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Jouranl of Fish Pathology

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수록정보
수록범위 : 1권1호(1988)~33권1호(2020) |수록논문 수 : 791
한국어병학회지
33권1호(2020년 06월) 수록논문
최근 권호 논문
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1중국에서 수입된 잉어 및 붕어의 잉어부종바이러스병 및 허피스바이러스성 조혈기괴사증 모니터링

저자 : 권세련 ( Se Ryun Kwon )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 1-6 (6 pages)

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In this study, carp edemavirus disease and herpesviral hematopoietic necrosis were examined in carp (Cyprinus carpio) and crucian carp (Carassius auratus) imported from China. From August 2016 to May 2017, 100 carp and 300 crucian carp were examined. There were no clinical symptoms in all the tested fish, and no internal or external parasitic infections were identified except for the detection of Argulus sp. in one leather carp. Carp edema virus disease was examined by PCR in C. carpio, but all were negative. In C. auratus, herpesviral hematopoietic necrosis was tested by PCR and 4 out of 300 were positive. The results of this study are expected to be useful as basic data for conducting risk analysis on the relevant organisms and counterpart countries or for designating new pathogens for quarantine.

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2넙치(Paralichthys olivaceus) 구강에 기생하는 Neoheterobothrium hirame의 형태 및 감염어의 병리조직

저자 : 이범석 ( Beom-seok Lee ) , 김정호 ( Jeong-ho Kim ) , 김태호 ( Taeho Kim ) , 정성주 ( Sung-ju Jung )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 7-14 (8 pages)

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실험실에서 순환여과시스템으로 5-6개월 사육하던 넙치 치어에서 아가미의 심한 빈혈을 나타내는 질병이 발생하였다. 현미경관찰에서 아가미에서는 유충이, 입천정에서는 성숙한 성충이 관찰되었다. 성충의 체장은 5.60-9.32 mm이며 방추형 몸의 길이는 3.83-6.32 mm, 부착기는 방추형 몸에서부터 연결된 isthmus의 끝부분에 꽃자루 모양으로 존재하였고 부착기의 끝에는 8개의 파악기가 있었다. 충의 형태학적 관찰로부터 단생류에 속하는 Neoheterobothirum hirame로 동정하였다. 감염어의 적혈구용적는 평균 10.3±2.8%로 정상 넙치의 31.4±4.2%보다 현저히 낮았다. 병리조직학적으로 N. hirame에 감염 된 넙치는 아가미 새판과 새엽을 흐르는 혈관내의 적혈구가 감소하고 비장의 적수(red pulp) 영역이 감소되고 간세포는 위축이 관찰되었다. 이상의 결과로부터 넙치에 심한 빈혈을 일으킨 원인은 N. hirame인 것을 밝혔다. 이 연구는 순환여과식 사육시스템에서는 사육수의 교환이 적으므로 소독장치가 충분하지 못할 경우 병원체의 증식이 문제가 될 수 있는 예를 보이고 있다.


A disease that manifested severe anemia of the gills occurred in the olive flounder (Paralichthys olivaceus), which was cultured for 5-6 months with a recirculation water system in the laboratory. Microscopic observations showed immature parasite in the gills and mature adults in the oral cavity. The matured parasite was 5.60-9.32 (7.42) mm in total length, with 4 pairs of clamps on the pedunculated haptor, which was separated from the body proper by a long isthmus. From the morphological observations of the larvae, it was identified as Neoheterobothirum hirame belonging to the monogenea. The average hematocrite of infected fish was 10.3 ± 2.8%, significantly lower than that of normal flounder 31.4 ± 4.2%. Histopathologically, fish infected with N. hirame exhibited reduced numbers of erythrocytes in blood vessels of the gill filament and lamellae, reduced red pulp area of the spleen, and hepatocyte atrophy. From the above results, the cause of severe anemia in olive flounder was identified as N. hirame infection. This study shows an example where the growth of pathogens can be a problem in the recirculation system due to low water exchange rate.

