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한국환경농학회지 update

KOREAN JOURNAL OF ENVIRONMENTAL AGRICULTURE

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수록정보
수록범위 : 1권1호(1982)~39권1호(2020) |수록논문 수 : 1,610
한국환경농학회지
39권1호(2020년 03월) 수록논문
최근 권호 논문
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1일천궁의 연작재배에 따른 토양 이화학성 및 토양세균군집 연구

저자 : 김기윤 ( Kiyoon Kim ) , 한경민 ( Kyeung Min Han ) , 김현준 ( Hyun-jun Kim ) , 전권석 ( Kwon Seok Jeon ) , 김충우 ( Chung Woo Kim ) , 정충렬 ( Chung Ryul Jung )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 1-9 (9 pages)

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본 연구에서는 일천궁의 연작재배에 따른 토양 이화학성 및 토양세균군집을 비교 분석하기 위해 천궁 중에서 재배 빈도수가 높은 일천궁의 초작 및 연작 재배지를 선정하여 토양 이화학성 및 토양세균군집 특성을 분석하고, 토양 이화학성 및 토양세균군집 간의 상관관계를 구명하고자 하였다. 토양 이화학성은 농촌진흥청 토양분석법을 이용하였고, 토양세균군집 분석은 Illumina Miseq sequencing system을 이용하여 상대적 빈도수 및 주좌표 분석을 하였다. 토양 이화학성과 토양세균군집 간의 상관관계는 DISTLM과 Spearman's 상관관계 분석을 이용하였다. 일천궁 재배지의 토양세균군집은 일천의 재배법에 따라 뚜렷하게 2개의 cluster로 군집화를 이루었고, 초작 및 연작 재배지 모두 Proteobacteria 와 Alphaproteobacteria가 우점종으로 나타났다. 주좌표 분석과 DISTLM 분석에서는 일천궁 재배지의 토양 pH와 Ca이 토양세균의 군집화에 유의적으로 영향을 주고 있음을 확인하였고, Spearman's 상관관계 분석을 통해 일천궁의 재배법에 따라 유의적인 차이를 보였던 Acinobacteria 와 Acidobacteria 의 상대적 빈도수는 토양 pH, Ca 함량과 유의적인 상관관계를 보이는 것으로 나타났다. 본 연구는 일천궁의 재배법에 따른 토양 이화학성 및 토양세균군집의 변화와 상관관계를 구명하는데 중요한 자료가 될 것으로 판단된다. 또한, 토양 이화학성과 토양세균군집의 변화에 따른 일천궁 재배지의 연작장해 원인을 구명하는데 있어 도움을 줄 수 있을 것이다. 향후 본 연구를 바탕으로 향후 일천궁의 재배법에 따른 곰팡이(fungi)의 군집과 병원성 미생물군집의 변화를 확인하고 토양 이화학성과의 상관 관계를 분석할 수 있다면 일천궁의 연작장해 원인을 명확하게 구명하는데 도움을 줄 수 있을 것으로 사료된다.


BACKGROUND: The aim of this study was to investigate the soil chemical properties and soil bacterial community of the cropping system for Cnidium officinale Makino.
METHODS AND RESULTS: The bacterial community was analyzed for the relative abundance and principal coordinated analysis (PCoA analysis) by using by Illumina Miseq sequencing. The correlation analysis between soil chemical properties and soil bacterial community were analyzed by Spearman's rank correlation and DISTLM analysis. Soil bacterial community (phylum and class) showed two distinct clusters consisting of cluster 1 (first cropping) and cluster 2 (continuous cropping) from 2 different cultivation methods of Cnidium officinale Makino. PCoA and DISTLM analyses showed that soil pH and Ca significantly affected soil bacterial community in cultivation area of Cnidium officinale Makino. In addition, Spearman's rank correlation showed significant correlation between relative abundance (Acidobacteria and Actinobacteria) and soil factors (soil pH and Ca).
CONCLUSION: The results of this study were considered to be important for determining the correlation between soil properties and soil bacterial community of the cropping method for Cnidium officinale Makino. Furthermore, the results will be helpful to investigate the cause of continuous cropping injury of the Cnidium officinale Makino by examining the changes of soil properties and soil bacterial communities.

