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한국약용작물학회> 한국약용작물학회지

한국약용작물학회지

Korean Journal of Medicinal Crop Science

  • : 한국약용작물학회
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수록정보
수록범위 : 1권1호(1993)~25권6호(2017) |수록논문 수 : 1,435
한국약용작물학회지
25권6호(2017년 12월) 수록논문
최근 권호 논문
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KCI등재

1트리메틸틴 처리로 유도된 기억·학습 능력 손상 모델에 대한 계피와 금앵자 혼합추출물의 개선 효과

저자 : 최수정 ( Soo Jung Choi ) , 김초롱 ( Cho Rong Kim ) , 박찬규 ( Chan Kyu Park ) , 김민철 ( Min Chul Gim ) , 최종헌 ( Jong Hun Choi ) , 신동훈 ( Dong Hoon Shin )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 353-360 (8 pages)

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Background: A critical features of Alzheimer's disease (AD) is cognitive dysfunction, which partly arises from decreased in acetylcholine levels. AD afftected brains are characterized by extensive oxidative stress, which is thought to be primarily induced by the amyloid beta (Aβ) peptide. In a previous study, Cinnamomum loureiroi tincture inhibited acetylcholinesterase (AchE) activity. That study identified AChE inhibitor in the C. loureiroi extract. Furthermore, the C. loureiroi extract enhanced memory in a trimethyltin (TMT)-induced model of cognitive dysfunction, as assessed via two behavioral tests. Rosa laevigata extract protected against oxidative stress-induced cytotoxicity. Administrating R. laevigata extracts to mice significantly reversed Aβ-induced learning and memory impairment, as shown in behavioral tests.
Methods and Results: We conducted behavioral to examine the synergistic effects of C. loureiroi and R. laevigata extracts in inhibiting AChE and counteracting TMT-induced learning and memory losses. We also performed biochemical assays. The biochemical results showed a relationship between increased oxidative stress and cholinergic neurons damage in TMT-treated mice.
Conclusions: A diet containing C. loureiroi and R. laevigata extracts ameliorated learning and memory impairments in the Y-maze and passive avoidance tests, and exerted synergistic inhibitory effect against AChE and lipid peroxidation.

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2엽록체기반 SSR marker를 이용한 당귀의 기원 판별

저자 : 박상익 ( Sang Ik Park ) , 황보경 ( Kyeong Hwangbo ) , 길진수 ( Jinsu Gil ) , 정희 ( Hee Chung ) , 김호방 ( Ho Bang Kim ) , 김옥태 ( Ok Tae Kim ) , 김성철 ( Seong Cheol Kim ) , 구성철 ( Sung Cheol Koo ) , 엄유리 ( Yurry Um ) , 이이 ( Yi Lee )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 361-366 (6 pages)

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Background: In the herbal medicinal industry, Angelica gigas Nakai, Angelica sinensis (Oliv.) Diels. and Angelica acutiloba (Siebold & Zucc.) Kitag. are often confused, because the roots of the three species can not be distinguished by their appearance. This confusion can cause serious side effects. In this study, we determined the origins of Angelica roots distributed in the Korean market using the simple sequence repeat (SSR) markers developed based on the A. gigas chloroplast DNA sequence.
Methods and Results: We collected twenty seven A. gigas and three A. acutiloba samples from the Seoul, Daegu, and Cheongju herbal medicinal markets. Fifty sections of one collection were mixed and ground to make a powder, which was used for DNA extraction using the cetyl trimethylammonium bromide (CTAB) method. Chloroplast based SSR markers were applied to the DNA for the determination of the species. In addition, polymorphism was found in eight samples. The phylogenetic analysis showed that the A. gigas roots collected from herbal medicinal markets were clearly discriminated from A. sinensis and A. acutiloba even though they were grouped into four clusters.
Conclusions: This study showed that chloroplast based SSR markers would help the discrimination of Angelica roots in the Korean herbal medicinal industry and the markers are useful to prevent confusion between Angelica roots.

KCI등재

3지방분화가 유도된 3T3-L1 세포와 고지방식이로 유도된 마우스에서 보리순 물추출물의 항비만 효과

저자 : 강병만 ( Byoung Man Kang ) , 심미옥 ( Mi Ok Sim ) , 김민석 ( Min Suk Kim ) , 유승진 ( Seung Jin Yoo ) , 여준환 ( Jun Hwan Yeo ) , 정원석 ( Won Seok Jung )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 367-374 (8 pages)

