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대한산부인과학회> Obstetrics & Gynecology Science> 임신중 발생한 포진상 농가진 1예

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임신중 발생한 포진상 농가진 1예

A Cases of Impetigo Herpetiformis During Pregnancy

김동현 ( Dong Hyun Kim ) , 한승경 ( Sung Kyung Hann ) , 김세광 ( Sei Kwang Kim ) , 강승룡 ( Seung Ryong Kang ) , 이지원 ( Ji Won Yi ) , 김재욱 ( Jae Wook Kim )
  • : 대한산부인과학회
  • : Obstetrics & Gynecology Science 40권12호
  • : 연속간행물
  • : 1997년 12월
  • : 2937-2940(4pages)

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Impetigo herpetiformis is a rare disease, which traditionally has been classified among the unique dermatoses of pregnancy. It is associated· with severe maternal and fetal complications in case of misdiagnosis and delayed treatment. Diagnosis is based on the overall clinical, laboratory, and histological features. Promt and aggressive treatment is needed, and termination was effective in severe case. This pregnant woman was 32-years-old multigravida with transverse lie of fetus. At 24 weeks gestation, she developed erythematous scaly annular patchs with papules and pustules on inner thigh. The skin lesion was aggravated at 31 gestational weeks. The skin of the affected area was biopsied and the result was consisitent with impetigo herpetiformis. Treatment commenced with intravenous fluids, intravenous dexamethasone under the careful fetal well being monitoring. Cesarean section was done due to transverse lie of fetus, and a normal healthy baby was delivered, following which the patient condition improved rapidly. At one month after the delivery, the skin lesions of the patient was fully recovered.

UCI(KEPA)

I410-ECN-0102-2013-510-002158832

간행물정보

  • : 의약학분야  > 산부인과학
  • : KCI등재
  • : SCOPUS
  • : 격월
  • : 2287-8572
  • :
  • : 학술지
  • : 연속간행물
  • : 1958-2020
  • : 11006


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1토끼의 난소 과자극증후군 유발에 미치는 내인성 Angiotensin 2의 작용 및 Angiotensin-converting Enzyme Inhibitor의 치료효과

저자 : 박원일 ( Won Il Park ) , 송찬호 ( Chan Ho Song ) , 김병길 ( Pyung Kil Kim ) , 조동제 ( Dong Je Cho ) , 박기현 ( Ki Hyun Park ) , 장병철 ( Byung Chul Chang )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2647-2664 (18 pages)

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Ovarian hyperstimulation syndrome is most serious complication during ovulation induction. Although the incidence of this disease is increasing, the pathophysiology remains uncertain. Consensus is that main pathophysiology of the disease is increased vascular permeability, but the etiologic agent causing hyperpermeability is still unknown, Prostaglandin, histamine, angiotensin, some cytokines and growth factors have been suspected as etiologic agent. At present, angiotensin is mostly suspected agent of this disease. The purpose of this study is revealing etiologic role of angiotensin II in ovarian hyperstimulation and assessing the therapeutic or preventive effect of lowering angiotensin II by angiotensin converting enzyme inhibitor. After developing of ovarian hyperstimulation in rabbit, Captopril were administerd in study group. The correlations between angiotensin II and clinical parameters of severity of the disease such as ovarian volume, amount of ascites or changes of hematocrit were assessed. The correlations between angiotensin II and prostaglandin or estradiol were also assessed. The Microfil was perfused through the ovarian arteries and morphology of vascularities were revealed. The differences in ovarian volume, amount of ascites, changes of hematocrit, prostaglandin and estradiol were checked between study and control group. The parameters of severity of ovarian hyperstimulation were closely related with the level of angiotensin II. The levels of prostaglandin and estradiol were also positively correlated with the level of angiotensin II. The degree of neovascularization tended to be increased in control group, but the individual variations were existed. The parameters of severity of ovarian hyperstimulation were markedly improved in captopril group. From this data, we can conclude that angiotensin II is related with development of ovarian hyperstimulation syndrome and angiotensin converting enzyme inhibitor can prevent the development of this disease.