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2폐광산 인근 지역에서 생산되는 농산물의 중금속 오염도 평가

저자 : 안재민 ( Jae-min An ) , 장순영 ( Soon-young Chang ) , 황향란 ( Hyang-ran Hwang ) , 박대한 ( Dae-han Park ) , 이봄내 ( Bom-nae Lee ) , 김샛별 ( Saet-byeol Kim ) , 이광희 ( Gwang-hee Lee )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 10-19 (10 pages)

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본 연구에서는 대구·경북지역 36개 폐광산 인근지역에서 생산되는 25개 농산물 1,059건에 대하여 카드뮴, 납, 비소 오염도 조사와 더불어 위험평가를 실시하였다. 정량한계는 카드뮴 0.59 μg/kg, 납 0.42 μg/kg, 총비소 0.55 μg/kg, 무기비소 화학종에서는 arsenite [(As(Ⅲ)) 1.74 μg/kg], arsenate [(As(Ⅴ)) 2.25 μg/kg]이었으며, 직선성은 모두 0.9995 이상으로 나타났고, 무기비소의 함량은 arsenite (As(Ⅲ))와 arsenate (As(Ⅴ))의 합으로 하였다. 평균 회수율은 카드뮴 96.3-97.7%, 납 95.8-98.2%, 총비소 96.1-98.0%, arsenite 94.8%, arsenate 115.4% 이었고, C.V.% (Coefficient of Varidation)는 모두 5% 미만으로 나타났다. 농산물의 중금속 평균 함량은 도라지 0.244±0.164 mg/kg, 대파 0.145±0.133 mg/kg, 참깨 0.124±0.136 mg/kg, 고사리 0.109±0.070 mg/kg, 취나물 0.103±0.093 mg/kg, 마늘 0.071±0.063 mg/kg의 순으로 카드뮴의 오염도가 높았고, 납은 참깨 0.040±0.085 mg/kg, 도라지 0.039±0.056 mg/kg, 고구마 0.034±0.058 mg/kg, 들깻잎 0.034±0.016 mg/kg, 호박 0.032±0.065 mg/kg, 매실 0.028±0.012 mg/kg의 순으로 높은 오염도를 보였다. 무기비소는 쌀에 한하여 조사되었는데 평균 0.082±0.035 mg/kg의 오염도를 보였다. 카드뮴의 경우 쌀 1.19×10-1 μg/kg b.w./day (14.37% of PTMI), 대파 2.77×10-2 μg/kg b.w./day (3.34% of PTMI), 감자 2.08×10-2 μg/kg b.w./day (2.51% of PTMI), 사과 1.39×10-2 μg/kg b.w./day (1.67% of PTMI), 양파 1.13×10-2 μg/kg b.w./day (1.37% of PTMI)의 순이었고, 납의 경우 사과 1.29×10-2 μg/kg b.w./day (2.05% of PTWI), 고구마 7.49×10-3 μg/kg b.w./day (1.19% of PTWI), 쌀 7.15×10-3 μg/kg b.w./day (1.14% of PTWI), 호박 5.01×10-3 μg/kg b.w./day (0.79% of PTWI), 양파 4.80×10-3 μg/kg b.w./day (0.76% of PTWI)의 순으로 납 노출량이 높았다. 본 연구의 조사대상 25개 품목 농산물을 모두 일시에 섭취한다고 가정하였을 경우, 카드뮴은 PTMI 대비 28.21%, 납은 PTWI 대비 7.94%, 무기비소는 쌀 단일품목에 한해 PTWI 대비 15.16%의 위해도를 보임으로써, 본 연구에서 진행하였던 중금속에 대한 인체 위해정도는 우려할 수준이 아닌 것으로 확인되었다.


BACKGROUND: This study was to carry out risk assessment of contamination of cadmium (Cd), lead (Pb), and inorganic arsenic (I-As) in agricultural products of 25 crops from the abandoned mine areas. The 36 typical investigation sites located in Gyeongbuk provincial area were selected by considering the heavy metal levels, that had been known that the amount of the heavy metals exceeded the contamination level based on the previous survey.
METHODS AND RESULTS: Cadmium, lead, and total arsenic (T-As) concentrations were determined using microwave device and ICP-MS. Inorganic arsenic was determined by HPLC-ICP-MS. The limits of quantification for heavy metals were 0.59 μg/kg for Cd, 0.42 μg/kg for Pb, 0.55 μg/kg for T-As, and sum of As (Ⅲ) (1.74 μg/kg) and As (Ⅴ) (2.25 μg/kg) for I-As, respectively. The contents of Cd, Pb, and I-As (only rice) were N.D.-0.958 mg/kg, N.D.-0.227 mg/kg, and 0.082 mg/kg, respectively, in the agricultural products. For risk assessment, dietary exposures of heavy metals through usual intake were 5.20×10-4-7.15×100 μg/day for Cd, 7.00×10-5-7.75×10-1 μg/day for Pb, and 1.17×101 μg/day for I-As, taking 0.01-14.37%, 0.01-2.05%, and 15.16% as risk indices, respectively.
CONCLUSION: It requires to consider the critical levels of heavy metals in agricultural products due to unexpectedly high levels in a few places, while concentrations of heavy metals in the samples were relatively low in most areas.

