Background: Although several laser lipolysis devices have been applied in the past, their shortage of efficacy andlong treatment times are shortcomings, leading to lower utilization in lipolysis. Lasers of 1,927-nm promote greater water and fat absorption than previous wavelengths. Objectives: To evaluate the dose-dependent effects and repair processes of 1,927-nm diode laser lipolysis by analyzing histopathologic findings. Methods: Two minipigs were used, and one of the two was injected with a tumescent saline solution. Six 10 x 10 cm2 test fields were treated by a 1,927-nm diode laser with a wide range of total energy levels (4.8-7.2 W, 60-120 mJ, 40-60 Hz). Serial biopsies were performed immediately after the procedure and at 1 day, 1 week, 2weeks, 4 weeks and 3 months after laser lipolysis. The thickness of the subcutaneous fat layer was evaluated. Results: Three months after laser lipolysis, visible and palpable depressed areas on the skin surface of both minipigs were observed without clinically visible damage to the epidermis. Inflammatory cell infiltration or granulomatous reaction began after laser treatment but resolved in the follow-up period. Reduction of the thickness of the subcutaneous layer was also detected. Conclusion: The novel 1,927-nm diode laser may provide effective lipolysis with a better safety margin and faster treatment time. Further studies are warranted to evaluate efficacy and safety in human settings.