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Background: An imbalance in energy intake and expenditure can cause obesity, which is a major risk factor for chronic diseases such as heart disease, type 2 diabetes, insulin resistance, cancers and hyperlipidemia.
Methods and Results: In this study, we evaluated the anti-obesity effects of a water extract from the young leaves of barley sprout (BS) in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice (HF). Lipid accumulation measurement indicates that BS markedly inhibited adipogenesis by reducing lipid droplet production in a dose-dependent manner. Furthermore, the mRNA expression of adipogenic transcription factors peroxisome proliferator-activated receptor-γ and fatty acid synthetase, CCAAT/enhancer binding protein-α and fatty acid binding protein 4 in 3T3-L1 cells was significantly inhibited by BS treatment. In an in vivo test, the BSadministered group of HFD-induced mice showed less body weight gain, and lower liver and epididymal white adipose tissue weights. The BS-treated mice showed decreased serum levels of leptin and lipids compared to untreated HFD mice and the levels of adiponectin and the HDL-cholesterol/total cholesterol ratio increased. These results indicate that BS inhibits body fat accumulation by reducing the mRNA expression of lipogenesis transcription factors and increasing serum adipokine concentration in in vitro and in vivo tests.
Conclusions: BS reduced high fat diet-induced weight gain and had a positive effect on dyslipidemia.

KCI등재

4참소리쟁이 뿌리 추출물 및 분획의 항균 활성과 항생제 증강 활성

저자 : 양선아 ( Sun A Yang ) , 김아영 ( A Young Kim ) , 표병식 ( Byoung Sik Pyo ) , 김선민 ( Sun Min Kim )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 375-380 (6 pages)

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Background: The objective of this study were to determine the antibacterial activity and antibiotic activity-enhancing effect of 70% ethanol extract of the root of Rumex japonicus Houtt. and its fractions when used in combination with gentamicin against aerobic skin flora.
Methods and Results: The antibacterial activity and antibiotic (gentamicin) activity enhancing effect against aerobic skin flora were determined using the disc diffusion assay. Chloroform fraction (CF) and ethyl acetate fraction (EF) showed higher activities against Staphylococcus aureus and Staphylococcus epidermidis than those shown by other fractions. Regarding the antibiotic (gentamicin) activity-enhancing effect against aerobic skin flora, the n-hexane fraction (HF) and CF showed strong activity. The combination of HF and CF with gentamicin was evaluated using the broth dilution assay to determine the inhibitory effect on the growth of aerobic skin flora. The combination of CF with gentamicin exhibited the highest inhibitory effect on the growth of S. aureus and S. epdermidis. MTT assay performed to determine the viability of L929 cells revealed that EF treatment resulted in viability of 33.96 - 116.76% at the tested concentration. The combination of 70% ethanol extract and its other fractions with gentamicin showed low cell toxicity.
Conclusions: Appropriate use of antimicrobial agents is important prior to the development of new antibiotics. The 70% ethanol extract of the root of R. japonicus Houtt. and its fractions showed significant synergism with gentamicin when used in combination against S. aureus and S. epdermidis. Thus, R. japonicus Houtt. could be used as a functional materials in antimicrobial-related fields.

KCI등재

5한국과 중국 인삼자원들의 양적 형질 특성과 유전적 유연관계 분석

저자 : 송범헌 ( Beom Heon Song ) , 오문국 ( Wen Guo Wu ) , 김도현 ( Do Hyun Kim ) , 정종욱 ( Jong Wook Chung )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 381-388 (8 pages)

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Background: This study was conducted to acquire basic information on the phenotypic and genotypic characteristics of the germplasm of Panax ginseng C. A. Meyer collected from China and Korea, and identify the variations that can be utilized in ginseng breeding programs. Methods and Results: Quantitative parameters were evaluated, and used to compare and analyze on genetic polymorphisms in the germplasm. The genetic characteristics and classifications were compared and analyzed for each character. Stem length followed a normal frequency distribution ranging from 15.5 ㎝ to 40.5 ㎝, with showing approximately 40% having a stem length of 20 - 30 ㎜. Stem diameters ranged from 2.7 ㎜ to 11.3 ㎜. Stem number per plant ranged from 1 to 3; approximately 50% had a single stem, and 45% had two stems. A non-normal frequency distribution was observed for petiole number, with approximately 60% of the germplasm having 3 - 5 petioles. Petiole length exhibited a normal frequency distribution, raging from 4.5 to 10.6. Petiole angle in the germplasm ranged from 28° to 89° and seedstalk length ranged from 5.6 ㎝ to 27.3 ㎝. Conclusions: The genetic polymorphisms identified by complete linkage clustering based on the quantitative characteristics of Panax ginseng C. A. Meyer collected from Korea and China were classified to 6 groups, namely I, II, III, IV, V, and VI with frequencies of 6.7%, 20.0%, 31.7%, 8.3%, 6.7%, and 26.7%, respectively.

