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한국생약학회> Natural Product Sciences> Articles : Aqueous Extract of Schizandra chinensis Suppresses Dextran Sulfate Sodium-induced Generation of IL-8 and ROS in the Colonic Epithelial Cell Line HT-29

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Articles : Aqueous Extract of Schizandra chinensis Suppresses Dextran Sulfate Sodium-induced Generation of IL-8 and ROS in the Colonic Epithelial Cell Line HT-29

Young Mi Lee , Kang Soo Lee , Dae Ki Kim
  • : 한국생약학회
  • : Natural Product Sciences 15권4호
  • : 연속간행물
  • : 2009년 11월
  • : 185-191(7pages)
피인용수 : 38건

(자료제공: 네이버학술정보)

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Intestinal epithelial cells (IEC) play an important role in the mucosal immune system. IEC-derived mediators of inflammatory cascades play a principal role in the development of colon inflammation. The aim of this study was to investigate the inhibitory effect of aqueous extracts of Schizandra chinensis fruits (SC-Ex) on the production of inflammatory mediators by the human colonic epithelial cells. HT-29 cells were stimulated with dextran sulfate sodium in the presence or absence of SC-Ex to examine the cytoprotection and production of IL-8 and reactive oxygen species (ROS). It was shown that dextran sulfate sodium (DSS) caused the reduction of cell viability and production of IL-8 and ROS in DSS-treated HT-29 cells. We observed that the treatment of SC-Ex protected significantly cell proliferation from DSS-induced damage in dose-dependent manner. SC-Ex (10 and 100 μg/ml) also suppressed DSS-induced production of IL-8 mRNA and protein. Moreover, DSS-induced ROS production was inhibited markedly by the treatment of 100 μg/ml SC-Ex. These results suggest that SC-Ex has the protective effects on DSS-induced cell damage and the release of inflammatory mediators in the intestinal epithelial cells.

UCI(KEPA)

I410-ECN-0102-2012-510-001196714

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  • : 연속간행물
  • : 1995-2019
  • : 1102


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1Articles : Phytochemical Constituents from the Seeds of Lithospermum erythrorhizon

저자 : Jun Yeon Park , Sul Lim Lee , Saem Han , Hye Min Kim , Jeong Min Lee , Young Hee Ahn , Sook Young Lee , Sang Hyun Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 181-184 (4 pages)

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Phytochemical constituents were isolated from the seeds of Lithospermum erythrorhizon through open column chromatography and prep-HPLC. Their structures were identified as β-sitosterol (1), daucosterol (2), luteolin (3), and allantoin (4) on the basis of spectroscopic analysis. Among them, luteolin (3) was isolated for the first time from the plant.

2Articles : Aqueous Extract of Schizandra chinensis Suppresses Dextran Sulfate Sodium-induced Generation of IL-8 and ROS in the Colonic Epithelial Cell Line HT-29

저자 : Young Mi Lee , Kang Soo Lee , Dae Ki Kim

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 185-191 (7 pages)

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Intestinal epithelial cells (IEC) play an important role in the mucosal immune system. IEC-derived mediators of inflammatory cascades play a principal role in the development of colon inflammation. The aim of this study was to investigate the inhibitory effect of aqueous extracts of Schizandra chinensis fruits (SC-Ex) on the production of inflammatory mediators by the human colonic epithelial cells. HT-29 cells were stimulated with dextran sulfate sodium in the presence or absence of SC-Ex to examine the cytoprotection and production of IL-8 and reactive oxygen species (ROS). It was shown that dextran sulfate sodium (DSS) caused the reduction of cell viability and production of IL-8 and ROS in DSS-treated HT-29 cells. We observed that the treatment of SC-Ex protected significantly cell proliferation from DSS-induced damage in dose-dependent manner. SC-Ex (10 and 100 μg/ml) also suppressed DSS-induced production of IL-8 mRNA and protein. Moreover, DSS-induced ROS production was inhibited markedly by the treatment of 100 μg/ml SC-Ex. These results suggest that SC-Ex has the protective effects on DSS-induced cell damage and the release of inflammatory mediators in the intestinal epithelial cells.

3Articles : Chemical Constituents from Artemisia iwayomogi Increase the Function of Osteoblastic MC3T3-E1 Cells

저자 : Yan Ding , Chun Liang , Eun Mi Choi , Jeong Chan Ra , Young Ho Kim

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 192-197 (6 pages)

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Chemical investigation of the aerial parts of Artemisia iwayomogi has afforded five glycoside compounds. Their chemical structures were characterized by spectroscopic methods to be turpinionoside A (1), (Z)-3-hexenyl O-α-arabinopyranosyl-(1→6)-O-β-D-glucopyranoside (2), (Z)-5`-hydroxyjasmone 5`-O-β-D-glucopyranoside (3), (-)-syringaresinol-4-O-β-D-glucopyranoside (4), and methyl 3,5-di-O-caffeoyl quinate (5). All of them were isolated for the first time from Artemisia species. The effect of compounds 1-5 on the function of osteoblastic MC3T3-E1 cells was examined by checking the cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Turpinionoside A (1) significantly increased the function of osteoblastic MC3T3-E1 cells. Cell viability, ALP activity, collagen synthesis, and mineralization were increased up to 117.2% (2 μM), 110.7% (0.4 μM), 156.0% (0.4 μM), and 143.0 % (2 μM), respectively.

