간행물

한국미생물·생명공학회지 update

Microbiology and Biotechnology Letters

  • : 한국미생물생명공학회(구 한국산업미생물학회)
  • : 자연과학분야  >  생물
  • : KCI등재
  • : SCOPUS
  • : 연속간행물
  • : 계간
  • : 1598-642x
  • : 2234-7305
  • : 산업미생물학회지(~2001)→한국미생물·생명공학회지(2002~)

수록정보
수록범위 : 30권1호(2002)~47권3호(2019) |수록논문 수 : 1,044
한국미생물·생명공학회지
47권3호(2019년 09월) 수록논문
최근 권호 논문
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KCI등재 SCOPUS

1Lactobacillus rhamnosus BHN-LAB 76로 발효한 흰목이버섯(Tremella fuciformis Berk) 추출물의 항비만 및 항당뇨 효과

저자 : 윤여초 ( Yeo-cho Yoon ) , 김병혁 ( Byung-hyuk Kim ) , 김중규 ( Jung-gyu Kim ) , 이준형 ( Jun-hyeong Lee ) , 박예은 ( Yeeun Park ) , 박혜숙 ( Hye-suk Park ) , 황학수 ( Hak-soo Hwang ) , 권기석 ( Gi-seok Kwon ) , 이중복 ( Jung-bok Lee )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 323-331 (9 pages)

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흰목이버섯(Tremella fuciformis Berk.; TF)은 흰목이과에 속하는 버섯으로 한국, 중국 및 열대지방에 분포한다. 흰목이버섯은 아시아 전통 의학에서 고혈압, 노화, 암 및 동맥 경화를 예방하는 것으로 알려져 있다. 본 연구는 L. rhamnosus BHN-LAB 76로 발효된 흰목이버섯 추출물의 항 당뇨 효능에 대해 조사하였다. 그 결과, 발효된 흰목이버섯 추출물은 발효하지 않은 추출물에 비해 α-glucosidase 저해 활성을 증가시키고, 3T3-L1 전지방 세포에서의 지방세포 분화 유도를 통한 지방구 생성을 억제하는 것을 확인하였다. 이러한 발효된 흰목이버섯은 지방 및 세포 분화 유도에 관여한다고 알려진 AMPK, Akt의 유전자 발현을 촉진하고, JNK의 발현을 억제하는 것을 통해 지방생성억제 및 항 당뇨 활성이 증가됨을 확인하였다. 따라서 L. rhamnosus BHN-LAB 76으로 발효한 흰목이버섯 추출물은 항비만 및 항당뇨 기능성 소재 및 식품 개발로의 활용이 가능할 수 있음을 제안한다.


White jelly fungus (Tremella fuciformis Berk; TF) has been used as a traditional medicine in Asia; it is known to prevent hypertension, aging, cancer, and arteriosclerosis. This study aimed to investigate the anti-diabetic effects of fermented Tremella fuciformis Berk (FTF) ethanol extracts fermented with L. rhamnosus BHN-LAB 76. We show that FTF increases the α-glucosidase inhibitory activity and suppress the adipogenesis of 3T3-L1 adipocytes. These inhibitory effects of FTF are accompanied by the regulation of the phosphorylation of AMPK, JNK, and Akt. These data demonstrate that FTF not only inhibits adipogenesis by affecting the adipogenic signaling, but also increases the anti-diabetic effects by regulating the insulin signaling pathway. Therefore, we suggest that the FTF can be used for developing functional food and cosmetics materials.

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2포장재 조건에 따른 365 nm UV-LED 조사의 Bacillus subtilis 생육 억제 효과

저자 : 이다혜 ( Da-hye Lee ) , 정소미 ( So-mi Jeong ) , 쉬시아오통 ( Xiaotong Xu ) , 김꽃봉우리 ( Koth-bong-woo-ri Kim ) , 안동현 ( Dong-hyun Ahn )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 332-336 (5 pages)

