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한국동물위생학회(구 한국가축위생학회)> 한국가축위생학회지 (KOJVS)

한국가축위생학회지 (KOJVS) update

Korean Journal of Veterinary Service

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수록정보
수록범위 : 13권1호(1990)~43권4호(2020) |수록논문 수 : 1,139
한국가축위생학회지 (KOJVS)
43권4호(2020년 12월) 수록논문
최근 권호 논문
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1소 결핵 진단을 위한 인터페론감마 검사 키트의 성능 비교 평가

저자 : 홍이곤 ( Leegon Hong ) , 최우재 ( Woojae Choi ) , 노영혜 ( Younghye Ro ) , 안선민 ( Sunmin Ahn ) , 김은경 ( Eunkyung Kim ) , 최은희 ( Eunhee Choe ) , 김단일 ( Danil Kim )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 201-209 (9 pages)

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In Korea, bovine tuberculosis (bTB) is a representative zoonotic disease that causes considerable economic loss. In determining the positive bTB, the ELISA method for examining the amount of interferon- gamma (IFN-γ) is included in Korea's diagnostic standard method. Recently, commercially available BIONOTE TB-Feron ELISA Plus (TB-Feron Plus) that detects IFN-γ has been introduced. However, since the scientific basis for the performance is limited, we evaluated performance by comparing it with the results of another IFN-γ ELISA assay kit (BOVIGAM  ) certified by Office International des Epizooties. In our research, 42 positive blood samples preliminarily tested with a tuberculin skin test and/or BOVIGAM  and 54 negative blood samples collected from three bTB free farms were subjected to IFN-γ assay using the TB-Feron Plus and the BOVIGAM  , respectively. The result shows that the sensitivity, specificity and accuracy were 81.0% (34/42), 100% (54/54), 91.7% (88/96) in TB-Feron Plus kit and 78.6% (33/42), 100% (54/54), 90.6% (87/96) in BOVIGAM  kit, respectively. Moreover, the overall accordance percentage of the two kits was 99.0% (95/96) and there was almost perfect agreement between two assays (Kappa=0.977, P<0.0001). Furthermore, additional studies confirmed that elevated lymphocyte numbers in blood did not interfere with the results of the TB-Feron Plus kit. And, delayed time from sampling to culture decreased the optical density (OD) value. Therefore, we concluded that the TBFeron Plus kit was not inferior to BOVIGAM  in performance. High lymphocyte numbers in blood did not impact on TB-Feron Plus results, while delayed time before culture interfered with OD value.

KCI등재

2경남지역 사육 염소 큐열 항체 양성률 조사

저자 : 성민호 ( Min-ho Seong ) , 박종식 ( Jong-sik Bak ) , 윤도경 ( Do-kyung Youn ) , 김형수 ( Hyeong-su Kim ) , 고병효 ( Byeong-hyo Ko ) , 함정민 ( Jeong-min Ham ) , 정명호 ( Myeong-ho Jeong )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 211-216 (6 pages)

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Coxiella burnetii is the causative agent of Q fever which is a zoonosis occuring in both humans and animals worldwide. The purpose of this study was to investigate the seroprevalence of C. burnetii in Korean native goat in Gyeongnam province, Korea. A total of 1,365 goat blood samples from 273 farms in Gyeongnam province were collected between 2018 and 2019. Among them, 177 (13.0%) samples out of 71 (26.0%) farms were seropositive for C. burnetii by ELISA. Seroprevalence were 15.4% and 10.9% in 2018 and 2019, respectively. According to the region, seroprevalence in western, central, eastern, northern and southern areas of Gyeongnam province were 16.6%, 17.8%, 8.0%, 11.6% and 10.8%, respectively. Seroprevalence was increased with breeding scale (Head<10:7.0%, 10≤Head<50:8.7%, 50≤Head<100:13.6%, 100≤Head:28.8%). Seroprevalence according to the season showed highest in summer (18.9%) and lowest in winter (9.4%). These results indicated that C. burnetii infection is widespread among Korean native goats of Gyeongnam province in Korea and further study needs to prevent the circulation of other livestock with Korean native goat.

