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대한면역학회> 대한면역학회지> 자외선 조사가 Listeria monocytogenes 의 감염에 대한 감수성과 싸이토카인 생성에 미치는 영향

자외선 조사가 Listeria monocytogenes 의 감염에 대한 감수성과 싸이토카인 생성에 미치는 영향

The Effect of Ultraviolet lrradiation on Susceptibility of Listeria monocytogenes lnfection and the Production of Cytokines

유욱(Wook Lew) , 장수경(Soo Kyoung Chang) , 김길영(Kir Young Kim) , 이봉기(Bong Ki Lee)
  • : 대한면역학회
  • : 대한면역학회지 21권4호
  • : 연속간행물
  • : 1999년
  • : 353-360(8pages)

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Although the effect of ultraviolet (UV) irradiation on immune response was reported to supress cellular immune response, the exact mechanism was not elucidated. As recent development in cytokine research progress, it is well-known that immune response is regulated by cytokines and especially cellular immune response is induced by interferon (IFN)-r and interleukin (IL)-12 which is mainly produced from lymphocytes and macrophages respectively. Therefore our purpose was to elucidate the UV effect on cellular immune response and its mechanism. We have investigated the changes of host resistance by injection of Listeria monocytogenes which is an intracellular parasite after UVB irradiation in C57BL/6 mice which is known to have relatively strong cellular immune response. In addition we also have investigated the changes in the production of IFN-r from lymphocytes and the production of tumor necrosis factor (TNF)-a and IL-12 from macrophages in mice by UVB irradiation. The increase of mouse spleen index and susceptibility of iisteria monocytogenes infection was correlated with the decreased production of IFN-r, TNF-a and IL-12, which was known to induce the suppression of cellular imrnune response. 
Korean J. Immunol. 21, 4: 353~360, 1999

UCI(KEPA)

I410-ECN-0102-2009-510-004783638

간행물정보

  • : 의약학분야  > 미생물학
  • :
  • :
  • : 계간
  • : 1015-6453
  • :
  • : 학술지
  • : 연속간행물
  • : 1983-2000
  • : 866


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1재조합 IL - 15 에 의한 마우스 비장 단핵구의 면역활성에 미치는 영향

저자 : 한인숙(Insook Han) , 박종욱(Jong Wook Park)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 297-302 (6 pages)

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After the synthesis of IL-15 cDNA from the total RNA of mouse spleen, it was inserted into the prokaryotic expression vector, pRseta, and eukaryotic expression vector, pcDNA3.0, respectively. Subsequently, the insertion of gene and open reading frame were confirmed by sequencing of each plasmid, respectively. Using pRseta- IL-15 plasmid, the recombinant IL-15 protein was induced by IPTG under BL21 (DE 3) host cells and recombinant IL-15 was expressed at 14.5 KDa with time. Then, IL- 15 was separated by His-tag affinity chromatography and analyzed by SDS-PAGE to yield soluble IL-15 at 14.5 KDa as monomer and 29.0 KDa as dimer. In order to inspect the function and contribution of IL-15, the in vitro experiment was established using mononuclear cells separated from the mouse spleen. After 48h exposure of PHA to mouse splenocyte and 24h treatment with recombinant IL-15, the effects of cytokine inductions inspected against IL-2, IL-6, IL-10, IL-12, IFN-r, and GM-CSF. The results showed that comparing with the control, IL-6 increased, IL-2, IL-12 and IFN-r increased and similar, and GM-CSF decreased. In addition, the direct injection of pcDNA3.0-IL-15 plasmid into mice gave the similar results to in vitro studies. Namely, IL-6 and IL-12 increased, and IL-2, IFN-r and GM-CSF were similar or decreased. IL-10 was not induced in in vitro and in vivo experiments. These results suggested that the IL-15 induce the splenocyte activation and can be an important factor in proliferation and fuction recovery of weakened T-cell. 
Korean J. Immunol. 21, 4: 297-302, 1999

2CD44v6 의 항체분절 생산과 그들의 특이성 비교

저자 : 한인숙(Insook Han) , 전석길(Seok Kil Zeon) , 박관규(Kwan Kyu Park)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 303-309 (7 pages)