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3후코이단과 후코이단 이용 박테리아의 멜론 성장 촉진 효과 검증

저자 : 양소희 ( Sohee Yang ) , 길예지 ( Yeji Gil ) , 오희정 ( Heejeong Oh ) , 구연종 ( Yeonjong Koo )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 20-25 (6 pages)

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BACKGROUND: Marine algae is a productive organism that is consumed as a nutritious food. However, large amounts of unused portions of the algae are incinerated as trash or dumped in the sea, causing pollution. Recycling algae is important for saving resources and conserving the environment. In this study, the fucoidan which is a major carbohydrate of marine algae was tested as a source of fertilizer for farming.
METHODS AND RESULTS: The growth rate of the melon was examined after treating fucoidan and the melon growth factors, weight and length of stem were measured. To discover the mechanism of melon growth promotion of fucoidan, bacteria that decomposed fucoidan were isolated from soil and abalone. Bacillus wiedmannii and Stenotrophomonas pavanii were isolated from terrestrial soil and Pseudomonas sp. was isolated from abalone. Among these three bacteria, Pseudomonas sp. had the highest and most specific fucoidan-decomposing activity. When Pseudomonas sp. was treated with fucoidan on melon-growing soil, the growth of melon was relatively improved compared to the treatment with fucoidan alone.
CONCLUSION: We found that fucoidan, the main carbohydrate of marine algae, promoted melon growth. Fucoidan-decomposing microorganisms were isolated from terrestrial soil and marine organism, and we found that these bacteria stimulated the effect of melon growth promotion of marine algae. This is the first report that confirms the fertilizer effect of marine algae and shows the use of bacteria with marine algae.

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4Detection and Potential Abundances of Anammox Bacteria in the Paddy Soil

저자 : Anamika Khanal , Seul Lee , Ji-hoon Lee

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 26-35 (10 pages)

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BACKGROUND: Microbes that govern a unique biochemical process of oxidizing ammonia into dinitrogen gas, such as anaerobic ammonium oxidation (anammox) have been reported to play a pivotal role in agricultural soils and in oceanic environments. However, limited information for anammox bacterial abundance and distribution in the terrestrial habitats has been known.
METHODS AND RESULTS: Phylogenetic and next-generation sequencing analyses of bacterial 16S rRNA gene were performed to examine potential anammox bacteria in paddy soils. Through clone libraries constructed by using the anammox bacteria-specific primers, some clones showed sequence similarities with Planctomycetes (87% to 99%) and anammox bacteria (94% to 95%). Microbial community analysis for the paddy soils by using Illumina Miseq sequencing of 16S rRNA gene at phylum level was dominated by unclassified Bacteria at 33.2 ± 7.6%, followed by Chloroflexi at 20.4 ± 2.0% and Acidobacteria at 17.0 ± 6.5%. Planctomycetes that anammox bacteria are belonged to was 1.5% (± 0.3) on average from the two paddy soils.
CONCLUSION: We suggest evidence of anammox bacteria in the paddy soil. In addition to the relatively well-known microbial processes for nitrogen-cycle, anammox can be a potential contributor on the cycle in terrestrial environments such as paddy soils.

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5경상북도 위천수계의 수리화학적 특성 및 관개용수 수질평가

저자 : 이기창 ( Gi-chang Lee ) , 박명섭 ( Moung-sub Park ) , 김재식 ( Jae-sik Kim ) , 장태권 ( Tae-kwon Jang ) , 김효순 ( Hyo-sun Kim ) , 이화성 ( Hwa-sung Lee ) , 손진창 ( Jin-chang Son )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 36-43 (8 pages)

