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한국미생물·생명공학회지 update

Microbiology and Biotechnology Letters

  • : 한국미생물생명공학회(구 한국산업미생물학회)
  • : 자연과학분야  >  생물
  • : KCI등재
  • : SCOPUS
  • : 연속간행물
  • : 계간
  • : 1598-642x
  • : 2234-7305
  • : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)

수록정보
수록범위 : 30권1호(2002)~46권3호(2018) |수록논문 수 : 973
한국미생물·생명공학회지
46권3호(2018년 09월) 수록논문
최근 권호 논문
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KCI등재 SCOPUS

1생물학적 하폐수처리과정에서 N2O 배출 및 저감에 관한 고찰

저자 : 조경숙 ( Kyung-suk Cho )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 181-193 (13 pages)

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Nitrous oxide (N2O) is a potent greenhouse gas as well as an ozone-depleting substance. N2O is emitted during the biological nitrogen removal process in wastewater treatment systems (WTSs), and has significant environmental impacts. In this study, N2O emission in WTSs was comprehensively reviewed to better understand the effects of key parameters on N2O emission and obtain useful guidelines for N2O mitigation strategies in WTSs. Three biological pathways leading to N2O emission are hydroxylamine oxidation, nitrifier denitrification, and heterotrohic denitrification. Measurements at lab-, pilot- and full-scale WTSs have shown large variations in N2O emission (0-95% of N-loaded) during wastewater treatment. In the full-scale WTSs (0-14.6% N2O of N-loaded), the average and median values were 1.95% and 0.2% of N-loaded, respectively. Dissolved oxygen, nitrite concentrations, and chemical oxygen demand (COD)/N ratio are the most important parameters leading to N2O emission. A variety of operational strategies have been suggested to minimize N2O emission from WTSs. A new N2O mitigation strategy involving the introduction of microorganisms with high N2O reductase activity or oxygenic denitrification ability has been proposed as an alternative canonical denitrification.

KCI등재 SCOPUS

2Optimized M9 Minimal Salts Medium for Enhanced Growth Rate and Glycogen Accumulation of Escherichia coli DH5α

저자 : Liang Wang , Qinghua Liu , Yangguang Du , Daoquan Tang , Micha

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 194-200 (7 pages)

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Glycogen plays important roles in bacteria. Its structure and storage capability have received more attention recently because of the potential correlations with environmental durability and pathogenicity. However, the low level of intracellular glycogen makes extraction and structure characterization difficult, inhibiting functional studies. Bacteria grown in regular media such as lysogeny broth and tryptic soy broth do no accumulate large amounts of glycogen. Comparative analyses of bacterial media reported in literature for glycogen-related studies revealed that there was no consistency in the recipes reported. Escherichia coli DH5α is a convenient model organism for gene manipulation studies with respect to glycogen. Additionally, M9 minimal salts medium is widely used to improve glycogen accumulation, although its composition varies. In this study, we optimized the M9 medium by adjusting the concentrations of itrogen source, tryptone, carbon source, and glucose, in order to achieve a balance between the growth rate and glycogen accumulation. Our result showed that 1 × M9 minimal salts medium containing 0.4% tryptone and 0.8% glucose was a well-balanced nutrient source for enhancing the growth and glycogen storage in bacteria. This result will help future investigations related to bacterial physiology in terms of glycogen function.

KCI등재 SCOPUS

3Isolation of Streptococcus thermophilus and Lactobacillus delbrueckii as Starter Culture Candidate Originated from Indonesian Cow's Milk

저자 : Danish Andrian , Denny Rizkinata , Tan Steven Ryan Susanto , Jap L

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 201-209 (9 pages)

