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한국원예학회> HEB (구 한국원예학회지)

HEB (구 한국원예학회지)

HORTICULTURAL, ENVIRONMENT AND BIOTECHNOLOGY

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수록정보
수록범위 : 1권0호(1965)~48권3호(2007) |수록논문 수 : 2,783
HEB (구 한국원예학회지)
48권3호(2007년 06월) 수록논문
최근 권호 논문
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KCI등재

1Production of Doubled Haploid Onions (Allium cepa) and Evaluation of Their Field Performance

저자 : Sung Gil Kim , Kil Sun Yoo , Leonard M. Pike

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 143-147 (5 pages)

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Doubled haploid lines in onion (Allium cepa) are homozygous for all loci and can be used as valuable materials for inheritance studies of complex traits and pure line production for F1 hybrid breeding. In this study, doubled haploid lines and their F1 hybrids were produced and their field performance was compared with commercial open-pollinated cultivars. Six doubled haploid lines were produced from haploid culture. Four F1 hybrids were produced by crossing doubled haploid lines with sterile breeding lines. Doubled haploid lines, F1 hybrids, and five commercial open-pollinated cultivars were grown at two locations. After harvesting the bulbs, equatorial diameter, polar diameter, shape index, weight, soluble solid content, and pungency were measured. Doubled haploid lines and F1 hybrids generally showed higher uniformity as compared with commercial cultivars for all traits. Hybrid vigor, a good response to favorable growing conditions, and high stability were observed in F1 hybrids. Overall, it was demonstrated that doubled haploid lines were more uniform than open-pollinated cultivars and could be utilized as parental lines for F1 hybrid production with high uniformity.

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2The Use of Molecular Markers to Certify Clubroot Resistant Cultivars of Chinese Cabbage

저자 : Zhong Yun Piao , Su Ryun Choi , Young Mi Lee , Hong Gi Kim , Yong Pyo Lim

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 148-154 (7 pages)

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Chinese cabbage cultivated in most area of Korea is subjected to the most serious clubroot disease caused by Plasmodiophora brassicae Wor. A number of clubroot resistant (CR) cultivars were released or in development. We have previously tagged CR locus (CRb) within 3 cM flanked by two co-dominant markers (TCR01 and TCR05) and one dominant marker (TCR09). Thirty-seven CR cultivars and ten non-CR cultivars were collected to study the utility of these DNA markers. In addition, markers (BRIVIS096, BRMS088, and OPC11-2S) linked to Crrl, Crr2, and Crr3, respectively, were evaluated. Of the surveyed CR cultivars, 18 carried marker fragment TCR01200 and TCR05279 linked to CRb in coupling. Evaluation of these cultivars to race 4 of clubroot revealed that all of them conferred resistance. Other CR cultivars absent of TCR01200 and TCR05279 were either resistant or susceptible. Only OPC11-2S linked to Crr3 can explain the two CR cultivars showing susceptible, while others cannot. Analysis of European Clubroot Differential set 01 to 04 indicate that marker fragment TCR05279 with the CRb locus, possibly also TCR01200, was introgressed from CR turnip into partial CR cultivars of Chinese cabbage. The usefulness of these markers in marker-assisted selection was also discussed.

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3Effects of Short-Term CO2 Treatment on Physiological Characteristics and Quality of Fresh-Cut Cabbage

저자 : Ki Wung Kwak , Jong Nam Park , Jang Hun Kyong , Cheon Soon Jeong

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 155-158 (4 pages)

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This study was conducted to investigate the effects of high CO2 short-term treatment on the physiological characteristics and the quality changes in fresh-cut cabbage. Fresh-cut cabbages were placed in barrier film and sealed up with air (control) or 30, 60, and 90% CO2 balanced with N2 for 6 h at room temperature. Fresh-cut cabbages were separately packaged with a wrap, and then stored at 13℃ for 3 days. Respiration and production of ethylene increased immediately after CO2 treatment. The blackening and yellowing appeared on the cut surface of cabbages and red cabbage, respectively, as the storage period was extended. However, the discoloration of cabbages was delayed with high CO2 treatment. CO2 treatment of 90% maintained effectively ascorbic acid contents after 3 days storage at 13℃. Weight loss was somewhat high in fresh-cut cabbages treated with CO2, but was found insignificant.

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4Effects of Aminoethoxyvinylglycine on Preharvest Drop, Fruit Color, and Quality of "Tsugaru" Apples

저자 : In Kyu Kang , Jae Kyun Byun , Hun Joong Kweon , Mok Jong Kim , Soon Il Kwon , Moo Yong Park , Doug Hoon Lee , Cheol Choi , Doug Geun Choi

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 159-164 (6 pages)

