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한국동물실험대체법학회> 동물실험대체법학회지

동물실험대체법학회지 update

Journal of Alternatives to Animal Experiments

  • : 한국동물실험대체법학회
  • : 의약학분야  >  기타(의약학)
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수록정보
수록범위 : 1권1호(2007)~13권1호(2019) |수록논문 수 : 96
동물실험대체법학회지
13권1호(2019년 12월) 수록논문
최근 권호 논문
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KCI후보

1인공피부모델을 이용한 LTE 대역 전자파의 피부 영향 연구

저자 : 김규리 ( Kyu-ri Kim ) , 임경민 ( Kyung-min Lim )

발행기관 : 한국동물실험대체법학회 간행물 : 동물실험대체법학회지 13권 1호 발행 연도 : 2019 페이지 : pp. 5-10 (6 pages)

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Skin is located on the outermost layer of body, which poses it to easy and frequent exposure to toxic chemicals, physical stimuli and environmental contaminants. Depending on the stimuli, outcomes of skin toxicity may differ, which include commonly observed, and evident skin reactions like skin burn, irritation, sensitization, phototoxicity, or photosensitivity. Less common reactions like pigmentary disorders, urticaria, and fibrosis can also occur. Effects of sunlight on the skin have been well established and with this, radiations with longer wavelength are gathering interest for their effects on the skin health. In this study, we investigated the effects of electromagnetic field (EMF) of LTE bandwidth, 1.742 GHz, SAR 6-8 W/kg on the skin by using an artificial human skin model. The results indicated that EMF exposure does not have much effect on epidermal cytotoxicity or obvious pigmentation in the skin, but it has a slight effect on skin epidermal integrity and melanocyte activation. The mechanism and the final outcome of EMF exposure should be further studied through more scientific studies and various molecular biochemical studies.

KCI후보

2Spectrophotometric Direct Peptide Reactivity Assay (Spectro-DPRA)를 위한 펩타이드의 보관조건에 따른 품질 변성 연구

저자 : 박교현 ( Kyo-hyun Park ) , 서정아 ( Jung-ah Seo ) , 김배환 ( Bae-hwan Kim )

발행기관 : 한국동물실험대체법학회 간행물 : 동물실험대체법학회지 13권 1호 발행 연도 : 2019 페이지 : pp. 11-16 (6 pages)

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The Spectrophotometric direct peptide reactivity assay (Spectro-DPRA) is now in process for the development and validation of evaluation on skin-sensitizing chemicals. The cysteine peptides (Ac-RFAACAACOOH) and lysine peptides (Ac-RFAAKAA-COOH) is used for Spectro-DPRA.
The objective of this study is to confirm the stability of cysteine and lysine peptides which are used for Spectro-DPRA through the storage conditions. We analyzed the stability of peptide using HPLC according to the conditions, such as storage temperature and storage period.
The peptide powders of cysteine and lysine were stable on storage -80℃, for 6 and 12 month. And 10 mM stock peptides of cysteine and lysine were also stable on storage -70℃, for 1, 3 and 6 month. But 10 mM stock peptides of cysteine were denatured and not stable on storage -20℃, for 1 month. Taken together, peptides powder for Spectro-DPRA should be stored under -80℃, less than 12 month. And, 10 mM stock peptides should be also stored under -70℃, less than 6 month.

KCI후보

3호흡기 3D 조직모델(SoluAirwayTM)의 품질과 독성평가에 대한 국내 확립 연구

저자 : 정미호 ( Mi-ho Jeong ) , 김설영 ( Seol-yeong Kim ) , 김준우 ( Jun-woo Kim ) , 방인재 ( In-jae Bang ) , 김가은 ( Ga-eun Kim ) , 김혜수 ( Hye-soo Kim ) , 이수현 ( Su-hyon Lee ) , 김하룡 ( Ha-ryong Kim )

발행기관 : 한국동물실험대체법학회 간행물 : 동물실험대체법학회지 13권 1호 발행 연도 : 2019 페이지 : pp. 17-23 (7 pages)