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3Neobenedenia girellae infection of aquarium-raised snubnose pompano (Trachinotus blochii) in Korea

저자 : U-hwa Nam , Hyun-joon Seo , Ilson Hwang , Jeong-ho Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 15-21 (7 pages)

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We found skin flukes in snubnose pompano (Trachinotus blochii) from a public aquarium and attempted clear identification of them to the species level by morphology and molecular analyses. Skin flukes were collected from snubnose pompano showing dyspnea, anorexia and mild hemorrhage on the skin. All the fish samples (n=2) were infected with the flukes on the skin, gill and eyes, covered with excessive mucus. The isolated worms were transferred for making slide specimen and PCR amplification targeting 18S rDNA, 28S rDNA, mitochondrial cytochrome c oxidase subunit 1 (mt cox1) and cytochrome b (Cytb) genes for further analyses. Morphology and measurements data of our slide specimen coincided with those of Neobenedenia girellae. The sequence data of 2 genes (28S rDNA and Cytb) and the phylogenetic trees revealed that our specimen consistently belonged to the N. girellae clade. For 18S rDNA and mt cox1 genes, there was no sequence of either of these 2 Neobenedenia species from the type host available in GenBank. This is the first record of N. girellae in snubnose pompano, but it is still unclear if the snubnose pompano is a natural host for N. girellae or not because N. girellae is known to have an unusual broad host range and the host-switching can occur particularly in captive conditions such as aquarium or aquaculture facilities.

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4Changes in hematoserological profiles and leukocyte redistribution in rainbow trout (Oncorhynchus mykiss) under progressive hypoxia

저자 : Hyeongjin Roh , Bo Seong Kim , Ahran Kim , Nameun Kim , Mu Kun Lee , Chan-il Park , Do-hyung Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 23-34 (12 pages)

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In recent years, global warming is causing dramatic environmental changes and deterioration, such as hypoxia, leading to reduced survival rate and growth performance of farmed aquatic animals. Hence, understanding systemic immuno-physiological changes in fish under environmental stress might be important to maximize aquaculture production. In this study, we investigated physiological changes in rainbow trout exposed to hypoxic stress by monitoring changes in blood chemistry, leukocyte population, and expression levels of related cytokine genes. Hematological and serological factors were evaluated in blood obtained from rainbow trout sampled at a dissolved level of 4.6 mg O2 L-1 and 2.1 mg O2 L-1. Blood and head kidney tissue obtained at each sampling time point were used to determine erythrocyte size, leukocyte population, and cytokine gene expression. The level of LDH and GPT in fish under progressive hypoxia were significantly increased in plasma. Likewise, the (Granulocyte + Macrophage)/lymphocyte ratio (%) of fish exposed to hypoxia was significantly lower than that in fish in the control group. Such changes might be due to the rapid movement of lymphocytes in fish exposed to acute hypoxia. In this study, significant up-regulation in expression levels of IL-1β and IL-6 gene appeared to be involved in the redistribution of leukocytes in rainbow trout. This is the first study to demonstrate the involvement of cytokines in leukocyte trafficking in fish exposed to hypoxia. It will help us understand systemic physiological changes and mechanisms involved in teleost under hypoxic stress.

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5Effects of hypoxia on the concentration of circulating miR-210 in serum and the expression of HIF-1α and HSP90α in tissues of olive flounder (Paralichthys olivaceus)

저자 : Najib Abdellaoui , Jun Soung Kwak , Ki Hong Kim

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 35-43 (9 pages)

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Hypoxia is a serious problem in the marine ecosystem causing a decline in aquatic resources. MicroRNAs (miRNAs) regulate the expression of genes through binding to the corresponding sequences of their target mRNAs. Especially, miRNAs in the cytoplasm can be secreted into body fluids, which called circulating miRNAs, and the availability of circulating miRNAs as biomarkers for hypoxia has been demonstrated in mammals. However, there has been no report on the hypoxiamediated changes in the circulating miRNAs in fish. miR-210 is known as the representative hypoxia- responsive circulating miRNA in mammals. To know whether fish miR-210 also respond to hypoxia, we analyzed the change of circulating miR-210 quantity in the serum of olive flounder (Paralichthys olivaceus) in response to hypoxia. The expression of hypoxia related genes, hypoxia inducible factor 1α (HIF-1α) and the heat shock protein 90α (HSP90α) was also analyzed. Similar to the reports from mammals, miR-210-5p and miR-210-3p were significantly increased in the serum of olive flounder in response to hypoxia, suggesting that circulating miR-210 levels in the serum can be used as a noninvasive prognostic biomarker for fish suffered hypoxia. The target genes of miR-210 were related to various biological processes, which explains the major regulatory role of miR-210 in response to hypoxia. The expression of HIF-1α and HSP90α in the tissues was also up-regulated by hypoxia. Considering the critical role of HIF-1α in miR-210 expression and HSP90 in miRNAs function, the present up-regulation of HIF-1α and HSP90α might be related to the increase of circulatory miR-210, and the interaction mechanism among HIF-1α, HSP90α, and hypoxia-responsive microRNAs in fish should be further studied.