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CD44v6 was known as tumor marker for tumor progression and metastasis in various kinds of carcinomas. The CD44v6 monoclonal antibody was produced by cell cultures or mouse ascite fluids using CD44v6 hybridoma cells, and its immunogloburin G (IgG) was purified by Protein A column. Using immobilized ficin and cysteine, the antibody fragment Fab was produced and purified by Protein A. Four CD44v6 scFv molecules were produced from the recombinant DNA and phage antibody technology and prurified by His-tag affinity chromatography. In order to inspect the function and specificity of each antibody molecule, western-blotting and ELISA against CD44v5-6 recombinant proteins and irnmunodetection in human ovarian carcinomas were estabilished. The results showed that immunodiagnosis did not distinguish the types of antibody fragments, but western-blotting and ELISA results did show some difference of their specificities and biological properties. These studies will contribute as a model study for the immunodiagnosis and therapy using the IgG, Fab and scFv of CD44v6 antibody to obtain the early detection of tumor progression and metastasis using immunoscintigraphy. Korean J. Immunol. 21, 4: 303-309, 1999

3Connective Tissue Growth Factor 에 대한 Antisense Ollgodeoxynucleotides 를 이용한 성체 창상의 반흔 조절

저자 : 최병민(Byung Min Choi) , 오기수(Gi Su Oh) , 신용섭(Yong Sub Shin) , 백상기(Sang Gi Paik) , 정현택(Hun Taeg Chung)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 311-317 (7 pages)

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Adult wounds heal with scar formation, whereas fetal wounds heal without scarring and with a lesser inflammatory and cytokine response. Recently connective tissue growth factor (CTGF) is known to play an important role in wound healing. We reasoned that a strategy employing antisense oligodeoxynucleotides (ODN) complementary to CTGF mRNA by topical application of the ODN on the skin wound. Phosphorothioation of ODN to retard their degradation. When antisense CTGF ODN were applied on the wound site, there was a marked reduction of scarring compared with a control wound site. This effect of antisense CTGF ODN on scar forrnation was associated with decreased expression of the CTGF gene. However, sense CTGF ODN had no effect on the expression of the CTGF gene. In addition, control wounds healed with excessive fibrosis compared with the antisense-treated wounds. In conclusion, our results indicate that antisense CTGF ODN could be used for ameliorating scar formation during wound healing. Korean J. Immunol. 21, 4: 311~317, 1999

4능동성 전신성 아나필락시스의 경구 관용

저자 : 신영미(Young Mi Shin) , 최일환(II Hwan Choi) , 이헌구(Hern Ku Lee)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 319-325 (7 pages)

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We have investigated whether oral administration of ovalbumin (OVA) could prevent active systemic anaphylaxis to the antigen. Oral tolerance was induced by a single feecfing with 40 mg OVA before, but not after, sensitization characterized by diminished OVA-specific IgE and IgG responses. Feeding 15 mg OVA suppressed anaphylaxis and antibody responses to a lesser extent. Spleen cells from tolerant donors were incapable of transferring the tolerance to naive recipients. Pretreatment of cyclophosphamide (100 mg/kg) 2 days before OVA feeding did not restore the tolerance. Furthermore, in vitro cell mixing studies showed that the proliferation of spleen cells from OVA- sensitized donors was not inhibited by the addition of spleen cells from tolerant donors, arguing against the role of suppressor cells. Anergy was demonstrated by the ability to reverse the tolerant state after culturing tolerant cells in the presence of IL-2. These findings indicate that only a high-dose (40 mg) feeding OVA was found to be effective in inducing tolerance in this experimental system, and demonstrate anergy as the mechanism underlying oral tolerance to systemic anaphylaxis. 
Korean J. Immunol. 21, 4: 319-325, 1999

5Suppression PCR 을 이용한 공제 보합결합법에 의한 유도성 비만세포 유전자의 동정

저자 : 조정제(Jeong Je Cho) , 하윤문(Youn Mun Ha)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 327-334 (8 pages)

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The mast cell is an essential effector cell in allergic inflammation through its capacity to respond to IgE dependent activation. Mast cells also participate in the modulation of physiologic processes, but the role of mast cell in these processes is still unclear. Recently, the number of structurally defined chernoattractants for leukocytes has greatly increased, owing to largely to the identification of the chemokine superfamily. In this study we examined the pattern of expression of chemokines and their receptors in HMC-1 after treatment with PMA/A23187 and/or LPS using RT-PCR and ELISA. Messenger RNA of IL-8, the representative CXC chemokine, was induced after PMA/ A23187 treatment. All of the CC chemokines tested, except eotaxin, were induced after PMA/A23187 treatment. CCR1, CXCR2, CXCR3 and CXCR4 were expressed in all test groups regardless of activation. CCR3 was expressed only at 3 hours of activation. CCR2, CCR5 and CXCR1 were not expressed in mast cell line. Production of most of chemokine proteins was not detected in resting state and increased significantly after 3 hours of activation with PMA/A23187. The effect of LPS treatment was negligible. MCP-1 protein was always produced without activation and accurnulated in a time-dependent rnanner. These data suggest that the expression of mRNA and protein of chemokines and chemokine receptors are regulated transcriptionally and translationally. Human mast cell may respond to various stimuli by producing chemokines and their receptors to regulate their function and may act autonomously or through other inflammatory cell that they recruited. Korean J. Immunol. 21, 4: 335  342, 1999