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본 연구에서는 그람 양성의 호기성 유포자 세균 중 자연계에 널리 분포되어 식품에 문제를 일으키는 대표적인 균인 B. subtilis를 대상으로 365 nm UV-LED의 생육 억제 효과를 입증하였다. 또한 365 nm UV-LED 조사 시, 식품 포장재로 흔히 사용되고 있는 유리, LLD-PE, Nylon/LDPE 및 PS 등의 포장 조건에 따른 B. subtilis 생육 억제 효과를 확인하였다. 그 결과, B. subtilis의 생육 억제 효과가 가장 뛰어난 재질은 Nylon/LDPE와 LLD-PE로 확인되었고, 대조구의 생존율인 -log 5 값과 비교하여 각각의 생존율은 약 -log 2.5-2.9, -log 2.58-3.61로 나타났다. 이 때 재질의 두께가 미생물의 생육에 미치는 영향은 미미한 것으로 관찰되었고, 포장 재질에 따라 365 nm UV-LED 투과력이 다르다는 것을 확인할 수 있었다. 통상적으로 log 3 이상 생균수가 감소하면 99.9% 살균효과가 있는 것으로 나타낼 수 있는데, 본 연구를 통해 365 nm UV-LED가 흔히 사용되고 있는 식품 포장재를 투과하여 균의 생육 억제에 영향을 줄 수 있음을 확인 하였다. 이러한 결과를 통해 365 nm UV-LED의 사용이 식품보존과 식품산업 분야의 응용기술로써 잠재력이 있음을 시사하는 바이다.


The use of ultraviolet (UV) spectroscopy for foods is known to have a microbial inhibitory effect. UV-A having a longer wavelength than UV-C can be used for continuous or intermittent UV irradiation of food stored in containers or packages. Because UV-LED can be used effectively at a low price, this study reported the effect of UV-A 365 nm-LED on inhibiting Bacillus subtilis in accordance with the packaging conditions employed in daily use. The packaging materials were linear low-density polyethylene (LLD-PE), nylon/low density polyethylene (LDPE), polystyrene, and glass. When all packaging materials were treated with 365 nm UV-LED, B. subtilis was observed to remain inactive for 30-60 min. Further, compared with the control (-log 5), the survival rate of B. subtilis was -log 2.0-2.5 for nylon/LDPE and -log 2.58-3.61 for LLDPE. These packaging materials showed an excellent inhibitory effect regardless of their thickness. Typically, a decrease in the viable cell count of more than 3 log indicates a 99.9% bactericidal effect. These results suggest that 365 nm UV-LED permeated the packaging material and inhibited bacterial growth.

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3재조합 Corynebacterium glutamicum으로부터 헴첼 생산에 미치는 프로모터의 효과

저자 : 양형모 ( Hyungmo Yang ) , 김필 ( Pil Kim )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 337-342 (6 pages)

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We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase (hemA+) under control of a constitutive promoter (P180) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the hemA+ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The hemA+ expression by PgapA (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by PH36 (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control (hemA+ expression by P180 constitutive promoter). The hemA+ expression by a late log-phase activating Psod (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The hemA+ expression led by a heat-shock responding chaperone promoter (PdnaK) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter strength and induction phase are discussed based on the results for the heme production at an industrial scale.

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4Some Properties and Microbial Community Changes of Gul (Oyster) Jeotgal during Fermentation

저자 : Jeong A Kim , Zhuang Yao , Hyun-jin Kim , Jeong Hwan Kim

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 343-349 (7 pages)

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Gul jeotgals (GJs) were prepared using solar salt aged for 3 years. One sample was fermented using starters, such as Bacillus subtilis JS2 and Tetragenococcus halophilus BS2-36 (each 106 CFU/g), and another sample was fermented without starters for 49 days at 10℃. Initial counts of bacilli and lactic acid bacteria (LAB) in non-starter GJ were found to be 3.20 × 102 and 7.67 × 101 CFU/g on day 0, and increased to 1.37 × 103 and 1.64 × 106 CFU/g on day 49. Those of starter GJ were found to be 2.10 × 105 and 3.30 × 107 CFU/g on day 49, indicating the growth of starters. The pH values of GJ were 5.93 ± 0.01 (non-starter) and 5.92 ± 0.01 (starter) on day 0 and decreased to 5.78 ± 0.01 (non-starter) and 5.75 ± 0.01 (starter) on day 49. Amino-type nitrogen (ANN) production increased continuously during fermentation, and 407.19 ± 15.85 (non-starter) and 398.04 ± 13.73 (starter) mg% on day 49. Clone libraries of 16S rRNA genes were constructed from total DNA extracted from non-starter GJ on days 7, 21, and 42. Nucleotide sequences of Escherichia coli transformants harboring recombinant pGEM-T easy plasmid containing 16S rRNA gene inserts from different bacterial species were analyzed using BLAST. Uncultured bacterium was the most dominant group and Gram - bacteria such as Acidovorax sp., Afipia sp., and Variovorax sp. were the second dominant group. Bacillus amyloliquefaciens (day 7), Bacillus velezensis (day 21 and 42), and Bacillus subtilis (day 42) were observed, but no lactic acid bacteria were detected. Acidovorax and Variovorax species might play some role in GJ fermentation. Further studies on these bacteria are necessary.