KCI등재

3경기지역 유기견의 주요 전염성 질병 실태 조사

저자 : 고재형 ( Jae-hyung Ko ) , 박미영 ( Mi-young Park ) , 신병훈 ( Byung-hoon Shin ) , 남영희 ( Young-hee Nam ) , 구경녀 ( Kyung-nyer Ku ) , 손주일 ( Ju-ill Son )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 217-225 (9 pages)

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This study was conducted to survey pathogens of canine coronavirus (CCV), canine distemper virus (CDV), canine influenza virus (CIV), canine parvovirus (CPV), severe fever with thrombocytopenia syndrome virus (SFTSV), Dirofilaria (D.) immitis, Giardia and antibodies against Anaplasma (A.) phagocytophilum, Borrelia (B.) burgdorferi, Brucella (B.) canis and Ehrlichia (E.) canis among stray dogs in Gyeonggi province. We collected 271 feces, 291 bloods, 311 nasal and ocular swab samples from 311 of dogs in the Gyeonggi province assistance dogs sharing center from January to December, 2019. Among canine infectious disease pathogens, Giardia was highly detected in 46/271 (17.0%) samples. Subsequently, CCV 10.3% (28/271), D. immitis 8.2% (24/291), CPV 4.1% (11/271), CDV 1.0% (3/311), A. phagocytophilum (antibody) 0.3% (1/291), E. canis (antibody) 0.3% (1/291) were detected. Based on the results, this study is expected to provide a useful reference for disease control and management of stray dogs.

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43D8 scFv 형질전환 돼지 개발 및 PRRS 저항성 평가

저자 : 이휘철 ( Hwi-cheul Lee ) , 이건섭 ( Gunsup Lee ) , 김지윤 ( Ji-yoon Kim ) , 양현 ( Hyeon Yang ) , 이보람 ( Bo Ram Lee ) , 박미령 ( Mi-ryung Park ) , 황인설 ( In-sul Hwang ) , 이풍연 ( Poongyeon Lee ) , 변승준 ( Sung-june Byun ) , 김원일 ( Won-il Kim ) , 오건봉 ( Keon Bong Oh )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 227-236 (10 pages)

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In this study, we have developed 3D8 scFv transgenic pig (TG) by microinjection of fertilized one-cell pig zygotes (2.17%). The effect of 3D8 scFv TG on porcine reproductive and respiratory syndrome virus (PRRSV) resistance were evaluated through PRRSV VR2332 (1×10 3 TCID50/mL) challenge and transmission experiments. As a result, the average daily weight gain (ADWG) of TG increased compared to the wild type pigs (WT) in PRRSV challenge groups and the serum viremia levels of the TG was significantly lower than of WT on the 7 day and 21 day after infection, meaning that the viral shedding was suppressed by 3D8 scFv expression. These results suggest that the expression of 3D8 scFv in pig could suppress spreading of infected virus to pigs sharing a room.

KCI등재

5Development of sandwich enzyme-linked immunosorbent assay for a large-scale detection of porcine transmissible gastroenteritis virus in feces

저자 : Yeonsu Oh , Sang-joon Lee , Ho-seong Cho , Dongseob Tark

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 237-244 (8 pages)