2역전사 중합효소연쇄반응을 이용한 태아혈액의 Interleukin-1β, Tumor Necrosis Factor-α, Transforming Growth Factor-β의 발현양상

저자 : 최석태 ( Seok Tae Choi ) , 윤보현 ( Bo Hyun Yoon ) , 신희철 ( Hee Chul Syn )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2665-2679 (15 pages)

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Background: For the successful pregnancy, the feto-maternal immunologic bidirectional interaction should be programed to accept the fetal semiallograft and assist the feto-placental growth in early pregnancy. It has been known that these signaling materials for interacting are cytokines. Up to the present, the potential roles of cytokines in normal pregnancy are, first, as a signal material of maternal immunologic recognition of fetal semiallograft, second, as a substance like growth- factor to promote the feto-placental growth and differentiation, third, as a material to alter the maternal immunity for fetal survival, fourth, as a mediator to initiate or prevent the labor. We have proposed the hypothesis that various cytokines may be expressed simultaneously, and then, may regulate the maintenance of pregnancy by interacting with each other harmonically after midpregnancy. Because the maintenance of pregnancy and the mechanism of labor depend on the total effect of various cytokines rather than the effect of one specific cytokine. Objectives: To support a potential role of the fetus itself for participating in the bidirectional cytokine regulatory network, and support our hypothesis that various cytokines may be expressed simultaneously, and then, may regulate the maintenance of pregnancy by interacting with each other harmonically after midpregnancy. Study dasign: For exploring the actual immunologic status of fetus itself in utero, we have chosen the seventeen fetal blood samples which have been taken at the cordocentesis or at the delivery without labor after midpregnancy(20th~39th weeks of gestation). After extracting the RNA from these fetal blood samples, we have tried to demonstrate the expression of interleukin-1β and tumor necrosis factor-a mRNA, and simultaneously, demonstrate the expression of transforming growth factor-β mRNA by using reverse transcriptase-po1ymerase chain reaction(rt-PCR) technique. Results: In this study we found that the expression of interleukin-1β mRNA was demonstrated from as early as 22nd weeks to term gestation, and the expression of tumor necrosis factor-α mRNA was demonstrated from as early as 2300rd weeks to term gestation, and the expression of transforming growth factor-β mRNA was demonstrated from as early as 21st weeks to term gestation. Conclusion: On the basis of these findings, first, we support the result of recent study that the fetus itself may participate in the bidirectional cytokine regulatory network after midpregnancy, second, we prove the possible hypothsis that various cytokines may be expressed simultaneously, and then, may regulate the maintenance of pregnancy by interacting with each other harmonically after mid pregnancy.

3자궁경부 종양에서 p53, Proliferating Cell Nuclear Antigen(PCNA), c-myc 및 Epidermal Growth Factor Receptor(EGFR)의 발현

저자 : 한구택 ( Ku Taek Han ) , 이형근 ( Hyung Kun Lee ) , 유순원 ( Sun Won Yoo ) , 송영훈 ( Young Hun Song ) , 최원영 ( Won Young Choi ) , 유기성 ( Ki Sung Ryu ) , 나종구 ( Jong Gu Rha ) , 송승규 ( Seung Kyu Song ) , 이헌영 ( Hun Young Lee ) , 김수평 ( Soo Pyung Kim )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2680-2691 (12 pages)

