients were satisfied with CM Immuno-Pharmacopuncture.
Conclusions : CM Immuno-Pharmacopuncture may be effective in treating sleepless.

1
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KCI등재

대한본초학회지(본초분과학회지)
34권 5호 ~ 34권 5호

KCI등재

Korean Journal of Acupuncture
36권 3호 ~ 36권 3호

KCI등재

대한침구의학회지
36권 3호 ~ 36권 3호

KCI등재

대한한방부인과학회지
32권 3호 ~ 32권 3호

KCI등재

대한한의학원전학회지
32권 3호 ~ 32권 3호

KCI등재

대한본초학회지(본초분과학회지)
34권 4호 ~ 34권 4호

동국한의학연구소 논문집
10권 0호 ~ 11권 0호

KCI등재

동의신경정신과학회지
30권 2호 ~ 30권 2호

KCI등재

Korean Journal of Acupuncture
36권 2호 ~ 36권 2호

KCI등재

대한한방부인과학회지
32권 2호 ~ 32권 2호

KCI등재

대한침구의학회지
36권 2호 ~ 36권 2호

KCI등재

대한본초학회지(본초분과학회지)
34권 3호 ~ 34권 3호

KCI등재

대한한의학원전학회지
32권 2호 ~ 32권 2호

동서의학
44권 1호 ~ 44권 1호

KCI등재

Korean Journal of Acupuncture
36권 1호 ~ 36권 1호

KCI등재

대한본초학회지(본초분과학회지)
34권 2호 ~ 34권 2호

KCI등재

동의신경정신과학회지
30권 1호 ~ 30권 1호

KCI등재

대한한방부인과학회지
32권 1호 ~ 32권 1호

KCI등재

대한침구의학회지
36권 1호 ~ 36권 1호

KCI등재

대한한의학원전학회지
32권 1호 ~ 32권 1호
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