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This study was carried out to investigate the effects of aminoethoxyvinylglycine (AVG) on harvest date extension and storability promotion in ``Tsugaru`` apples. AVG at 75 mg·L-1 was applied once 4, 3, 2, and 1 weeks prior to the predicted harvest date, and twice 2 and 1 weeks prior to and after 1 week the predicted harvest date. The predicted harvest date of ``Tsugaru`` apples was esteemed approximately August 20. AVG decreased preharvest drop greatly by 14-30%, especially when they were harvested about 1 month later (September 20) than normal harvest date, and increased fruit weight by 35.8%. Two times application decreased preharvest drop by 14-16%, while one time application by 22-30%. During this period, flesh firmness and acidity decreased, and the fruit red color (Hunter value a) increased. Respiration decreased slightly, ethylene production greatly decreased, and flesh firmness and acidity were higher in AVG-sprayed fruits than in control fruits. When AVG-sprayed fruits were stored at 0±1℃ for 60 days, they maintained higher fruit quality than control fruits. Cell wall hydrolases activities decreased in AVG-sprayed fruits compared to control fruits during cold storage. Especially, α- and β-galactosidase activities greatly decreased in AVG-sprayed fruits. Therefore, the results indicated that AVG treatment will be an effective method to prevent preharvest drop and to improve fruit quality in ``Tsugaru`` apples.

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5Several Factors Affecting Embryogenic Culture Maintenance and Shoot Regeneration in "Miyagawa Wase" Satsuma Mandarin (Citrus unshiu)

저자 : Seong Beom Jin , Kwan Jeong Song , Key Zung Riu

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 165-170 (6 pages)

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To establish an efficient embryogenic culture and regeneration system in ``Miyagawa Wase`` satsuma mandarin (Citrus unshiu Marc.), several factors affecting embryogenic culture maintenance and plant regeneration were investigated. The highest multiplication of embryogenic culture was obtained on culture media supplemented with 5% sucrose, regardless of medium type. When fractioned by Percoll gradient, the callus collected from the layer of 40% showed higher embryogenesis capacity than the others. Agar concentration and carbohydrate source in the medium also affected somatic embryogenesis and the highest embryogenesis was obtained at 1.4% agar and 7% lactose. The highest cotyledon development from somatic embryos was observed by supplement of 0.7 mg·L-1 adenine into the medium. The normal shoots could be regenerated from the section culture of abnormal cotyledons on the medium containing 1-naphthaleneacetic acid (NAA) and N6-benzyladenine (BA). The most effective concentrations of NAA and BA were 0.01 and 0.1 mg·L-1, respectively. The normal plants were obtained through micro-grafting onto rootstocks of trifoliate orange and acclimatization in a plastic greenhouse.

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6Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA

저자 : Jin Ung Yun , Hee Beom Yang , Yong Hwan Jung , Su Hyun Yun , Kwang Sik Kim , Chan Shick Kim , Kwan Jeong Song

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 171-175 (5 pages)

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The study aimed at evaluating an efficiency of cross breeding in ``Miyagawa Wase`` and ``Okitsu Wase`` satsuma mandarin and ``Shiranuhi`` mandarin through investigating the seed and seedlings formation, the frequency and position of zygotic seedlings, and the influence of pollen parents using randomly amplified polymorphic DNA (RAPD). Seedlings were in vitro cultured with seeds obtained from four crosses of ``Miyagawa Wase`` (Citrus unshiu)×``Ponkan`` (C. reticulata), ``Okitsu Wase`` (C. unshiu)×``Swingle Citrumelo`` (C. paradisi×Poncirus trifoliate), ``Shiranuhi`` [(C. unshiu×C. sinensis)×C. reticulata]×``Ponkan`` (C. reticulata), and ``Shiranuhi`` [(C. unshiu×C. sinensis)×C. reticulata]×``Swingle Citrumelo`` (C. paradisi×P trifoliate). RAPD analysis was conducted with five primers selected from screening 77 primers based on DNA amplification of parent plants. Seed formation including total seeds and full sized seeds per fruit between ``Miyagawa Wase`` and ``Okitsu Wase`` was not different statistically. However, seed formation of ``Shiranuhi`` mandarin was significantly affected by pollen genotype. Average seedlings number per seed was not different effectively. The frequency and positioning patterns of zygotic seedling were different in each cultivar. The results suggest that seed formation, polyembryony, and frequency and positioning of zygotic seedling in some citrus having polyembryony might be affected by seed and pollen parents.

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7Characterization of the Corylopsis coreana Using Molecular Markers

저자 : Mark S. Roh , Ae Kyung Lee , Ik Young Choi , Jae Yeong Kim , Young Hee Joung , Sun Ha Lee , Jeung Keun Suh

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 176-187 (12 pages)