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Expression of four biologic markers known as p53, proliferating cell nuclear antigen(PCNA), c-myc, and epidermal growth factor receptor(EGFR), was studied immunohistochemically in 121 cases of cervical tissues, which included 15 cases of normal cervical tissues, 50 cases of squamous intraepithelial lesions(SIL), and 56 cases of invasive carcinomas. This study was designed to elucidate the roles of these factors in the genesis and progression of cervical neoplasia and to identify their association with clinical parameters such as cell type, tumor size, lymph node involvement, squamous cell carcinoma(SCC) antigen level, and prognosis. In additon, this study evaluates the differences of coexpression rates in normal cervical tissues, SILs, and invasive carcinomas. (1) The intensities of p53, c-myc, and EGFR expression and the rates of PCNA, c-myc, and EGFR expression in cervical SILs and invasive carcinomas were significantly higher than those in normal tissues (p<0.05 and p<0.01). But there were no significant differences between SILs and invasive carcinomas in both of intensities and rates of p53, PCNA, c-myc, and EGFR expression. (2) The intensities and rates of p53, PCNA, and  c-myc expression in the invasive carcinomas did not correlate with clinical parameters including cell types, tumor sizes, lymph node involvements, see antigen, and prognosis. But only the intensity and rate of EGFR expression were significantly higher in cases with tumor size larger than 3 cm when it was compared with cases with tumor sizes smaller than 3 cm(p<0.01), in cases with lymph node involvement when it was compared with cases without lymph node involvement(p<0.05), and in cases with SCC antigen levels more than 2.5 ng/ml when it was compared with cases with SCC antigen levels less than 2.5 ng/ml(p<0.05). (3) C-myc immunoreactivity was significantly correlated with EGFR overexpression(p<0.01). (4) The rates of simultaneous expression of four factors were decreased significantly in normal tissues and significantly increased in invasive carcinomas(p<0.01). The coexpression rate of two factors or more was 6.7% in normal tissues, 62.7% in SILs, and 70.0% in invasive carcinomas and the simultaneous expression rate of three factors or more was none in normal tissues, 34.0% in SILs, and 47.5 % in invasive carcinomas. These results show that the immunohistochemical detection of p53, PCNA, c-myc, and EGFR expression will be useful in differentiating the normal tissues and cervical neoplasia. The results of simultaneous immunohistochemical detection of these four factor expression also suggest to contribute a better understanding of the genesis of cervical carcinoma and that coexpression of more factors indicate more aggressiveness in cervical neoplasia, and show that simultaneous detection of these factors can be used in the early detection of cervical neoplasia and to predict malignant transformation of cervical lesions.

4모체혈액내 태아 유핵적혈구의 분리후PCR과 FISH에 의한 Y 염색체 검출의 임상적 유용성

저자 : 정재현 ( Jae Hyun Chung ) , 지관자 ( Kwan Ja Ji ) , 양순하 ( Soon Ha Yang ) , 오정미 ( Jung Mi Oh ) , 노정래 ( Cheong Rae Roh ) , 문영규 ( Young Kyu Moon ) , 김승욱 ( Syng Wook Kim ) , 이제호 ( Je Ho Lee )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2692-2697 (6 pages)

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Background: Fetal cells in maternal circulation offer a potential source of fetal material for non-invasive prenatal diagnosis. The presence of fetal cells in maternal blood can be confirmed by detecting Y-chromosome of male fetus by polymerase chain reaction(PCR) or fluorescence in situ hybridization(FISH). The current investigations, however, have not yet reached the accuracy for clinical application in detecting Y-chromosome by PCR or FISH because of tiny number of fetal cells in maternal blood. Therefore, if PCR and FISH were applied simultaneously on the same materials for detecting Y-chromosome, the diagnostic accuracy would be improved. Objective: The purpose of this study was to determine the clinical applicability of combined use of PCR and FISH in the detection of Y-chromosome from isolated fetal cells in maternal blood. Study Design: Peripheral blood was obtained from 43 pregnant women at 11~20 weeks `gestation. Fetal nucleated red blood cells(nRBCs) were discriminated by centrifugation under triple density gradient and by magnetic-activated cell sorter using CD 45 monoclonal antibody. The samples were resorted using CD 71 and GPA monoclonal antibodies. The PCR and FISH were performed simultaneously for the detection of Y-chromosome in each sample. Results: The diagnostic indices of PCR in the detection of Y-chromosome from fetal nRBC in maternal blood were sensitivity 70%(14/20), specificity 83%(19/23), positive predictive value 78%(14/18), and negative predictive value 76%(19/25). Those of FISH were 85%(17/20), 87%(20/23), 85%(17/20), and 87%(20/23), respectively. When PCR and FISH were concomittently applied for detecting Y-chromosome, sensitivity was 65%(13/20), specificity was 96%(22/23), positive predictive value was 93%(13/14), and negative predictive value was 76%(22/29). When PCR detected Y-chromosome and FISH did not or vice versa, it was interpreted as male. In that case, the sensitivity was 90%(18/20), the specificity was 74%(17/23), the positive predictive value was 75%(18/24), and the negative predictive value was 89%(17/19). Conclusions: When PCR and FISH were applied simultaneously, the specificity and positive predictive value in the detection of Y-chromosome from fetal cells in maternal blood were improved. However, the overall diagnostic indicies were not enough for clinical application as prenatal diagnostic technique.