KCI후보

6흰다리새우(Litopenaeus vannamei)에서 광범위 반합성 항생제 doxycycline의 독성연구

저자 : 이채원 ( Chae Won Lee ) , 배준성 ( Jun Sung Bae ) , 양찬영 ( Chan Yeong Yang ) , 정은하 ( Eun Ha Jeoung ) , 이지훈 ( Ji-hoon Lee ) , 박관하 ( Kwan Ha Park )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 45-53 (9 pages)

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Doxycycline is a semi-synthetic broad-spectrum antibiotic, and it has been used to get rid of bacteria in animals and humans. The use of antibiotics has greatly contributed to the aquaculture production although its misuse sometimes presents public health problems. This study was performed to investigate the toxic effects of doxycycline on whiteleg shrimp (Litopenaeus vannamei) administered for possible infection treatments. The shrimp were allocated into four groups and doxycycline was fed three times a day for 7 days at 0, 20, 50 and 100 mg/kg to each group. After 24 hr following the 7-day treatment, hemolymph and hepatopancreas were used for blood and biochemical analysis: Total hemocyte counts, Total protein, Total cholesterol, Gluscose, Glutamic pyruvic transaminase, Glutamic oxaloacetic transaminase, Glutathione peroxidase, Superoxide dismutase, Glutathione-s-transferases, Total antioxidant capacity colorimetric and Acid phosphatase. In addition, histopathological examination was performed on the hepatopancreas and muscle. It was observed that body weight gain was significantly retarded in 100 mg/kg doxycycline group. Doxycycline was found to induce biochemical or functional disorders at 100 mg/kg as observed many of the blood and biochemical parameters were significantly reduced. In conclusion, it was judged that there will be no major toxicity problems with doxycycline when used for shrimp aquaculture at regular doses.

KCI후보

7톨트라주릴(Toltrazuril)을 이용한 제주도 넙치(Paralichthys olivaceus)의 점액포자충성 여윔증에 대한 치료법 연구

저자 : 강미래 ( Mi-rae Kang ) , 김예지 ( Ye-ji Kim ) , 전려진 ( Lyu Jin Jun ) , 김승민 ( Seung Min Kim ) , 김성현 ( Sung-hyun Kim ) , 한소리 ( Sori Han ) , 정준범 ( Joon Bum Jeong )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 55-62 (8 pages)

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본 연구에서는 점액포자충에 의한 넙치의 여윔증 치료 후보물질을 탐색하기 위하여, Coxi-stop, Coxiclin 및 BLEAN80 등의 제품을 사용하여 in vitro 및 in vivo 실험을 실시하였다. 톨트라주릴이 주성분인 Coxi-stop의 경우, BF-2 cell을 사용한 in vitro 실험에서 점액포자충의 활성을 감소시키는 결과를 보였고, cell lysis와 같은 세포 독성 현상이 나타나지 않았다. In vivo 실험에서 Coxi-stop을 사용하여 약욕한 실험군은 대조군에 비하여 누적폐사율이 낮게 나타났으며, 이것은 톨트라주릴이 어류 기생충성 질병에 치료 효과가 있다는 기존의 보고와 유사한 결과이다. 본 연구에서는 톨트라주릴이 넙치의 여윔증 치료제로서 가능성이 있는 후보 물질이라는 것을 제시한다.