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본 연구는 낙동강수계 중권역 중 최대의 농업용수 관개면적을 가지는 위천수계를 대상으로 관개용수에 대한 기초자료 마련을 위해 수리화학적 특성과 관개용 수질특성을 평가하였다. 위천수계의 유기물 오염도와 염분 함량을 나타내는 평균 BOD5 농도와 EC는 각각 1.1 mg/L, 350 μS/cm로 좋은 수질을 보였고, 이 중 남천은 BOD5 0.5 mg/L, EC 157 μS/cm로 가장 낮아 매우 깨끗한 수질을 나타내었다. 위천은 각 지류와 하수처리방류수가 유입되는 쌍계천의 합류로 인해 하류로 갈수록 염분 농도가 미미하게 증가하였으나 영향은 크지 않았다. 금속류에 대해서도 매우 안전한 수질을 보여 토양과 농산물 재배에 미치는 유해성은 없는 것으로 나타났다. 위천수계의 미네랄 형성은 탄산염암 풍화로 인한 물-암석 교환작용의 영향이 우세한 것으로 나타났고, Ca-HCO3-Cl 수질유형에 집중분포 하였다. 위천수계의 양이온과 음이온에 대한 분포농도는 각각 Ca2+>Mg2+>Na+>K+, HCO3->SO42->Cl-> F- 순으로 존재하였다. 이들 성분에 대해 남천은 다른 하천에 비해 2배 이상 낮은 농도분포를 보였다. 구천의 경우 Ca2+과 SO42- 농도는 다른 하천보다 각각 1.2-3.2배, 1.6-3.1배 높게 형성하고 있었다. SAR, %Na, PI, RSC 지수를 평가한 결과 위천수계 수질은 염분의 영향이 매우 낮아 농산물 재배에 적합한 관개용수로 나타났다. 특히 염분농도는 벼 수확량에 미치는 영향이 크므로 위천수계의 대표 농작물인 벼 재배를 위한 매우 중요한 수질인자이다. 위천수계는 FAO와 국내 연구자료에서 제시한 벼 재배에 대한 염분 허용농도기준을 충분히 만족하였고, 다른 농작물 생산에도 전혀 피해가 없는 우수한 수질을 갖는 것으로 나타났다. 앞으로 본 연구결과가 농산물 재배 및 우수성 홍보 등에 기초자료로 활용되어 지역 농업활동에 도움이 되길 기대한다.


BACKGROUND: Wicheon watershed has the largest irrigation area among the mid-watershed of Nakdong river. However, no investigation of irrigation water quality has been conducted on the Wicheon watershed, which evaluates the effects on the soil quality and crop cultivation. Therefore, this study aims to provide various assessments of water quality of Wicheon watershed as the scientific basic data for efficient agricultural activities.
METHODS AND RESULTS: Water sampling was performed in five locations of the first tributaries of Wicheon. Wicheon watershed showed clean water quality with very low organic matters and safe water quality from metals at all points of investigation. It was estimated that the natural chemical components of Wicheon watershed were originated from water-rock interaction in Gibbs diagram. All samples were concentrated in the type of Ca-HCO3-Cl in the Piper diagram. The quality of irrigation water was evaluated with sodium adsorption ratio (SAR), residual sodium carbonate (RSC), permeability index (PI), and percent sodium (%Na). The values of these water quality indices were in the range of 0.37-0.67, -2.11--0.24, 41.13-84.52% and 11.28-21.84%, respectively, and were classified as good grades at all sites.
CONCLUSION: The water quality of Wicheon watershed was very low in salt, indicating good irrigation water suitable for growing agricultural products. We hope that the results of this study will be used as the basic data for the cultivation of agricultural products and promotion of their excellence.

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6농산물 및 경작지 토양 시료 중 Benzo(a)pyrene 신속잔류분석법 개선 연구

저자 : 김희곤 ( Hee-gon Kim ) , 함헌주 ( Hun-ju Ham ) , 홍경숙 ( Kyong-suk Hong ) , 신희창 ( Hee-chang Shin ) , 허장현 ( Jang Hyun Hur )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 44-49 (6 pages)

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BACKGROUND: Benzo(a)pyrene is a highly toxic substance which has been listed as a Group I carcinogen by the International Agency for Research on Cancer. There have been numerous studies by researchers worldwide on benzo(a)pyrene. Soxhlet, ultrasound-assisted, and liquid-liquid extractions have been widely used for the analysis of benzo(a)pyrene. However these extraction methods have significant drawbacks, such as long extraction time and large amount of solvent usage. To overcome these disadvantages, we aimed to establish a rapid residual analysis of benzo(a)pyrene content in agricultural products and soil samples.
METHODS AND RESULTS: A Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method was used as the pretreatment procedure. For rapid residual analysis of benzo(a)pyrene, a modified QuEChERS method were used, and the best codition was demonstrated after various performing instrument analysis. The extraction efficiency of this method was also compared with Soxhlet extraction, the current benzo(a)pyrene extracting method. Although both methods showed high recovery rates, the rapid residual analysis method markedly reduced both the measurement time and solvent usage by approximately 97% and 96%, respectively.
CONCLUSION: Based on these results, we suggest the rapid residual analysis method established through this study, faster and more efficient analysis of residual benzo(a)pyrene in major agricultural products such as rice, green and red chili peppers and also soil samples.