KCI등재

6SSR 마커를 이용한 산양삼의 유전적 다양성 분석

저자 : 길진수 ( Jinsu Gil ) , 엄유리 ( Yurry Um ) , 변재경 ( Jae Kyung Byun ) , 정종욱 ( Jong Wook Chung ) , 이이 ( Yi Lee ) , 정찬문 ( Chan Moon Chung )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 389-396 (8 pages)

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Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers.
Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background.
Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

KCI등재

7차광처리에 의한 삼채의 생육, 수량 및 당함량 변화

저자 : 김명희 ( Myung Hee Kim ) , 송병민 ( Beong Min Song ) , 최은영 ( Eun Young Choi )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 397-403 (7 pages)

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Background: This study was aimed at evaluating the growth, yield, and carbohydrate content in the whole Allium hookeri plant with shading treatment in hot summer.
Methods and Results: Different shading rate, including 0 (control), 35 or 55%, was employed from the June 21st to August 31st. Daily average air and soil temperature, which were approximately 2.5℃ and 3.8℃ lower, respectively, were observed with both 35% and 55% treatments in July and August, with no significant difference in daily maximum air temperature. Dry weights were high, approximately 40% and 48% for the shoot and 20% and 12% for the root, with the 35% and 55% treatments, respectively, 8 weeks after shading. Division number was increased by 13% and 19.8% with the 35% and 55% treatments, respectively. The mortality rates of 150 plants were 9.1%, 4.0%, and 1.3% with the 0 (control), 35% and 55% treatments, respectively. At 4 weeks after shading, the highest and lowest sucrose levels in both shoot and root were observed with the 35% and 55% treatments, respectively. At 8 weeks after shading, there was no significant difference in the sucrose content in the shoot among the treatments.
Conclusions: The highest plant growth rate and yield with the 55% treatment may be related with the decrease in both air and soil temperatures, resulting in reducted leaf respiration and thus compensate net photosynthesis.

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8금잔화, 상황, 어성초 에탄올 추출물의 세포독성 평가 및 3T3-L1 세포에 대한 육모 효과

저자 : 진성우 ( Seong Woo Jin ) , 고영우 ( Young Woo Koh ) , 윤경원 ( Kyeong Won Yun ) , 김경제 ( Kyung Je Kim ) , 제해신 ( Hae Shin Je ) , 임승빈 ( Seung Bin Im ) , 김광상 ( Kwang Sang Kim ) , 김민숙 ( Min Sook Kim ) , 유병조 ( Byung Jo Yu ) , 서경순 ( Kyoung Sun Seo )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 404-410 (7 pages)

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Background: Hair loss related syndromes are increasing due to environmental pollution and stress. Hair care products are mainly prepared by mixing chemicals and natural extracts, such as those obtained from medicinal plants. The purpose of this study was to investigate the effects of 70% ethanol extracts from the flowers of Calendula officinalis, fruit body of Phellinus linteus, and the whole plant of Houttuynia cordata on the growth of CCD-986 cells, hair follicle dermal papilla cells (HFDPC), and 3T3-L1 cells.
Methods and Results: All sample extracts at all concentrations, except for that from P. linteus fruit body at 500㎍/㎖, were cytotoxic to CCD-986 cells. However, none of the sample extracts were cytotoxic to HFDPC. The lipid differentiation of 3T3-L1 cells regulates hair regeneration via secretion of platelet derived growth factor. The 70% ethanol extract of H. cordata whole plant promoted hair growth. Adipogenesis rate significantly increased in a treatment concentration-dependent manner.
Conclusions: These results suggest that 70% ethanol extracts of C. officinalis flower, P. linteus fruit body and H. cordata could be used for the development of hair care products.

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9차세대염기서열분석법을 이용한 잔대의 SSR 마커 개발

저자 : 박기찬 ( Ki Chan Park ) , 김영국 ( Young Guk Kim ) , 황보경 ( Kyeong Hwangbo ) , 길진수 ( Jinsu Gil ) , 정희 ( Hee Chung ) , 박신기 ( Sin Gi Park ) , 홍창표 ( Chang Pyo Hong ) , 이이 ( Yi Lee )

발행기관 : 한국약용작물학회 간행물 : 한국약용작물학회지 25권 6호 발행 연도 : 2017 페이지 : pp. 411-417 (7 pages)

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Background: Adenophora triphylla var. japonica (Regel) H. Hara shows vegetative growth with radical leaves during the first year and shows reproductive growth with cauline leaves and bolting during the second year. In addition, the shape of the plant varies within the same species. For this reason, there are limitations to classifying the species by visual examination. However, there is not sufficient genetic information or molecular tools to analyze the genetic diversity of the plant.
Methods and Results: Approximately 34.59 Gbp of raw data containing 342,487,502 reads was obtained from next generation sequencing (NGS) and these reads were assembled into 357,211 scaffolds. A total of 84,106 simple sequence repeat (SSR) regions were identified and 14,133 primer sets were designed. From the designed primer sets, 95 were randomly selected and were applied to the genomic DNA which was extracted from five plants and pooled. Thirty-nine primer sets showing more than two bands were finally selected as SSR markers, and were used for the genetic relationship analysis.
Conclusions: The 39 novel SSR markers developed in this study could be used for the genetic diversity analysis, variety identification, new variety development and molecular breeding of A. triphylla.

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