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Streptococcus thermophilus, Lactobacillus delbrueckii, Lactobacillus fermentum and Lactobacillus casei were successfully isolated from indigenous Indonesian fresh milk based on the general morphological and biochemical classification as described in Bergey's manual. Verification was conducted by sequencing of 16S rRNA after selection using the classification method mentioned in the manual. All isolates exhibited antimicrobial activity against Escherichia coli and Staphylococcus aureus in the well diffusion test. The susceptibilities of the isolated S. thermophilus 24/S1 and L. delbrueckii 94/L4 against 22 different antibiotics were determined by the disc diffusion method and variable susceptibility patterns were observed. Both isolates were susceptible to amoxicillin, the most commonly prescribed antibiotic, and resistant to sulfonamide. The presence of a plasmid was not detected after extraction. S. thermophilus 24/S1 and L. delbrueckii 94/L4 starter cultures were prepared for yogurt production after 9.5 h of incubation and the yogurt was evaluated for its flavor and quality by 30 volunteers. A score of 4.93 ± 0.45 out of 7 was obtained as compared to the yogurt prepared using commercial starter cultures which yielded a score of 4.76 ± 0.30 out of 7.

KCI등재 SCOPUS

4Microbiota and Physicochemical Analysis on Traditional Kocho Fermentation Enhancer to Reduce Losses (Gammaa) in the Highlands of Ethiopia

저자 : Adane Hailu Dibaba , Ashenafi Chaka Tuffa , Endrias Zewdu Gebremedhin

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 210-224 (15 pages)

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Warqe (Ensete ventricosum) has been traditionally fermented in an earthen pit to yield a carbohydrate-rich food product named kocho, for generations. A fermentation enhancer (gammaa) was added to this fermenting mass to enhance the fermentation process. The objectives of this study were to assess the physicochemical properties and microbiota of the kocho fermentation enhancer culture to reduce losses. Cross-sectional study design was implemented to collect 131 gammaa samples on the first day of fermentation. The samples were further classified into four groups according to the duration of fermentation (14, 21, 30, and 60 days) practised in various households traditionally. The results showed that the fermentation time significantly affected the physicochemical properties and microbial load of gammaa (p < 0.01). As the fermentation progressed from day 1 to 60, the pH decreased and the titratable acidity increased. The total coliform, Enterobacteriaceae, aerobicmesophilic bacteria (AMB), yeast, and mould counts were significantly reduced at the end of fermentation. In contrast, the number of lactic acid bacteria (LAB) increased significantly until day 30 of fermentation, because of the ability of the LAB to grow at low pH. Lactobacillus species from LAB isolates and Enter obacteriaceae from AMB isolates were the most abundant microorganisms in gammaa fermentation. However, the Enterobacteriaceae and Lactobacilli species count showed decreasing and increasing trends, respectively, as the fermentation progressed. These isolates must be investigated further to identify the species and strain, so as to develop gammaa at the commercial scale.

KCI등재 SCOPUS

5Estimation of Antibacterial Properties of Chlorophyta, Rhodophyta and Haptophyta Microalgae Spe

저자 : Khawaja Muhammad Imran Bashir , Jae-hyeon Lee , Maike Julia Petermann

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 225-233 (9 pages)

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In this exploratory study, eight types of microalgae from different phyla (Chlamydomonas reinhardtii, Chlorella species, Haematococcus pluvialis, Porphyridium purpureum, Porphyridium cruentum, Isochrysis species, Isochrysis galbana, and Pavlova lutheri) were tested for their antibacterial activities against eight target pathogenic bacterial strains. The agar well diffusion method and broth micro dilution assay were conducted to estimate the antibacterial activity. Microalgae cell-free supernatants, exopolysaccharides (EPS), water, and organic solvent extracts were used for inhibition analysis. EPS extracted from P. lutheri showed activity against Bacillus subtilis and Pseudomonas aeruginosa. Inhibition zone diameters of 14- 20 mm were recorded on agar plates, while the minimum inhibitory concentrations in the broth micro dilution assay were 0.39-25 mg ml-1. During this study, haptophyte microalgae, Isochrysis species, and P. lutheri extracts showed the highest activity against most of the tested pathogenic bacterial strains, while most of the extracts were active against the important foodborne pathogen P. aeruginosa. This study showed promising results regarding important microalgae phyla, which will further aid research related to extracts and exploitation of bioactive metabolic compounds in the food and pharmaceutical industries.