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The concern of health and safety is growing due to increasing exposure of inhalable chemicals. However, there is no appropriate alternative testing method for assessing the hazard caused by inhalation exposure. The aim of this study is to establish respiratory 3-dimentional (3D) tissue model which can be applied for assessing the toxicity testing in Korea.
First of all, respiratory 3D tissue model, SoluAirway™ was reconstituted by using human nasal mucosa. For evaluating its stability, the viability, Trans-Epithelial Electric Resistance (TEER), and histology was analyzed after delivered to 3 areas in Korea. Then, bleomycin (BLM) and polyhexamethylene guanidine phosphate (PHMG) which were known as toxic materials in pulmonary system were treated to various 3D tissue models to demonstrate applicability in evaluating chemical toxicity. The cytotoxicity and secretion of inflammatory cytokines was acquired with MTT and ELISA, respectively.
The cell viability, TEER and integrity of the 3D tissue model which quality was controlled between products, had no significantly different values after delivery. The cytotoxicity of BLM and PHMG showed dose-dependently. Furthermore, the secretion of IL-8 was shown at the highest concentration of BLM and PHMG-p in SoluAirway™, not in EpiAirway™. When applied to KeraSkin™ which was a skin model, the secretion of IL-8 was declined. Taken together, these data showed that SoluAirway™ could be used as toxicity testing model for inhalable substances in Korea.

KCI후보

4치약 및 함유 성분의 구강 점막 자극 평가를 위한 새로운 in Vitro 시험법 적용

저자 : 조선아 ( Sun-a Cho ) , 김유진 ( Yoo-jin Kim ) , 김찬호 ( Chan-ho Kim ) , 김영소 ( Young-so Kim ) , 안수선 ( Su-sun An )

발행기관 : 한국동물실험대체법학회 간행물 : 동물실험대체법학회지 13권 1호 발행 연도 : 2019 페이지 : pp. 25-38 (14 pages)

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Oral hygiene products, such as toothpastes, are essential for personal hygiene. However, these products contain substances that cause mucosal irritation but are essential to maintain functionality. Until now, there is no in vitro animal test to evaluate oral mucosal irritation for oral hygiene products and its ingredients. Moreover, oral hygiene products are classified as cosmetics in EU, thus the irritation test for these products and its ingredients are prohibited. In Korea, oral products are classified as quasi-drugs now, but there is a high possibility to change to cosmetics according to global trends. So we needed to set up the in vitro test system to evaluate oral mucosal irritation and we studied the oral mucosal irritation for toothpaste and its ingredients by agar diffusion test. In the evaluation of ingredients, the use of surfactant, tooth whitening ingredient and some functional ingredients affect the increase of cytotoxicity. In addition, when diluted to 25%, the toothpastes showed the broad range of irritation and it was appropriate to judge the level of irritation. From the results, we can set the evaluating method for toothpaste and its ingredients and internal criteria for the mucosal irritation at same time.

KCI후보

5분광광도법을 이용한 간단한 in Chemico 피부감작성 시험법의 개발

저자 : 차동호 ( Dong-ho Cha ) , 네팔마헤시 ( Mahesh Raj Nepal ) , 김건호 ( Geon-ho Kim ) , 강미정 ( Mi-jeong Kang ) , 정태천 ( Tae-cheon Jeong )

발행기관 : 한국동물실험대체법학회 간행물 : 동물실험대체법학회지 13권 1호 발행 연도 : 2019 페이지 : pp. 39-47 (9 pages)

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For the key event No. 1 in skin sensitization, a hapten should react with endogenous proteins for the initiation of immune reaction. Although Direct Peptide Reactivity Assay (DPRA) has been developed as an alternative method for key event 1, artificially synthesized peptides and HPLC should be employed in performance, which are somewhat inconvenient. In the present study, a simple spectrophotometrical method to identify skin sensitizers in chemico was developed by using cysteamine, an endogenous substance that contains amino and thiol groups. To quantitate remaining cysteamine following the reaction with skin sensitizers, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) was selected for the derivatization. The conditions optimized included: incubation time of cysteamine with test substances, molar ratios of cysteamine to test substances, stability of derivatized products of test substances with optimal concentration and incubation time of DTNB, and spectrophotometrical characteristics of the derivatized product. With the optimized conditions, 11 skin sensitizers and non-sensitizers were tested to know whether the method is feasible or not. Although further optimization is required, it would be a useful screening tool for determining skin sensitization potential of small molecules, because the present method employs simple endogenous cysteamine as an acceptor for sensitizers with a spectrophotometric detection system,.

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1연안해역에서 석유오염물질의 세균학적 분해에 관한 연구

(2006)홍길동 외 1명심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동41회 피인용

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