간행물

한국응용생명화학회(구 한국농화학회)> 한국응용생명화학회지(구 한국농화학회지)

한국응용생명화학회지(구 한국농화학회지)

Applied Biological Chemistry

  • : 한국응용생명화학회(구 한국농화학회)
  • : 농학분야  >  농화학
  • : KCI등재
  • : SCI
  • : 연속간행물
  • : 격월
  • : 2468-0834
  • : 2468-0842
  • : 한국농화학회지(~2003)→한국응용생명화학회지(2004~)

수록정보
수록범위 : 1권0호(1960)~54권6호(2011) |수록논문 수 : 3,057
한국응용생명화학회지(구 한국농화학회지)
54권6호(2011년 12월) 수록논문
최근 권호 논문
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KCI등재 SCI

1Modification of Isoflavones by Processing and Photosensitization in Model and Food Systems

저자 : Mi Ja Kim , Jae Hwan Lee

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 833-840 (8 pages)

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Isoflavones are major phenolic compounds found in soybeans and soy-based foods. Healthbeneficial functions of soy foods are closely associated with these phytoestrogenic compounds. Twelve chemical forms of isoflavones are found in soybeans including three aglycones (genistein, daidzein, and glycitein), and their corresponding conjugated β-glucosides, 6``-O-acetyl-β- glucosides, or 6``-O-malonyl-β-glucosides. Distributions of isoflavones in soy foods are dependent on the processing conditions especially the applied thermal energy and moisture contents. Conventional thermal treatment with high moisture and at temperature lower than 100, such as boiling and cooking, transforms 6``-O-malonyl-β-derivatives into β-glucosides via intra-conversion. Dry heat treatment with limited moisture contents such as frying, toasting, baking, explosive puffing, and roasting process increases the formation of 6``-O-acetyl-β-derivatives of isoflavones through decarboxylation from 6``-O-malonyl-β-derivatives. Fermentation with microorganisms or natural products containing high β-glucosidase activity can produce aglycones from β-glucosides in soy foods. New isoflavone metabolites such as 6``-O-succinyl daidzin and 6``-O-succinyl genistin are found in soy-fermented foods with Bacillus species. Major precursors for the generation of these succinyl isoflavones in Bacillus-fermented soy foods are β-glucosides rather than aglycones. Riboflavin photosensitization can decrease the stability of isoflavones greatly in model systems and soymilk, which implies that isoflavones in soy foods require proper protection from light exposure to maintain its functional properties in foods. This review was aimed to provide comprehensive understanding on the transformation of isoflavones in soy foods treated with diverse processing methods including thermal treatments, fermentation using natural compounds, microorganisms, and photosensitization.

KCI등재 SCI

2Vitamin D and Breast Cancer: Molecular Communications

저자 : Hong Jin Lee

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 841-851 (11 pages)

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Epidemiological studies have demonstrated that vitamin D status is inversely associated with breast cancer incidence, mortality, and recurrences, suggesting vitamin D as a potent agent to reduce the risk of breast cancer. The hormonally active metabolite of vitamin D, 1α,25- dihydroxyvitamin D (1α,25(OH)2D, calcitriol), and its analogs have exerted inhibitory activity of cellular proliferation through arresting cell cycle and inducing apoptosis, and suppressive effects on the invasion, angiogenesis, and metastasis of breast cancer. In the studies of molecular basis of vitamin D activities, many upstream signaling pathways cross-talking with vitamin D signaling have been investigated. 1α,25(OH)2D and its analogs regulates different signaling pathways mediated by transforming growth factor-β superfamily, epidermal growth factor receptors family, estrogen signaling-related molecules, insulin-like growth factor-binding proteins, and protein kinase C. The multipotent activities of vitamin D in signaling modulation may be efficient and effective in suppressing highly heterogeneous breast cancer.

KCI등재 SCI

3Proteomic Analysis of Hepatotoxicity Induced by Titanium Nanoparticles in Mouse Liver

저자 : Yu Mi Jeon , Seul Ki Park , Mi Young Lee

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 852-859 (8 pages)

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Differentially expressed proteins in mouse liver caused by toxicity of titanium nanoparticles (TiO2 NPs) were screened. More than 1,400 protein spots in mouse liver were detected by twodimensional gel electrophoresis, and 15 proteins that showed greater than 2-fold expressional changes in response to TiO2 NPs were identified by liquid chromatography-tandem mass spectrometry. Of these, 12 proteins were down-regulated and 3 proteins were up-regulated upon treatment with TiO2 NPs. The 15 differentially expressed proteins could be used for detection of inflammation, apoptosis, and antioxidative reaction for treatment of acute hepatic damage by TiO2 NPs.