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The transcription factor, GATA-1, plays an important role in the FcεRI α chain expression in mast cells and basophils. This study was conducted to investigate the downregulation of the transcription factor GATA-1 by kaempferol isolated from Nelumbo nucifera stamens in FcεRI-mediated allergic reactions. Kaempferol inhibited FcεRI-mediated histamine release. Western blotting analysis and RT-PCR showed that the protein and mRNA expression of GATA-1 was suppressed by kaempferol in a dose-dependent manner. These results suggest that kaempferol may inactivate basophils by downregulating the FcεRI α chain expression via the inhibition of the GATA-1 expression.

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6Pyrosequencing을 이용한 메주, 천일염, 된장의 곰팡이 군집 분석

저자 : 이림기 ( Limgi Lee ) , 허소정 ( Sojeong Heo ) , 정도원 ( Do-won Jeong )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 354-358 (5 pages)

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메주를 염수에 침지하여 된장을 만드는 과정 동안 진균의 군집 변화와 재료가 된 메주와 천일염의 균총 천이를 확인하고자 진균의 ITS (internal transcribed spacer) 유전자 서열을 기반으로 하는 pyrosequencing을 통해 미생물 군집을 확인하였다. 된장의 주요 원료인 메주는 Botrytis spp. (57.94%)와 Dothiorella samentorum (24.08%)가 우점종으로 확인되었다. 천일염은 확인되지 않은 종들이(70.13%) 검출되었고, 그 외 종들은 적은 비율로 다양하게 검출되었다. 된장은 진균 중 곰팡이가 아닌 효모인 Candida versatilis (92.62%)가 가장 높은 우점진균으로 확인되었다. 메주의 저염은 곰팡이 생육이 용이 했고, 천일염은 고세균 또는 호염진균, 된장은 고염 효모의 생존에 용이했던 것으로 판단된다. 메주와 천일염의 우점진균이 된장의 균총에 직접적인 영향을 미치지 못하는 것으로 판단된다.


In order to evaluate the migration of fungi into doenjang from its materials, meju and solar salt, microbial communities were analyzed using pyrosequencing. Dominant fungi of meju were Botrytis spp. (57.94%) and Dothiorella samentorum (24.08%). Unidentified fungal species (37.53%), unassigned species (32.60%) and several fungal species of small portion were identified in solar salt. In doenjang, Candida versatilis were predominantly detected (92.62%). Non-halophilic mold were dominantly identified from meju (low-salt fermented soybean), while halophilic bacteria and archaea for solar salt and salt-tolerance fungi such as C. versatilis for doenjang (high-salt fermented soybean) were frequently detected. These results implied that most predominant fungal species might not be migrated from meju and/or solar salt into doenjang.

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7메주 발효 관련 Bacillus, Enterococcus, Staphylococcus 속 우점종 확인

저자 : 장미현 ( Mihyun Jang ) , 정도원 ( Do-won Jeong ) , 이종훈 ( Jong-hoon Lee )

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 359-363 (5 pages)

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국내 5개 지역에서 수집한 12개 메주로부터 Bacillus, Enterococcus, Staphylococcus 속 bacteria를 선택배지를 이용하여 분리하고, 16S ribosomal RNA 유전자 및 gmk (guanylate kinase) 유전자 염기서열분석을 통해 동정하여, 이들 속의 우점종을 검토하였다. Bacillus 속과 Enterococcus 속은 모든 시료로부터 검출되었고, Bacillus 속은 11개 시료에서 총균수 대비 15% 이상 검출되어 메주 발효에서 가장 우점하는 bacteria로 확인되었다. Staphylococcus 속은 6개 시료에서만 검출되었다. Bacillus 속으로 동정된 151개 분리 주는 B. velezensis, B. sonorensis 순으로 우점하였고, B. subtilis, B. licheniformis가 그 뒤를 이었다. Enterococcus 속으로 동정된 165개 분리주 중, 163균주가 E. faecium으로 확인되었다. Staphylococcus 속으로 동정된 82개 분리주에는 6종의 coagulase-negative Staphylococcus 존재가 확인 되었고, S. xylosus가 우점종으로 확인되었다.