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This research was initiated to study the inter-specific relationships and intra-specific variations in the Corylopsis glabrescens complex that includes C. coreana Uyeki, C. glabrescens Franch. et Say var. glabrescens, and C. glabrescens var. gotoana (Makino) T. Yamanaka (collectively called the C. glabrescens complex) by examining accessions collected from natural habitats, various botanical gardens, and arboreta. Randomly amplified polymorphic DNA (RAPD), single nucleotide polymorphisms in the internal transcribed spacer (ITS) region of ribosomal DNA (nrDNA-ITS region) specific for C. glabrescens Franch. et Say., phytochrome C gene, and NADH dehydrogenase subunit 1 gene analyses were performed. The results of RAPD markers showed that accessions were clustered into three major groups; the first group consisting of C. coreana, C. glabrescens var. glabrescens, C. glabrescens var. gotoana, and C. paucflora Siebold & Zucc.; the second group of C. sinensis Hemsi., C. veitchiana Bean, C. glanduhfera Hemsl., and C. spicata Siebold & Zucc, and the third group of C. sinensis Hemsi. var. calvescens Rehder & E. H. Wilson with some exceptions. C. coreana, C. glabrescens var. glabrescens, and C. glabrescens var. gotoana grouped into one major cluster. Although nrDNA-ITS region cannot be effectively used for identification of the C. glabrescens complex at the level of species, C. sinensis var. calvescens clustered separately from C. sinensis var. sinensis. C. coreana accessions collected from Korea were separated from different posterior probability populations by STRUCTURE program from C. glabrescens var. glabrescens and also from var. gotoana collected from Japan. This support the establishment of C. coreana as a separate species based on this study.

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8Physiological Response and Purification Efficiency of Indoor Plants Exposed to Ozone

저자 : Seung Il Jung , Min Ji Kim , Ki Cheol Son , Pan Gi Kim , Jae Cheon Lee , Stanley J. Kays

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 188-196 (9 pages)

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The effects of ozone on indoor foliage plants and their ozone removal efficiency were studied. Cissus rhombifolia Vahl, Hedera helix L., Spathiphyllum wallisii Regel, and Syngonium podophyllum Schott ``Albo-Virens`` were exposed to 120 nL·L-1 ozone for 2, 4, or 8 h·day-1 for 25 days in walk-in growth chambers. Changes in stomatal conductance, transpiration rate, and intercellular CO2 concentration were monitored throughout the light and dark periods (13/11 h) and photosynthesis and respiration during the light and dark, respectively. The injury caused by ozone and the plant`s potential for and extent of recovery varied among species. In C. rhombifolia and S. wallisii plants exposed to ozone for 8 h·day-1, photosynthetic rate, stomatal conductance, and ozone uptake decreased throughout the 25-day treatment period. The photosynthetic rate, stomatal conductance, and ozone uptake rate of H. helix and S. podophyllum initially decreased (through day 7), but then began to recover though never reaching pre-exposure levels. Cumulative ozone uptake rate was highest in S. wallisii. Uptake rate at day 25 decreased as the daily exposure time increased. Ozone uptake by C. rhombifolia and S. aureus was significantly reduced when exposed to ozone for 8 h·day-1 compared with the 2 h-day-1 exposure. Ozone tolerant species had higher uptake rates. The results indicate that while plants remove environmental ozone at a significant rate, they sustain injury during the process. The extent of the injury and the plant`s ability to adapt vary with species, length of daily exposure, and duration of exposure. Photosynthetic rate and stomatal conductance provide a means in which the adverse effect of ozone on plants can be monitored.

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9Particle Bombardment-Mediated Transformation and Regeneration of Marigold (Tagetes erecta)

저자 : Hyung Jun Kim , Seung Beom Hong , Sook Yon Mo , Doo Hwan Kim

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 197-200 (4 pages)

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We examined the possibility of transforming and obtaining transgenic marigold (Tagetes ercta L.) plants using the particle bombardment process. Conditions for optimal delivery of DNA to cotyledonous explants were established for three cultivars of marigold plant. The size of microcarriers, distance to target, and He pressure significantly affected transformation and regeneration efficiency. A total of approximately 1,000 cotyledonous explants for each cultivar were bombarded with DNA-coated particles and placed on selection medium containing 50 mg·L-1 kanamycin. The regeneration rates before bombardment were between 17 and 38% in 1/2 MPM medium supplemented with 5 or 7 mg·L-1 benzylaminopurine. However, the regeneration rate was reduced to 4% at most after bombardment.

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10Identification of Atractylodes japonica and A. macrocephala by AFLP and SCAR Markers

저자 : Man Kyu Huh , Kyong Hwan Bang , Joo Soo Choi

발행기관 : 한국원예학회 간행물 : HEB (구 한국원예학회지) 48권 3호 발행 연도 : 2007 페이지 : pp. 201-205 (5 pages)

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Backchul, Atractylodes japonica or A. macrocephala, is a very important Chinese medicinal herb plant in Korea. This study was carried out to develop a method that could be used to discriminate between A. japonica and A. macrocephala based on molecular markers. Amplified fragment length polymorphism (AFLP) analysis was carried out to develop sequence characterized amplified region (SCAR) markers. Initially, 176 reliable AFLP bands were produced with sizes ranging from 100 to 500 bp. A total of 74 (42%) of these bands were polymorphic. The phenotypic frequency of each band was calculated and used in estimating genetic diversity (H) within populations. The mean H of A. japonica (0.211) was higher than that (0.146) of A. macrocephala. One AFLP marker, AjAF-1 contained 168 bp nucleotide sequences and was specific to A. japonica. The AmAF-1 marker contained 138 bp nucleotides and was specific to A. macrocephala. The SCAR markers derived from these two AFLP markers enabled a rapid discrimination between A. japonica and A. macrocephala, as well as between Korean and Chinese herbal medicines obtained from Korean herbal markets.

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