4Articles : Effects of α-Asarone against Global Cerebral Ischemia in Rats

저자 : Young Ock Kim , Young Soo Kwon , Seong Eun Lee , Sang Won Lee , Hee Jae Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 198-202 (5 pages)

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Based on the use of Acorus gramineus SOLAND (AG) for the treatment of stroke in traditional Korean medicine, the present study was carried out to evaluate neuroprotective effects of α-asarone after transient global cerebral ischemia using rat 4-vessel occlusion (4VO) model in rats. α-Asarone (5 mg/kg) administered intraperitoneally significantly protected CA1 neurons against 10 min transient forebrain ischemia as demonstrated by measuring the density of neuronal cells stained with Cresyl violet. α-Asarone significantly reduced hippocampal neuronal cell death by 85.2% where as its isolated single compounds from AG compared with a vehicle-treated group.

5Articles : Minor Phenolic Constituents of the Anemarrhenae Rhizoma

저자 : Ui Joung Youn , Ye Seul Lee , Ha Na Jeong , Joo Won Nam , Yoo Jin Lee , Young Min Son , Eun Sook Hwang , Eun Kyoung Seo

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 203-207 (5 pages)

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A homoisoflavanone, 7,4`-dihydroxyhomoisoflavanone (1) and a flavanone, (2S)-7,4`-dihydroxy-5-methoxyflavanone (2), were isolated from the rhizomes of Anemarrhena asphodeloides, together with 4,4`-dihydroxychalcon (3), 2`-O-methylphlorethin (4), 1,3-bis-di-p-hydroxyphenyl-4-penten-1-one (5), and 2,4`-dihydroxy-4-methoxybenzophenone (6) on the basis of spectroscopic and physicochemical analyses including 1D-and 2D-NMR techniques as well as by comparison of their data with the published values. Compounds 1-4 were isolated for the first time from this plant source. Among isolates, compound 2 exhibited moderate inhibitory effect on the differentiation of pre-adipocyte 3T3-L1 cells.

6Articles : Quantitative Analysis of Fustin and Sulfuretin in the Inner and Outer Heartwoods and Stem Bark of Rhus verniciflua

저자 : Min Young Kim , Ill Min Chung , Deog Cheon Choi , Hee Juhn Park

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 208-212 (5 pages)

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The heartwood of Rhus verniciflua Stokes (Anacardiaceae) is widely used for a medicinal plant to treat diabetes mellitus and lingering intoxication in the folkloric society of Korea, while the stem bark has been traditionally used to treat menstrual disorder and helminthiasis. We previously reported that a flavonoid, fustin, isolated from the heartwood of R. verniciflua is effective in Alzheimer`s disease and rheumatoid arthritis. To explore the possibility to produce more flavonoid-rich fraction from this plant, the MeOH extracts from the plant parts of stem bark, outer heartwood, and inner heartwood were quantitatively analyzed by HPLC. Phenolic or flavonoid compounds (chlorogenic acid, caffeic acid, p-coumaric acid, sulfuretin, fustin, fisetin, luteolin and astragalin) were detectable in the HPLC chromatogram. The orange-colored inner heartwood was found to contain the highest levels of fustin (16.96 mg/g) and sulfuretin (2.22 mg/g). Moreover, the inner heartwood accumulated fustin and sulfuretin at least 4-fold higher level as compared to the stem bark and outer heartwood. The levels of total phenolic compounds positively correlated with the extents of antioxidant properties. Therefore, the inner heartwood of R. verniciflua could be used to increase fustin concentration of the extract which is capable of treating Alzheimer`s disease and rheumatoid arthritis.

7Articles : Methanol Extract of Longanae Arillus Regulates Sleep Architecture and EEG Power Spectra in Restraint-Stressed Rats

저자 : Yuan Ma , Jae Soon Eun , Kwang Seung Lee , Eun Sil Lee , Chung Soo Kim , Bang Yeon Hwang , Ki Wan Oh

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 213-221 (9 pages)

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Longanae Arillus (the rind of fruits of Dimocarpus longan) has been consumed for the treatment of insomnia and anxiety in Asia. To provide further scientific basis to traditional uses of this fruit on insomnia, we evaluated the effects of methanol extract of Longanae Arillus (MELA) on the alteration of sleep architecture and electroencephalogram (EEG) power spectra in acutely and chronically restraint-stressed rats. Following postsurgical recovery, Polygraphic signs of sleep-wake activities were recorded for 24 h after MELA administration in rats. Rats in the acute stress and chronic stress were administered with MELA for 10 days. On the 8th, 9th and 10th day of MELA administration, the rats were stressed for 3 h once per day. On the 10th day and 1 h after MELA administration, the rats were stressed once for 22 h in the chronic stress group. Acute and chronic stress induced alternations in cortex EEG recordings during non-rapid eye movement (NREM), rapid eye movement (REM) sleep and wakefulness. MELA shortened the total and REM sleep and increased the wakefulness in night time recording without changing daytime recordings. Chronic stress increased wakefulness and REM sleep, decreased total and NREM sleep in the daytime recording, and increased REM and decreased NREM sleep without changing total sleep and wakefulness in night time recording. These findings suggest that MELA ameliorated the alterations in REM and NREM sleep of acutely and chronically stressed rats via modulation of cortical α-, θ- and δ- wave activity.