Meju is a spontaneously fermented soybean product produced in Korea. We isolated the bacteria of the genera Bacillus, Enterococcus, and Staphylococcus from 12 meju samples collected from five regions of Korea using selective media, and we identified them based on the sequence analysis of 16S ribosomal RNA and gmk (guanylate kinase) genes. Bacillus and Enterococcus strains were identified from all samples and the numbers of Bacillus strains in the 11 samples were >15% of the total cell number. Therefore, the genus Bacillus was confirmed as the predominant bacterial group of meju. Staphylococcus strains were identified from six samples. The identified 151 Bacillus isolates predominated in the following order: B. velezensis, B. sonorensis, B. subtilis, and B. licheniformis. Among the 165 Enterococcus isolates, 163 strains were identified as E. faecium. Eighty-two Staphylococcus isolates were classified into six species of coagulase-negative Staphylococcus group and S. xylosus was the predominant species.

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8Characterization of Alpha-Amylase from Aspergillus niger Aggregate F Isolated from a Fermented Cassava Gatot Grown in Potato Peel Waste Medium

저자 : Cindy Angelia , Astia Sanjaya , Aida , Ellen Tanudjaja , Hans Victor , Antari Daru Cahyani , Tjie Jan Tan , Reinhard Pinontoan

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 364-371 (8 pages)

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The use of GRAS microorganisms isolated from fermented foods during amylase production using an economical food-waste medium provides more opportunities to produce amylase with a wider range of applications. Hence, this study aimed to isolate a good amylase-producing fungi from the traditional Indonesian fermented cassava, gatot, and to identify the amylase-producing capability of the isolate in a potato peel waste (PPW) medium. Black-colored fungi isolated from gatot was morphologically identified and the amylase produced was characterized using SDS-PAGE and Native PAGE. The isolate was then grown on PPW medium, and the amylase produced was further characterized. Morphological identification and enzyme characterization revealed that the Aspergillus niger aggregate F isolated from gatot secreted an active extracellular α-amylase with an optimum pH of 5-6. In conclusion, Aspergillus niger aggregate F isolated from gatot can be used to produce α-amylase using PPW as a medium.

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9Microwave Radiation Effects on the Process of Escherichia coli Cultivation

저자 : Denis Kuznetsov , Igor Volkhin , Ekaterina Orlova , Valery Neschislyaev , Alevtina Balandina , Anna Shirokikh

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 372-380 (9 pages)

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Modern biotechnological industries have been attempting to improve the efficiency of bacterial strain cultivation. Millimeter wave electromagnetic radiation can have a varied influence on E. coli cultivation processes. The results of the study revealed that when a microwave radiation of low intensity is applied to positively adjust the conditions for the accumulation of bacterial culture biomass, a significant role is played not only by radiation parameters, but also by concomitant biological factors, which influence the reproducibility of the cultivation process and help obtain a useful biotechnological effect. The authors suggest a model that can be used to study the molecular mechanisms underlying the changes in the buildup of E. coli biomass under the influence of electromagnetic radiation.

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10Identification of Green Alga Chlorella vulgaris Isolated from Freshwater and Improvement Biodiesel Productivity via UV Irradiation

저자 : Mohamed A. Gomaa , Mohamed H. Refaat , Tamer M. Salim , Abo El-khair B. El-sayed , Makhlouf M. Bekhit

발행기관 : 한국미생물생명공학회(구 한국산업미생물학회) 간행물 : 한국미생물·생명공학회지 47권 3호 발행 연도 : 2019 페이지 : pp. 381-389 (9 pages)

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Chlorella vulgaris was isolated from the Nile River, Qalubia Governorate, Egypt, for possible use in biodiesel production. BG-II nutrient growth media was used for isolation and laboratory growth. Identification was performed via 18S rRNA gene amplification, followed by sequencing. The alga was exposed to UVC (254 nm) for 15, 30, and 45 s to improve dry weight accumulation and to increase the oil production. Daily measurements of dry weight (g·l-1) were performed; oil content and volumetric lipid productivity were also determined. UV-C exposure led to an increase in the volumetric lipid productivity by 27, 27.3, and 32.4 mg·l-1·d-1 with 15, 30, and 45 s, respectively, as compared with the control, which resulted in 18 mg·l-1·d-1. Of the examined mutants, the one with the highest productivity was re-irradiated by UV-C (254 nm) for 15, 30, 45, and 60 s. For 15 s of exposure time, the oil content increased to 34%, while it was 31% at 30 s; further, it decreased to 22% at 45 and 60 s exposures. The fatty acid methyl ester profile was 82.22% in the first mutant at 45 s, compared with the wild strain that contained a total of 66.01% of FAs. Furthermore, the highest levels of polyunsaturated fatty acid methyl ester were observed in the mutant exposed for 45 s, and it reached 11.41%, which reduced the cetane number to 71.3.

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