6사람 비만 세포주 ( HMC - 1 ) 에서 케모카인 및 그 수용체의 유도 및 발현

저자 : 이희문(Hee Moon Lee) , 조정제(Jeong Je Cho) , 윤주천(Joo Chun Yoon) , 하윤문(Youn Mun Ha)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 335-342 (8 pages)

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The mast cell is an essential effector cell in allergic inflammation through its capacity to respond to IgE dependent activation. Mast cells also participate in the modulation of physiologic processes, but the role of mast cell in these processes is still unclear. Recently, the number of structurally defined chernoattractants for leukocytes has greatly increased, owing to largely to the identification of the chemokine superfamily. In this study we examined the pattern of expression of chemokines and their receptors in HMC-1 after treatment with PMA/A23187 and/or LPS using RT-PCR and ELISA. Messenger RNA of IL-8, the representative CXC chemokine, was induced after PMA/ A23187 treatment. All of the CC chemokines tested, except eotaxin, were induced after PMA/A23187 treatment. CCR1, CXCR2, CXCR3 and CXCR4 were expressed in all test groups regardless of activation. CCR3 was expressed only at 3 hours of activation. CCR2, CCR5 and CXCR1 were not expressed in mast cell line. Production of most of chemokine proteins was not detected in resting state and increased significantly after 3 hours of activation with PMA/A23187. The effect of LPS treatment was negligible. MCP-1 protein was always produced without activation and accurnulated in a time-dependent rnanner. These data suggest that the expression of mRNA and protein of chemokines and chemokine receptors are regulated transcriptionally and translationally. Human mast cell may respond to various stimuli by producing chemokines and their receptors to regulate their function and may act autonomously or through other inflammatory cell that they recruited. Korean J. Immunol. 21, 4: 335  342, 1999

7Lipoarabinomannan 자극 THP - 1 세포에서 IFN - r 에 의한 Chemokine Mig 와 IP10 mRNA 의 유도 항진

저자 : 이황호 , 이황호(Hwang Ho Lee)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 343-351 (9 pages)

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Lipoarabinomannans (LAM) is believed as a potential virulence factor of Mycobacterium tuberculosis. LAM exhibits marked differences in biological activities depending on the types, arabinofuranosyl-terminated LAM (AraLAM) derived from a rapidly growing Mycobacterium sp. and heavily mannosylated LAM (ManLAM) derived from the Erdman strain. Collaboration between macrophages and T cells, especially macrophage activation by gamma interferon (IFN-r) and chemoattraction of T cells at the very inflammatory foci would be essential in defence against M. tubercu/osis. Chemokines Mig and IP-10 are inducible by IFN-r from macrophages and have been shown to act in vitro as T cell chemoattractants. However, little is known of LAMs capacity to induce chemokines Mig and IP-10 in macrophages. In this experiment, Mig and IP10 mRNA was expressed in the delayed-type hypersensitivity (DTH) against BCG in BCG-immune mice. In some experiments, both Mig and IP-10 mRNA was evidently induced with different time courses in THP-1 cells stimulated with whole live M. tubercu/osis H37Rv (Erdman). To investigate whether Mig and IP-10 genes are differentially induced depending on the type of LAM, PCR amplification was used to detect mRNA of Mig and IP-10 from the THP-1 human monocytic cells stimulated with LAM. AraLAM, but not ManLAM, induced weakly Mig and IP-10 mRNA in the THP-1 cells. The induction of Mig and IP-10 was dependent upon the dose of AraLAM and exhibited different time courses. The mRNA for Mig and IP-10 was induced within 2 hr and 4 hr from the initiation of treatrnent and has disappeared by 8 hr and 24 hr under the experimental conditions used in this study, respectively. IFN-y at 100 U/ml, but not at 10 U/ml, was itself a good stimulus of both Mig and IP- 10 expression, and synergized with either AraLAM or ManLAM for induction of both Mig and IP-10. The expression patterns of MCP-3 were somewhat similar to those of Mig and IP10 in all of the experiments. These data indicate that IFN-r may contribute to effective macrophage function if macrophages are not fully affected by ManLAM, and chemokines Mig and IP-10 may a role in recruitment of T cells at inflammatory foci of tuberculosis. Korean J. Immunol. 21, 4: 343-351, 1999

8자외선 조사가 Listeria monocytogenes 의 감염에 대한 감수성과 싸이토카인 생성에 미치는 영향

저자 : 유욱(Wook Lew) , 장수경(Soo Kyoung Chang) , 김길영(Kir Young Kim) , 이봉기(Bong Ki Lee)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 353-360 (8 pages)