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Porcine transmissible gastroenteritis (TGE) has been a significant cause of economic losses in pig farming industry since 1950s. Although transmissible gastroenteritis virus (TGEV) has declined in recent years, it should not be excluded because of its characteristics; the frequency of gene mutation, the mortality in piglets, and the possibility for sudden incidence. Therefore, the herd-level monitoring of the virus is important to prevent further circulation of TGE. The aim of this study is to develop a large-scale sandwich enzyme-linked immunosorbent assay (ELISA) with high specificity to rapidly detect TGEV in feces by using monoclonal antibodies (Mabs). The TGEV specific Mabs were produced in hybridoma cells. Among the Mabs belonged to the IgG class developed by this study, the final selected 8H6, 1B7, 4G3, and 1F8 were identified to have the neutralization ability against TGEV. The sandwich ELISA was established using 8H6 as a reporter antibody and 1B7 and the reported 5C8 as a capture antibody. The developed sandwich ELISA was able to distinguish TGEV from other pathogenic diarrheal agents (porcine rotavirus, porcine reovirus, porcine epidemic diarrhea virus (PEDV), E. coli, and C. perfringens) in tissue culture as well as fecal samples. And the detection rate of TGEV in feces was 80% compared with RT-PCR. The results suggested that the developed sandwich ELISA may be useful in the herd-level monitoring for effective preventive measures due to the early diagnosis of TGEV using a large amount of samples.

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6Hematological and microbial analysis on a Holstein heifer with infectious bovine keratoconjunctivitis

저자 : Seungmin Ha , Taiyoung Hur , Seogjin Kang , Younghun Jung , Junkyu Son , Donghyeon Kim , Jihwan Lee , Hyunhoon Sung , Eunseok Cho , Sangbeom Kim

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 245-250 (6 pages)

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Infectious bovine keratoconjunctivitis (IBK) is the most common ocular disease in cattle, manifesting as corneal opacity, corneal ulcerations and potentially vision loss. The present report describes a 10- month-old Holstein Friesian heifer with IBK treated by systemic tulathromycin, and subconjunctival injection of penicillin and dexamethasone. We investigated changes in the hematological indices and microorganisms related to IBK after treatment. Neutrophils and monocytes decreased during recovery, so it was assumed that these two types of white cells are associated with IBK. Moraxella bovoculi was cleared in the eye, nasal cavity, and oral cavity after treatment. The distribution of M. bovoculi before treatment indicated that a combined systemic and subconjunctival treatment was necessary. The lesioned eye was found to be overwhelmed by Mycoplasma bovoculi, while pathogen abundance was reduced in the nasal cavity and oral cavities. These results suggest that antibiotic treatment can alter the composition and relative abundance of microorganisms.

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7Co-infection of Toxoplasma gondii and porcine reproductive and respiratory syndrome virus in suckling piglets in Jeju, Korea

저자 : Young-min Choi , Hyoung-seok Yang , Jae-hoon Kim

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 251-256 (6 pages)

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Two suckling piglets, 4 days and 10 days of age, showed lethargy and dyspnea after birth and mortality had been increased after incoming gilts from breeding farm. At necropsy, the lungs showed diffuse fail to collapse with rubbery consistency, edematous dilatation of interlobular septa, and lobular consolidation with purple red color. Heart was diffuse pale in color and had several irregular linear-shaped macules or patches. Histopathologically, diffuse interstitial pneumonia with the proliferation of type II pneumocytes was present in the lungs of 2 piglets. Alveolar lumens contained necrotic cellular debris derived from neutrophils and macrophages. Multifocal hemorrhage and necrotizing pneumonia with protozoan tachyzoites were observed in the lungs. Severe multifocal to confluent necrotic myocarditis, necrotic encephalitis, and necrotic adrenalitis with intralesional protozoan tachyzoites were observed in piglets. According to immunohistochemical analysis (IHC), Toxoplasma (T.) gondii tachyzoites antigens were confirmed in lung, heart, brain, and adrenal gland. And porcine reproductive and respiratory syndrome virus (PRRSV) antigens were also detected in the cytoplasm of macrophages in lungs using IHC. Based on the gross, histopathologic and immunohistochemical features, two suckling piglets were diagnosed as co-infection of T. gondii and PRRSV.