5원인불명의 불임환자에서의 Transforming Growth Factor-β1, β2의 발현

저자 : 황정혜 ( Jung Hye Hwang ) , 이재억 ( Jae Auk Lee ) , 장세진 ( Se Jin Jang )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2698-2704 (7 pages)

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Objectives: The purpose of this study was to determine the differences of immunohistochemical localization of TGF-β1 and TGF-β2 in the human endometrium of fertile and infertile patients. Methods: We have studied the expression of TGF-β1 and TGF-β2 in the human endometrium of fertile and infertile patients by immunohistochemical staining method. Results: In the women with unexplained infertility, TGF-β1 and TGF-β2 were not expressed in the epithelial and stromal cells during the whole menstrual phase. But in epithelial cells of the fertile women, TGF-β1 was moderately expressed in the secretory phase and TGF-β2 was moderately expressed in the proliferative and secretory phase. In stromal cells of the fertile women, TGF-β1 and TGF-β2 were not expressed in the proliferative and secretory phase, that are similar to the results of unexplained infertility. There are significant differences in the expression of TGF-β1 and TGF-β2 between infertile and fertile patients, especially in the secretory phase of epithelial cells. Conclusions: These findings suggest that the absence of expression of TGF-β1 and TGF-β2 during the menstrual phase in women with unexplained infertility may have a negative effect on implantation and cell differentiation, and could result in ``incomplete`` embryo-maternal recognition, a possible consequence of which may be implantation failure.

6양수막을 통한 수분유출 기간 중 Li 이동에 미치는 항이뇨호르몬 및 Prolaction의 영향

저자 : 임용철 ( Yong Chul Lim ) , 최석태 ( Seok Tae Choi ) , 박노현 ( Noh Hyun Park ) , 신희철 ( Hee Chul Syn ) , 성호경 ( Ho Kyung Sung )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2705-2716 (12 pages)

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In order to investigate the effects of vasopressin and prolactin on the regulation of amniotic fluid volume and sodium concentration in pregnant rabbits, lithium transport as an index of sodium transport and amniotic fluid volume regulation through amniotic membrane was studied. Pregant rabbits were anaesthetised with pentobarbital (30mg/kg) and exposed uterus. To exclude possible contamination of residual hormone in amniotic fluid, 70 % of amniotic volume were replaced by isotonic saline. Isotonic saline (0.5ml) containing 0.5mg of congo red and 15mM of LiCl was injected into each amniotic sac. Amniotic fluid samples were taken in 30 and 60 minutes after saline injection and measured Na+, Li+ and congo red concen1rations. Only the amniotic samples which showed the decrease in congo red concentration after 30 or 60 minutes were chosen to analyse in this study. Vasopressin (25ng) or prolactin (1mg) was injected into experimental amniotic sacs and measuerd Na+, Li+, congo red and amniotic fluid volume as in control group. Then the rate of water efflux, Li-and Na-influx and efflux were ana1ysed and compared. The results were obtained as follows; 1) The efflux ratio of Na+ to water in amniotic fluid was relatively constant (about 95 %) in control, which was independent from the magnitude of water efflux rate. The efflux ratio of lithium to water, however, increased from 20 % with the increase of the magnitude of water efflux. 2) In vasopressin injected group, the water efflux rate was decreased than in control group and the efflux ratio of Na+ to water was not consistent (sometimes decreased or increased). On the other hand the efflux ratio of Li+ to water was in the same range as in control when the water efflux rate was in the range of control value, however, it profoundly increased when the water efflux rate was below 10%. 3) In prolactin treated group, the water efflux rate was very high when the initial Na+ concentration was low. The influx or efflux of Li+ was very large independently from the magnitude of water efflux rate or from initial Na+ concentration probably due to vigorous diffusion. From the above results, it is suggested that vasopressin inhibit the increase of the amniotic fluid volume during fetal urination and maintain Na+ concentration of the amniotic fluid not through the direct effect on the amniotic membrane, but thorugh the effect on fetal kidney. On the other hand, prolactin regulates Na+ concentration of the amniotic fluid very rapidly by the increase of diffusion of monovalent cations through the amniotic membrane.