KCI등재 SCOPUS

6남극 로스해에서 분리한 Croceibacter atlanticus 균 유래 리파아제의 생산, 고정화, 효소특성 연구

저자 : 박채경 ( Chae Gyeong Park ) , 김형권 ( Hyung Kwoun Kim )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 234-243 (10 pages)

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The Antarctic Ocean contains numerous microorganisms that produce novel biocatalysts that can have applications in various industries. We screened various psychrophilic bacterial strains isolated from the Ross Sea and found that a Croceibacter atlanticus strain (Stock No. 40-F12) showed high lipolytic activity on a tributyrin plate. We isolated the corresponding lipase gene (lipCA) by shotgun cloning and expressed the LipCA enzyme in Escherichia coli cells. Homology modeling of LipCA was carried out using the Spain Arreo lake metagenome alpha/beta hydrolase as a template. According to the model, LipCA has an α/β hydrolase fold, Gly-X-Ser-X-Glymotif, and lid sequence, indicating that LipCA is a typical lipase enzyme. Active LipCA enzyme was purified fromthe cell-free extract by ammonium sulfate precipitation and gel filtration chromatography. We determined its enzymatic properties including optimum temperature and pH, stability, substrate specificity, and organic solvent stability. LipCA was immobilized by the cross-linked enzyme aggregate (CLEA) method and its enzymatic properties were compared to those of free LipCA. After cross-linking, temperature, pH, and organic solvent stability increased considerably, whereas substrate specificities did not changed. The LipCA CLEA was recovered by centrifugation and showed approximately 40% activity after 4th recovery. This is the first report of the expression, characterization, and immobilization of a C. atlanticus lipase, and this lipase could have potential industrial application.

KCI등재 SCOPUS

7Recombinant Protein Disulfide Isomerase A3 with an Elongated Peptide Tag Production Process Using Escherichia coli

저자 : Kwang-jin Kim , Sung-hwan You , Yongjin Lee , Chan Mi Park , G

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 244-252 (9 pages)

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Protein disulfide isomerase A3 (PDIA3) is a major member of the protein disulfide isomerase (PDI) family. PDI proteins commonly reside in the endoplasmic reticulum and mediate important thiol-disulfide interchanges during post-translational protein folding. Unlike other PDI family members, PDIA3 is ubiquitous in various organ systems. However, its physiological activity varies in other tissues. PDIA3 has been associated with cancer, airway inflammation, neurodegenerative diseases, and metabolic diseases. However, the mechanisms of the association of PDIA3 with these pathological conditions remain unclear. Recombinant PDIA3 (rPDIA3) is needed to clarify the interactions between PDIA3 and certain physiological phenomena. In the present study, we aimed to produce highly purified rPDIA3 for use in pathological experiments. We expressed rPDIA3 with a histidine-enriched elongated peptide tag in Escherichia coli and obtained rPDIA3 at 97.8% purity using consecutive His-tag and reverse-phase chromatography. Elongated peptide tags screened from artificially designated library had dual functions for protein expression and simple purification.

KCI등재 SCOPUS

8유용한 바실러스의 토양 접종에 따른 토착 세균 군집의 변화

저자 : 김이슬 ( Yiseul Kim ) , 김상윤 ( Sang Yoon Kim ) , 안주희 ( Ju Hee An ) , 상미경 ( Mee Kyung

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 253-260 (8 pages)

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Beneficial microorganisms are widely used in the forestry, livestock, and, in particular, agricultural sectors to control soilborne diseases and promote plant growth. However, the industrial utilization of these microorganisms is very limited, mainly due to uncertainty concerning their ability to colonize and persist in soil. In this study, the survival of beneficial microorganisms in field soil microcosms was investigated for 13 days using quantitative PCR with B. subtilis group-specific primers. Bacterial community dynamics of the treated soils were analyzed using 16S ribosomal RNA (rRNA) gene amplicon sequencing on the Illumina MiSeq platform. The average 16S rRNA gene copy number per g dry soil of Bacillus spp. was 4.37 × 106 after treatment, which was 1,000 times higher than that of the control. The gene copy number was generally maintained for a week and was reduced thereafter, but remained 100 times higher than that of the control. Bacterial community analysis indicated that Acidobacteria (26.3 ± 0.9%), Proteobacteria (24.2 ± 0.5%), Chloroflexi (11.1 ± 0.4%), and Actinobacteria (9.7 ± 2.5%) were abundant phyla in both treated and non-treated soils. In the treated soils, the relative abundance of Actinobacteria was lower, whereas those of Bacteroidetes and Firmicutes were higher compared to the control. Differences in total relative abundances of operational taxonomic units belonging to several genera were observed between the treated and non-treated soils, suggesting that inoculation of soil with the Bacillus strains influenced the relative abundances of certain groups of bacteria and, therefore, the dynamics of resident bacterial communities. These changes in resident soil bacterial communities in response to inoculation of soil with beneficial Bacillus spp. provide important information for the use of beneficial microorganisms in soil for sustainable agriculture.