In order to search candidates for the treatment of myxosporean emaciation disease of olive flounder, Paralichthys olivaceus, in vitro and in vivo experiments were conducted using products such as Coxi-stop, Coxiclin, and BLEAN80. In the case of Coxi-stop, whose major component is toltrazuril, in vitro experiment using BF-2 cells showed a tendency of reducing the activity of myxosporea and did not exhibit cytotoxicity such as cell lysis. In the in vivo activity measurements, the experimental group immersed with Coxi-stop showed a lower cumulative mortality rate than the control group, this result is similar to the previous report that toltrazuril has a therapeutic effect on parasitic fish disease. This study suggests that toltrazuril is a potential candidate for the treatment of emaciation disease of olive flounder.

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8코로나 방전 플라즈마 처리수에 의한 어류 병원체 소독 효과

저자 : 유진호 ( Jin Ho You ) , 이지현 ( Ji Hyun Lee ) , 문성희 ( Seong Hee Mun ) , 권세련 ( Se Ryun Kwon ) , 박태섭 ( Tae Sup Park ) , 권준영 ( Joon Yeong Kwon )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 63-69 (7 pages)

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Fish culture is constantly threatened by various infectious diseases which are largely transmitted by water. Plasma technology is being used to sterilize polluted water in many industries. In this study, two bacterial pathogens Aeromonas salmonicida and Streptococcus iniae, and a virus (viral hemorrhagic septicemia virus, VHSV) were subjected to plasma water that was produced by a corona discharge system. Growth of A. salmonicida was greatly inhibited from 105.61 CFU/ml in positive control to 103.51 CFU/ml in treated group by only 60 sec contact with plasma water. Similarly, S. iniae was inhibited from 105.85 CFU/ml to 103.40 CFU/ml. VHSV titer also decreased from 104.1 TCID50/ ml to 101.45 TCID50/ml by the same treatment. Activation of water by the plasma was confirmed by the existence of ozone in the plasma water. These results suggest that plasma water could efficiently disinfect fish pathogens, possibly by the action of reactive oxygen species contained in the plasma water.

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92018-2019년 양식산 흰다리새우의 바이러스 (IHHNV, TSV, IMNV, YHV, CMNV) 모니터링

저자 : 샴코카뚜니발띨 ( Shyam Kokkattunivarthil ) , 김위식 ( Wi-sik Kim )

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 71-75 (5 pages)

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A survey was conducted to investigate viral infections in 184 whiteleg shrimp (Litopenaeus vannamei) collected from nine farms and one wholesale fish vendor during 2018 and 2019. Gill and abdominal muscle of shrimp were tested for the presence of five viruses, viz. infectious hypodermal and haematopoietic necrosis virus, taura syndrome virus, infectious myonecrosis virus, yellow head virus genotype 1, and covert mortality nodavirus by reverse transcription-polymerase chain reaction (RTPCR) and PCR. These viruses were not detected in any of 184 samples, screened under the study.

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10국내 양식 흰다리새우에서 검출된 노랑머리바이러스(YHV-8)의 유전학적 특성

저자 : 김석렬 , Gunasekara Cwr , Wimalasena Shmp , 신기욱

발행기관 : 한국어병학회 간행물 : 한국어병학회지 33권 1호 발행 연도 : 2020 페이지 : pp. 77-81 (5 pages)

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This study was performed for screening yellow head virus(YHV) complex in 252 including 235 white leg shrimps (Litopenaeus vannamei) and 17 oriental shrimp (Fenneropenaeus chinensis) collected from 18 farms located in southwestern province of Korea. The virus complex was detected by nested reverse-transcriptase polymerase chain reaction (RT-PCR) assay. In the assay, amplicons were resulted in RNAs exracted from 38 shrimps (21 white leg shrimps and 17 oriental shrimps) obtained from 7 farms. In phylogenetic analysis using sequences of ORF1b gene, all 38 sequences obtained in this study formed an independent lineage with YHV-8 genotype firstly isolated in China, belonged to an YHV-8 clade.

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1연안해역에서 석유오염물질의 세균학적 분해에 관한 연구

(2006)홍길동 외 1명심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동심리학41회 피인용

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2미국의 비트코인 규제

(2006)홍길동41회 피인용

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