7난소 종양에서 유세포측정기를 이용한 DNA 함량과 돌연변이형 p53 및 Rb 유전자 산물의 분석

저자 : 김용욱 ( Yong Wook Kim ) , 구병삼 ( Pyong Sahn Ku ) , 김인선 ( Inn Sun Kim )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2717-2727 (11 pages)

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The incidence of ovarian carcinoma has been reported to increase. Due to vague and uncharacteristic symptom and no available diagnostic procedure for early detection, diagnosis is often made in more advanced stages. Prognosis is still poor, although some progress in the efficacy of therapeutic modality has been achieved in the last decade. At the point of therapeutic view, assessment of a variety of prognostic factors is especially important. Assessment of prognostic factors which can be measured objectively and have better reproducibility should preferably be used. The cellular DNA content in the tumor cells was shown to be of prognostic significance in ovarian carcinoma. Activation of oncogenes or protooncogenes/or loss of tumor suppressor gene have been related to the development and progression of neoplasia. Many evidences have suggested that p53 acts as tumor suppressor gene in wild-type form, whereas mutant form had tumorogenic activity. This study was designed to analyze DNA content using flow cytometry in fresh ovarian tumors and to evaluate mutant p53 expression and Rb gene loss by direct fluorescent method in trypsinized, methanol-fixed, fresh cell suspensions and by immunohistochemical staining techniques in paraffin-embedded tisuue of ovarian tumor. Among 40 cases of ovarian tumors diagnosed at Korea University Hospital, 7 cases were behign, 5 cases were borderline, and 28 cases were malignant. From flow cytometric analysis, 16 cases of 28 malignant tumors(57 %) were aneuploidy which was more frequent in the advanced stages of the primary ovarian cancers than in earlier stages(p=0.002). The fraction of cells in S-phase or G2M+Sphase was lower in benign tumors compared to borderline or malignant tumors, but there was no statistical significance. From immunohistochemical study, more than 5 % of p53 positive cells were seen in 13 of 28 cases(47 %) malignant tumors and Rb gene cells were not seen in 18 of 28 cases(64%) malignant tumors, 4 of 5 cases(80 %) borderline tumors and 6 of 7 cues(86 %) benign tumors. p53 positive cells were more frequent in the advanced stages of the primary ovarian cancers(p=0.0002). Ten among 14(71.4 %) p53 positive cases were aneuploid. One among ten p53 positive and Rb gene negative cases was borderline malignancy and the other nine were malignant. These results provide that DNA ploidy pattern can be used as a prognostic factor and that mutation of p53 may play a role in ovarian oncogenesis while loss of Rb gene is not closely related to development of ovarian tumors.

8임산부의 조산경험이 다음 분만시 조산발생에 미치는 영향

저자 : 서경 ( Kyung Seo ) , 최순미 ( Soon Mi Choi ) , 조재성 ( Jae Sung Cho ) , 박용원 ( Yong Won Park ) , 이윤호 ( Yoon Ho Lee ) , 이국 ( Kook Lee )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2728-2732 (5 pages)

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The incidence of preterm delivery in subsequent pregnancy in relation to the number of previous preterm delivery was analyzed in this study. 6007 women who gave birth to two or more consecutive singleton infants at Severance Hospital, Yonsei University Medical School from January, 1961 to December, 1991 were selected as study subjects. Among the women who had preterm delivery in their first pregnancy, 20.4 % repeated preterm delivery, in their second pregnancy. 24.2 % of women who had preterm delivery in their second pregnancy, repeated on their third pregnancy, and 18.2 % in their third pregnancy repeated in their fourth pregnancy. These results showed that the incidence rates of preterm delivery were 6.8, 12.1, and 5.7 times higher, respectively, compared to that of women never experienced preterm delivery. No significant differences in preterm delivery rates in subsequent pregnancy were found according to gestational age and cause of previous preterm delivery. The diagnostic accuracy of a history of preterm delivery in predicting the repetition of preterm delivery in subsequent pregnancy were analyzed and found a sensitivity of 21.8 %, specificity of 96.8 %, positive predictive value of 20.4 %, and negative predictive value of 97 %, showing high specificity with low sensitivity. However, sensitivity improved to 44.4 % in women who repeated preterm delivery in their third pregnancy after the second pregnancy.