8Articles : Bamboo Culm Extract Downregulated Activation of NKT- and B-cells and Production of IL-6 in Pristane-Induced Lupus Mice

저자 : Byeong Suk Chae , Byung Hyun Park

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 222-228 (7 pages)

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Lupus is characterized by immunoregulatory abnormalities between T- and B-cells leading to autoantibody production and multiorgan injuries. We investigated whether bamboo culm extract (BC) ameliorates aberrant activation of T cells and B cells and attenuate production of IL-6 in pristane-induced lupus mice. Lupus was induced by i.p. a single injection of 0.5 ml of pristane in female BALB/c mice, which, later about 4 months, were used as a lupus model. The pristane-induced lupus mice and healthy mice were injected i.p. with BC 5 μl/kg or PBS once a day for 3 weeks. These results demonstrated that BC significantly decreased levels of serum and BAL IL-6 and production of IL-6 by macrophages with/without LPS, and downregulated expression of NKT cell and CD86+ CD45R/B220+, but not CD80+CD45R/B220+ and CD69+CD4+ in the splenocytes in pristine-induced lupus mice. Moreover, BC greatly increased Con A-stimulated production of IL-6, IL-10 and IFN-γ by splenocytes obtained from pristane-induced lupus mice. Therefore, our findings suggest that BC may ameliorate lupus pathogenesis in pristane-induced lupus mice via downregulation of aberrant activation of NKT cells and B cells and inhibition of production of IL-6.

9Articles : In Vitro Anti-Inflammatory Effects of the Essential Oil of Artemisia iwayomogi and Its Main Component, Vulgarone B

저자 : Eun Young Chung , Seung Won Shin

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 229-233 (5 pages)

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The essential oil fraction was obtained from the leaves and flowers of Artemisia iwayomogi (Compositae) by steam distillation, and its main component, vulgarone B, was isolated by column chromatography. RAW 264.7 cells were used to investigate the anti-inflammatory properties of A. iwayomogi and vulgarone B. Cell viability was determined by MTT assay after treatment with various dilutions of the compounds. In addition, several assays were used to determine the effects of A. iwayomogi essential oil components on immune stimulation. Nitric oxide production in cells activated with lipopolysaccharide (LPS) was evaluated by reaction with Griess reagent. Both vulgarone B and the essential oil fraction of A. iwayomogi inhibited the production of nitric oxide. The effects on various cytokines released from the cells were also measured using ELISA. The production of prostaglandin E2 was significantly decreased by treatment with A. iwayomogi oils. LPS-induced IL-1β and IL-6 production were also decreased in a dose-dependent manner, but no significant effect on TNF-α was observed at the concentrations tested. Finally, Western blot analysis revealed that A. iwayomogi oils reduced the levels of COX-2 and iNOS.

10Articles : Phytochemical Constituents of Bistorta manshuriensis

저자 : Sang Wook Chang , Ki Hyun Kim , Il Kyun Lee , Sang Un Choi , Shi Yong Ryu , Kang Ro Lee

발행기관 : 한국생약학회 간행물 : Natural Product Sciences 15권 4호 발행 연도 : 2009 페이지 : pp. 234-240 (7 pages)

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Phytochemical investigation of the MeOH extract of the aerial parts of Bistorta manshuriensis resulted in the isolation of two cerebrosides, two lactams, six phenolic compounds and seven flavonoids. Their chemical structures were characterized by spectroscopic methods to be pinelloside (1), soyacerebroside I (2), pterolactam (3), 5-hydroxypyrrolidine-2-one (4), vanillic acid (5), caffeic acid methyl ester (6), protocatechuic acid (7), caffeic acid (8), 3,5-di-O-caffeoyl quinic acid methyl ester (9), chlorogenic acid methyl ester (10), avicularin (11), afzelin (12), quercetin (13), isoorientin (14), quercetin 3-O-β-D-glucoside (15), quercitrin (16), and luteolin (17). The isolated compounds (1-4, 7, 12, 14) were isolated for the first time from this plant source and the compounds 1-4, 9 and 10 were first reported from the genus Bistorta. Compound 17 exhibited moderate cytotoxicity and compound 6 exhibited weak cytotoxicity against four human cancer cell lines in vitro using an SRB bioassay.

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