KCI등재 SCI

4Application of Polymerase Chain Reaction with Disposable Amplicon Detection Device for Identification of Regulatory Gene Introduced into Genetically Modified Maize

저자 : Hee Jong Woo , Chan Mi Chung , Kong Sik Shin , Myung Ho Lim , Ki Jong Lee , Yong Gu Cho , Soon Jong Kweon , Seok Cheol Suh

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 860-864 (5 pages)

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A novel genetically modified organism (GMO) detection method was developed using a simple and rapid procedure to identify a regulatory gene introduced into genetically modified (GM) maize. After DNA extraction from GM maize flours, fragments of the conserved sequence of the cauliflower masaic virus (CaMV) 35S promoter gene along with a competitive internal control gene were amplified using duplex polymerase chain reaction (PCR). Amplification products were then detected using a disposable detection device. The quantitative detection limit for GM maize 59122 was found to be 1.0% (w/w). Because the combination-method involving PCR technology coupled with a disposable detection device does not require expensive instrumentation or expertise, it will serve as a valuable alternative to immunoassays and traditional PCR-based tests in the detection of GMOs.

KCI등재 SCI

5A New Flavonolignan from the Aerial Parts of Oryza sativa L. Inhibits Nitric oxide Production in RAW 264.7 Macrophage Cells

저자 : Rak Hun Jeong , Dae Young Lee , Jin Gyeong Cho , Sang Min Lee , Hee Cheol Kang , Woo Duck Seo , Hwang Won Kang , Ji Young Kim , Nam In Baek

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 865-870 (6 pages)

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Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned using n-hexane, ethyl acetate (EtOAc), nbutanol, and H2O. From the EtOAc fraction, a new flavonolignan (2) and a known flavonolignan, salcolin B (1), were isolated through repeated SiO2 and octadecyl silica gel column chromatography. Based on NMR, mass spectrometer, and IR spectroscopic data, the chemical structure of compound (2) was determined to be tricin-4`-O-[erythro-β-guaiacyl-(7``-O-methyl)- glyceryl] ether, which has thus far never been reported and was named salcolin C (2). Salcolin B (1) and salcolin C (2) dose-dependently inhibited nitric oxide production in RAW 264.7 cells with IC50 values of 29.8±0.6 and 10.8±1.0 μM, respectively, without visible toxic effect.

KCI등재 SCI

6Characterization of Factors Favoring the Expression and Purification of Recombinant LL-37 from Escherichia coli

저자 : Ja Young Moon , Dae Ook Kang , Yong Kweon Cho , Kwang Hoon Kong , Dong Kuk Lee , Ayyalusamy Ramamoorthy

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 871-880 (10 pages)

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The only human antimicrobial peptide, LL-37, was overexpressed in soluble form using Escherichia coli-based expression system. Recombinant LL-37 production in E. coli was optimized for use in large quantities for studying antimicrobial activity against Helicobacter pylori strains. We previously reported a method to express and purify LL-37 using Glutathione S-transferase (GST) fusion system. Herein presents method suitable for producing recombinant LL-37 from the recombinant GST-LL-37 fusion protein by recovering from both soluble protein fractions and inclusion bodies. Compared to the yield reported previously, the yield of recombinant GST-LL-37 protein was much improved to 10 mg/L of culture by affinity chromatography using GSTrap FF (1 mL) affinity chromatography column. These results suggest that the production method used in present study is useful in obtaining large quantity of recombinant LL-37 peptide. The optimized recovery protocol from inclusion bodies was highly contributable in raising yield of GST-LL-37 fusion protein. When treated with Factor Xa, GST-LL-37 fusion protein recovered from both soluble protein fractions and inclusion bodies released an approximate 4.5-kDa protein, which was the expected size of LL-37; GST-LL-37 fusion protein recovered from both soluble protein fractions and inclusion bodies was also confirmed by enzymatic digestion with thrombin, which produced LL-37 peptide containing six extra amino acid residues, Gly-Ile-Ile-Glu-Gly-Arg, on Nterminus of LL-37. Purified recombinant LL-37 showed nearly identical antimicrobial activities against H. pylori strains as that of synthetic LL-37, suggesting its application to functional study with therapeutic potential on gastric pathogen.

KCI등재 SCI

7Piperidine Azasugars Displaying Competitive α-Rhamnosidase Inhibition and their Kinetic Mechanism

저자 : Ki Hun Park , Ji Won Lee , Jin Hyo Kim , Jung Keun Cho , Rajesh Rengasamy , Marcus John Curtis Long

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 881-888 (8 pages)

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Azasugars derived from L-alanine and L-serine were screened for inhibitory activity against α- rhamnosidase. The enantiomers of 1,6-dideoxynojirimycin (ent-1,6-dDNJ) (1) and (2S,3R)-2- (hydroxymethyl)piperidin-3-ol (5) showed highly specific and potent inhibition against α- rhamnosidase with K i values of 4.2 and 16.6 μM, respectively. Structure of the best inhibitor features the same stereochemical configuration as L-rhamnose at C2, C3, and C4 centers. In kinetic studies, both compounds exhibited competitive inhibition behavior. Compound 1 manifested simple reversible slow-binding inhibition with the following kinetic parameters: k 3= 1.17 nM·1 min·1, k 4=5.96 ×10·3 min·1, and K i app=5.1 mM.