9폐경기 여성의 호르몬대치요법 후 혈청내의 Osteocalicin, 칼슘, Alkaline Phaosphatase 그리고 소변내의 칼슘의 변화에 관한 연구

저자 : 이천준 ( Cheon June Lee ) , 김흥열 ( Heung Yeol Kim ) , 박은동 ( Un Dong Park )

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2733-2740 (8 pages)

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To determine whether bone turnover rate is evaluated by serum osteocalcin and also to evaluate the clinical utility of serum osteocalcin, urine calcium and serum alkaline phosphatase to monitor the hormone replacement therapy(HRT) in postmenopausal women. We devided the patients into three groups. the control group was the women who were perimenopausal healthy group and the experimental groups were the postmenopansal hormon replacement therapy group and the postmenopausal nontherapy group. Serum osteocalcin, alkaline phosphatase and urine calcium were measured in all groups. The serum osteocalcin was significantly decreased in postmenopausal HRT group in comparision to postmenopausal non-HRT group(14.41±5.02 vs 16.68±6.12 ng/ml). The serum calcium was not significantly decreased in postmenopansal HRT group in comparison to postmenopausal non HRT group(8.95±1.33 vs 9.09±0.38 mg/dl). The serum alkaline phosphatase was not significantly decreased in postmenopausal HRT group in comparison to postmenopausal non-HRT group(118.73±34.18 vs 127.06±26.20 u/L). The urine calcium was significantly decreased in postmenopausal HRT group in comparison to postmenopausal non HRT group(6.9±2.12 vs 8.0±2.22 mg/dl). It is concluded that the assay of serum osteocalcin and urine calcium may be important bone metabolism indicator to druing HRT.

10세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

저자 : 이택후 ( Taek Hoo Lee ) , 김항진 ( Hang Jin Kim ) , 송건호 ( Gun Ho Song ) , 김대근 ( Dae Geun Kim ) , 전상식 ( Sang Sik Chun ) , 박윤규 ( Yoon Kyu Park ) , 서태광 ( Tae Kwang Suh ) , 전병균 ( Byeong Gyun Jeon ) , 류은경 ( Eun Kyung Ryu ) , 이은숙 ( Eun Sook Lee ) , 문진수 ( Jin Soo Moon

발행기관 : 대한산부인과학회 간행물 : Obstetrics & Gynecology Science 40권 12호 발행 연도 : 1997 페이지 : pp. 2741-2746 (6 pages)

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Immobilization of spermatozoa prior to intracytoplasmic sperm injection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization, cleavage, percentage of grade 1 embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes were inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30 μ I drop of mHTF supplemented with 10 % heat-inactivated follicular fluid(FF) at 37℃. On day 2, embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF+10 % FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to each treatment and ICSI was performed(88 head-first, 76 tail-first). The rates of normal fertilization were 81.8 % and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9 % and 68.8 %, 93.1 % and 74.1 % for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4 %(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

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PERINATOLOGY (구 대한주산의학회잡지)
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Obstetrics & Gynecology Science
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Journal of Gynecologic Oncology (JGO)
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대한폐경학회지
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KCI등재 SCOUPUS

Obstetrics & Gynecology Science
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KCI등재 SCI SCOUPUS

Journal of Gynecologic Oncology (JGO)
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PERINATOLOGY (구 대한주산의학회잡지)
31권 2호 ~ 31권 2호

KCI등재 SCI SCOUPUS

Journal of Gynecologic Oncology (JGO)
31권 3호 ~ 31권 3호

KCI등재 SCOUPUS

Obstetrics & Gynecology Science
63권 3호 ~ 63권 3호

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대한폐경학회지
26권 1호 ~ 26권 1호

KCI등재 SCI SCOUPUS

Journal of Gynecologic Oncology (JGO)
31권 2호 ~ 31권 2호

KCI등재 SCOUPUS

Obstetrics & Gynecology Science
63권 2호 ~ 63권 2호

KCI등재

PERINATOLOGY (구 대한주산의학회잡지)
31권 1호 ~ 31권 1호

KCI등재 SCOUPUS

Obstetrics & Gynecology Science
63권 1호 ~ 63권 1호

KCI등재 SCI SCOUPUS

Journal of Gynecologic Oncology (JGO)
31권 1호 ~ 31권 1호

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대한폐경학회지
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PERINATOLOGY (구 대한주산의학회잡지)
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Journal of Gynecologic Oncology (JGO)
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