목적: 중합효소연쇄반응법과 in situ hybridization법을 결합시킨 정위중합효소연쇄반응법 (polymerase chain reaction in situ hybridization, PCR/ISH)법을 이용하여 자궁경부병변에서 인유두종바이러스의 핵산(HPV DNA)을 규명하고, 같은 조직병변에서 in situ hybridization(ISH)법을 이용한 결과와 비교하기 위해 본 연구를 시행하였다. 연구방법 및 대상: 1998년 5월부터 7월까지 자궁경부질확대경 조준하 자궁경부생검상 자궁경부상피내종양 및 침윤성 자궁경부암으로 진단받은 15예 및 32예를 이용하여 PCR/ISH법을 적용시켜 인유두종바이러스 핵산을 규명하고, 같은 조직병변을 ISH법을 적용시켜 결과를 비교하였다. 결과: 자궁경부상피내종양에서 ISH를 사용하여 26%, PCR/ISH법으로는 80%의 양성율을 보였고, 침윤성 자궁경부암에서는 각각 40%,84%의 결과를 얻었으며, 모든 예에서 ISH양성이면 PCR/ISH양성이었고 ISH양성이면서 PCR/ISH음성인 예는 하나도 없었다. 결론: PCR/ISH법은 ISH처럼 조직학적 병변의 형태를 그대로 유지하면서, ISH보다는 훨씬 높은 검출율을 보여 효과적인 HPV DNA 검출기법이 될 것으로 사료된다.

Objective: The aim of this study was to identify human papilloma virus(HPV) infection in cervical lesions by using polymerase chain reaction in situ hybridization(PCR-ISH) and to compare the results of PCR-ISH with conventional in situ hybridization(ISH) Methods: Forty-seven randomly chosen neutral-buffered formalhyde-fixed cervical biopsies in which cervical intraepithelial neoplasia and invasive cervical cancer had been diagnosed were tested for HPV DNA by PCR-ISH and ISH. The method of PCR-ISH includes deparaffinization of specimens, PCR amplification of DNA, in situ hybridization and detection of amplified products. Results: The positivity rate of ISH was 36% for all biopsies and 26%, 40% for CIN and invasive cancer respectively. By using PCR-ISH the positivity was 80%, 84% respectively. All PCR-ISH positive cases were ISH positive and no ISH positive was PCR-ISH negative. Conclusion: In our study HPV DNA, undetectable by standard in situ hybridization, was detectable by PCR in situ hybridization. Our study shows that PCR in situ hybridization is highly sensitive method for detection of HPV in cervical neoplastic specimens with morphological evidence.