KCI등재 SCI

8Sublethal Effects of Flonicamid and Thiamethoxam on Green Peach Aphid, Myzus persicae and Feeding Behavior Analysis

저자 : Sun Ran Cho , Hyun Na Koo , Chang Mann Yoon , Gil Hah Kim

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 889-898 (10 pages)

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The green peach aphid, Myzus persicae Sulzer, is an important sap-sucking pest of many plants, including Chinese cabbage. The objective of the present study was to determine the effects of sublethal concentrations of two insecticides (flonicamid and thiamethoxam) and the action mechanisms on the feeding behavior of M. persicae. The median lethal concentrations (LC50) of flonicamid and thiamethoxam for adult M. persicae were 2.56 and 4.02 mg/L, respectively. The sublethal concentrations of flonicamid were 0.44 mg/L (LC10) and 1.25 mg/L (LC30), and those of thiamethoxam were 1.19 mg/L (LC10) and 2.45 mg/L (LC30). The developmental period of M. persicae nymphs was 5.9 days at LC10 and 6.1 days at LC30 for both insecticides compared to 5.7 days for the control. Adult longevities at LC10 and LC30 of flonicamid were 13.2 and 13.7 days, respectively. Adult longevity at LC10 of thiamethoxam was 14.7 days. Control adult longevity was 11.6 days. Total fecundity was higher at LC10 (41.8 offspring/female) and LC30 (43.0 offspring/ female) of flonicamid, and at LC10 (42.1 offspring/female) of thiamethoxam than that of the control (29.5 offspring/female). Feeding behavior analysis using an electrical penetration graph showed that sublethal doses of flonicamid and thiamethoxam had significant effects on the duration of phloem ingestion. However, higher doses of flonicamid induced starvation by inhibition of phloem ingestion and higher doses of thiamethoxam induced contact toxicity rather than inhibition of feeding behavior. This study provides the basis for a more efficient use of these pesticides in Korea.

KCI등재 SCI

9Influence of Chinese Milkvetch (Astragalus sinicus L.) with No-tillage on Soil Biotic Factors and Rice Yield

저자 : Min Keun Kim , Young Han Lee , Tae Ho Kang , Han Dae Yun

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 899-909 (11 pages)

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The bacterial community, biotic factors, and rice yield of Chinese Milkvetch (Astragalus sinicus L.) with no-tillage and conventional chemical fertilizer treatment were evaluated. Analysis of bacterial isolates from each treatment plots with 16S rDNA sequences revealed 486 isolates from 18 different bacterial species. The frequency of spore-forming bacteria, including Bacillus sp. and some Actinomycetes appeared to be high under low temperature conditions, whereas Proteobacteria disappeared at low temperature. Most extracellular hydrolytic enzyme activity showing bacteria belong to low G + C Gram(+) bacteria (LGCGPB) compared to high G + C Gram(-) bacteria (HGCGPB) and Proteobacteria. In addition, the total soil carbon content in Chinese Milkvetch treated soil was higher than that of conventional tilled soil samples. Rice yield was slightly higher in the conventionaltilled field (4.13±0.08 Mg/ha) compared to the Chinese Milkvetch-treated field (3.94±0.15 Mg/ha). However, the field treated with Chinese Milkvetch followed by no-tillage was effective in sustaining LGCGP bacterial species that promote biogeochemical cycling and enrich microbial biomass on paddy soil (3.94±0.15 Mg/ha). These results showed that the Chinese Milkvetch treatment with no-tillage is effective in conserving the soil environment without significant yield loss.

KCI등재 SCI

10Assessment of Copper and Zinc in Soils and Fruit with the Age of an Apple Orchard

저자 : Bong Ju Park , Jae Young Cho

발행기관 : 한국응용생명화학회(구 한국농화학회) 간행물 : 한국응용생명화학회지(구 한국농화학회지) 54권 6호 발행 연도 : 2011 페이지 : pp. 910-914 (5 pages)

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Chemical fractions of copper and zinc in soils were evaluated and, the transfer coefficient of copper and zinc from soil to apples depending on age of apple orchard were determined. The fractions of copper and zinc in surface soil were in decreasing order: residual > organic-bound > Fe-Mn oxides > carbonates and exchangeable > water-soluble fractions. With increasing age of apple orchard, the residual fraction showed the highest residual fractions of copper and zinc, whereas those of water soluble and exchangeable fractions were relatively low. Tc values of copper and zinc did not show significant difference with the age of apple orchard (p >0.05). As our results show that the concentrations of Zn and Cu in soil increase with age of apple orchard, continuous monitoring and pollution control of hazardous material are needed to prevent the excessive build-up of heavy metals in the food chain.

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