KCI등재

8경기도 산란계에서 Ornithobacterium rhinotracheale의 높은 항체 양성률

저자 : 정광 ( Kwang Jung )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 257-259 (3 pages)

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Ornithobacterium rhinotracheale (ORT) causes pneumonia, airsacculitis, and pleuritis in chickens and other avian species. Little is known about the seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea. The purpose of this study was to determine the seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea from May to September 2019. A total of 460 chickens in 28 flocks were tested for antibodies to ORT by using commercial enzyme-linked immunosorbent assay (ELISA) kit. The seroprevalence of ORT antibodies in the flocks was 100% (28/28) and the overall seroprevalence in individual chickens was 98.91% (455/460). This survey indicated the high seroprevalence of ORT in layer chickens in Gyeonggi province, South Korea. Therefore, measures should be executed to control ORT in layer chickens in this province.

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9Determination of cyromazine in commercial insecticides using HPLC-DAD

저자 : Young-wook Kim , Bok Hee Han , Young Eun Kang , Chae Hong Rhee , Sang-ji Seo , Soohee Kim , Wooseog Jeong , Moon Her

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 261-265 (5 pages)

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Each commercial cyromazine insecticide has different HPLC conditions. The aim of this study was to establish a standardized chromatographic method for the determination of cyromazine in commercial insecticides. The separation was achieved on two C18 columns - Waters  Bondapak C (4×300 nm i.d., 10 μm) and X bridge (4.6×250 nm i.d., 5 μm) using a mobile phase composed of water/methanol/ ethanolamine (76:24:0.1, v/v), with UV detection at wavelengths 230 nm and 254 nm. A total of six commercial cyromazine insecticides were analyzed. In this study, the optimal high-performance liquid chromatography conditions for the analysis of cyromazine were as follows: a mobile phase of water/ methanol/ethanolamine (76:24:0.1, v/v) at a flow rate of 1.0 mL/min and a detection wavelength of 230 nm using a X bridge C18 column (4.6×250 nm i.d., 5 μm) at a column temperature of 25°C. The calibration curve was linear in the concentration range of 5∼50 μg/mL, with a correlation coefficient of 0.99995. The cyromazine detection limit was 0.2 μg/mL, and the limit of quantification was 0.59 μg/mL. The percentage recovery ranged from 99.8% to 101.0% for cyromazine, and the relative standard deviation was not over 2.0%. The cyromazine concentration ranged from 92.7% to 109.4% and was within the acceptable range (90∼120%) for the percent of the labeled amount. This method was found to be suitable for determining cyromazine in commercial insecticides.

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10가축 전염병 관리를 위한 농장 질병관리등급제 개선 방안

저자 : 조호성 ( Ho-seong Cho )

발행기관 : 한국동물위생학회(구 한국가축위생학회) 간행물 : 한국가축위생학회지 (KOJVS) 43권 4호 발행 연도 : 2020 페이지 : pp. 267-271 (5 pages)

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The emergence of highly contagious viral diseases of livestock and poultry such as foot-and-mouth disease (FMD), African swine fever (ASF) and highly pathogenic influenza (HPAI) have necessitated the formulation of policies and regulatory frameworks for preventing ingress of exotic diseases and controlling dissemination of endemic diseases within the country. The disease control ratings to manage contagious livestock disease in farms have not being widely used because the evaluation parameters were not being considered inappropriate. The purpose of this study was to reinforce the validity of the parameters for evaluation factors and the objectivity of the scoring criteria in the application of the current disease management rating system. The diseases subject to intensive management were improved by reflecting farm situation. For the evaluation based on the disease grading system of farms, the diseases subject to intensive management were realized, the list of diseases for evaluating the vaccination rate was revised, and the quarantine and sanitation management standards were revised. The effectiveness of the improved livestock disease management system was confirmed through the application of actual pig farms. Therefore, this system will be used as a means to evaluation the biosecurity at the farm units. Through this, it will contribute to establish effective biosecurity measures for each farm by improving and supplementing the present disease grading system.

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1연안해역에서 석유오염물질의 세균학적 분해에 관한 연구

(2006)홍길동 외 1명심리학41회 피인용

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(2006)홍길동심리학41회 피인용

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(2006)홍길동41회 피인용

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