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Although the effect of ultraviolet (UV) irradiation on immune response was reported to supress cellular immune response, the exact mechanism was not elucidated. As recent development in cytokine research progress, it is well-known that immune response is regulated by cytokines and especially cellular immune response is induced by interferon (IFN)-r and interleukin (IL)-12 which is mainly produced from lymphocytes and macrophages respectively. Therefore our purpose was to elucidate the UV effect on cellular immune response and its mechanism. We have investigated the changes of host resistance by injection of Listeria monocytogenes which is an intracellular parasite after UVB irradiation in C57BL/6 mice which is known to have relatively strong cellular immune response. In addition we also have investigated the changes in the production of IFN-r from lymphocytes and the production of tumor necrosis factor (TNF)-a and IL-12 from macrophages in mice by UVB irradiation. The increase of mouse spleen index and susceptibility of iisteria monocytogenes infection was correlated with the decreased production of IFN-r, TNF-a and IL-12, which was known to induce the suppression of cellular imrnune response. 
Korean J. Immunol. 21, 4: 353~360, 1999

9일차 배양 사람 성상교세포의 Fas 매개 세포죽음에 대한 Cycloheximide 와 Dexamethasone 의 영향

저자 : 최철희(Chulhee Choi) , 최인홍(In Hong Choi) , 허균(Kyoon Huh)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 361-368 (8 pages)

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Astrocytes are major glial cells in central nervous system (CNS) and are known to express death receptors or ligands that can induce apoptosis of astrocytes or other brain cells. We have previously confirmed that cultured human astrocytes express fas and fas ligand and their expression may be regulated by various cytokines found in CNS. Because fas can rnediate cell death known as apoptosis, we investigated fas-mediated cell death in cultured human astrocytes and evaluated factors that may influence the fas-mediated apoptosis in astrocytes. Pretreatment of interferon-r and TNF-a increased cell death in astrocytes. Cell death induced by fas ligation was confirmed as apoptosis by phosphatidylserine translocation in cell membrane. Cycloheximide, protein synthesis inhibitor, potentiated fas-mediated cell death. However, buthionine sulfoxine did not potentiate fas-mediated apoptosis. Dexamethasone blocked cell death in dose-dependent and time-dependent manners. These findings collectively show that fas expressed on cultured human fetal astrocytes can induce apoptotic cell death after pretreatment of interferon-r and/or TNF-a. Therefore, the fas-fas ligand system in CNS may regulate the glial degeneration and may participate the neuronal loss in certain conditions. Furthermore, fas-mediated apoptosis of astrocytes can be potentiated by protein synthesis inhibitors and can be blocked by dexamethasone. 
Korean J. Immunol. 21, 4: 361-368, 1999

10Jurkat T 세포주 활성화에서 Vav 와 T 세포 수용체 Chain 간의 상호 작용에 관한 분석 ( Analysis of lnteraction between Vav 와 T 세포 수용체 Chain 간의 상호 작용에 관한 분석 )

저자 : 채욱진(Wookjin Chae) , 한진환(Jinhwan Han) , 김상원(Sangwon Kim) , 송영섭(Youngsup Song) , 조경민(Kyungmin Cho) , 이명철(Myungchull Rhee) , 이상규(Sang Kyou Lee)

발행기관 : 대한면역학회 간행물 : 대한면역학회지 21권 4호 발행 연도 : 1999 페이지 : pp. 369-375 (7 pages)

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When T cells are encountered with MHC (Major Histocompatibility Complex): peptide complex, they can be activated via T cell receptor complex. Among T cell receptor complex,  chains have three ITAMs (Immunoreceptor Tyrosine based Activation Motifs) in their cytoplasmic tail for triggering the diverse intracellular downstream signaling cascades. To analyze the importance of each ITAM, we made stable Jurkat transfectants expressing high level of CD8- chimeric mo~lecule and its mutant forms where the tyrosine residues in each ITAM were changed into phenylalanine on their surface. Mutant transfectants showed distinct pattern of tyrosine phosphorylation upon activation including changes in tyrosine phosphorylation of GNEF (Guanine Nucleotide Exchange Factor) Vav. Among three ITAMs, A ITAM was proved to be critical role for association with Vav for activation of Jurkat stable transfectants. Mutant transfectants mutated at A ITAM (A1 and A1A2) showed remarkable reduction in phosphorylation and association of Vav with  chain and other important signaling molecules including ZAP-70 and SLP-76 compared to the other transfectants. This finding suggests that Vav can deliver the downstream activation signaling via association with A ITAM directly or indirectly. 
Korean J. Immunol. 21, 4: 369-375, 1999

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