KCI등재 SCOPUS

9Distinct Genetic Variation of Helicobacter pylori cagA, vacA, oipA, and sabA Genes in Thai and Korean Dyspeptic Patients

저자 : Wongwarut Boonyanugomol , Worrarat Kongkasame , Prasit Palittapongarnp

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 261-268 (8 pages)

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Differences in host ethnicities and geographical distributions may influence the genetic variation and pathogenesis of Helicobacter pylori strains, particularly with respect to those with a high risk of gastric cancer and in Asian Enigma regions. We simultaneously identified H. pylori virulence-associated genes involved in inflammation and cell damage in Thai and Korean dyspeptic patients. The virulence-associated gene cagA, cagA genotypes (East Asian and Western type cagA), vacA genotypes (s- and m-), oipA, and sabA were detected in Thai and Korean dyspeptic patients by polymerase chain reaction (PCR), real-time PCR, and DNA sequence analysis. Comparisons between the two regions showed that cagA, East Asian type cagA, and vacA s1/m1 in Korean dyspeptic patients occurred at rates of 100%, 86.67%, and 88.89%, respectively (p < 0.05). The oipA- and sabA-positive samples were significantly more predominant in the Korean population (95.56%, 91.11%) than in the Thai population (32%, 34%). DNA sequence analysis revealed differences in the patterns of cytosine-thymine dinucleotide repeats of oipA and sabA among the two populations of dyspeptic patients. Our results indicate that the H. pylori strains detected in the two regions were divergent, and strains colonizing the Korean dyspeptic patients may be more virulent than those in the Thai population. Our data may help explain H. pylori pathogenesis in Asian Enigma areas with a low gastric cancer incidence. However, other factors involving H. pylori infection in these two regions should be further analyzed.

KCI등재 SCOPUS

10Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

저자 : Dong-kun Yang , Ha-hyun Kim , Jieun Ryu , Mi-ryun Gee , In-soo

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 46권 3호 발행 연도 : 2018 페이지 : pp. 269-276 (8 pages)

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Rabies virus (RABV)-specific antibodies in animals and humans are measured using standard methods such as fluorescent antibody virus neutralization (FAVN) tests and rapid fluorescent focus inhibition tests, which are based on cell culture systems. An alternative assay that is safe and easy to perform is required for rapid sero-surveillance following mass vaccination of animals. Two purified monoclonal antibodies (4G36 and B2H17) against RABV were selected as capture and detection antibodies, respectively. A genetically modified RABV, the ERAGS strain, was propagated and concentrated by polyethylene glycol precipitation. Optimal conditions for the RABV antigen, antibodies, and serum dilution for a blocking enzymelinked immune sorbent assay (B-ELISA) were established. We evaluated the sensitivity, specificity, and accuracy of the B-ELISA using serum samples from 138 dogs, 71 raccoon dogs, and 25 cats. The B-ELISA showed a diagnostic sensitivity of 95.8-96.3%, specificity of 91.3-100%, and accuracy of 96.0-97.2% compared to the FAVN test. These results suggest that the B-ELISA is useful for sero-surveillance of RABV in dogs, raccoon dogs, and cats.

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1연안해역에서 석유오염물질의 세균학적 분해에 관한 연구

(2006)홍길동 외 1명심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동41회 피인용

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