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7바이오차의 시용이 채소 유묘 생장 및 양분 흡수량에 미치는 영향

저자 : 홍성창 ( Sung-chang Hong ) , 유선영 ( Seon-young Yu ) , 김경식 ( Kyeong-sik Kim ) , 이규현 ( Gyu-hyun Lee ) , 송새눈 ( Sae-nun Song )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 50-57 (8 pages)

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BACKGROUND: Biochar is used in various environmental fields, such as water quality and soil restoration, and affects soil fertility and nutrient cycling. Also, when crops are grown on biochar-applied soil, their characteristics may be affected. Biochar is used especially with commercial vegetable seedlings.
METHODS AND RESULTS: The objective of this study was to determine the effects of biochar content in seeding mixes on early growth of lettuce (Lactuca sativa L.), Chinese cabbage (Brassica rapa L.), and red pepper (Capsicum annuum L.). Treatments consisted of a control (0: 10, ratio of biochar to seeding mixes (w/w)), 1: 9 (biochar 10%), 3: 7 (biochar 30%), 5: 5 (biochar 50%), and 7: 3 (biochar 70%). The biochar was made from risk husk and had a C/N ratio of 104. As the mixing ratio of biochar increased, pH increased whereas EC and nitrogen content decreased. The highest phosphorus content was with the treatment of 30% biochar, while there were significant increases in the weight of lettuce seedlings and concentrations of T-N, P2O5, K2O, MgO, and Na with the treatments of 30% and 50% biochar. Although the weight of Chinese cabbage seedlings increased with the treatment of 10% biochar, the increase was not statistically significant. Also, there was an increase in the weight of red pepper seedlings with the treatment of 30% biochar, but the increase was not statistically significant. With increases in the biochar mixing ratio, the K2O concentration of red pepper seedlings increased, but the concentrations of P2O5, CaO, MgO, and Na decreased. It was believed that this was because of absorption inhibition by calcium-phosphate formation in the seeding mixes owing to increased pH.
CONCLUSION: In conclusion, adding biochar to seeding mixes is considered to be an important mean for growing healthy vegetable seedlings. More field experiments are needed to verify the effect of biochar on vegetable crop growth over the entire growing season.

KCI등재

8고도산화공정인 오존처리에 의한 난분해성 염료 수용액의 분해특성

저자 : 황세욱 ( Se-wook Hwang ) , 박종환 ( Jong-hwan Park ) , 이수림 ( Su-lim Lee ) , 엄주현 ( Ju-hyun Eom ) , 류성기 ( Sung-ki Ryu ) , 최익원 ( Ik-won Choi ) , 김성헌 ( Seong-heon Kim ) , 강세원 ( Se-won Kang ) , 조주식 ( Ju-sik Cho ) , 서동철 ( Dong-cheol Seo )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 58-64 (7 pages)

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BACKGROUND: Most of the researches on the dye removal using ozonation have been focused on the removal efficiency. However, the research on their removal characteristics and mechanism according to the reaction time has been still insufficient.
METHODS AND RESULTS: In this study, the effects of initial pH and dye concentration with reaction time on the degradation characteristics of methyl orange (MO) and methylene blue (MB) by ozonation were evaluated. The degradation efficiency of MB by ozonation increased with increasing pH. On the other hand, the degradation efficiency of MO by ozonation did not show a significant difference with varing pH. The both MO and MB by ozonation were decomposed within 30 min irrespective of the dye concentration, but the decomposition rates of dyes were faster at lower initial dye concentration. The decomposition efficiency of total organic carbon (TOC) in each dye solution by ozonation was low, which was found to be effective for partial decomposition such as decolorization rather than complete degradation of the dye.
CONCLUSION: Overall, ozonation was an effective method for removing nondegradable dyes. However, it is necessary to study the optimization of dye degradation under various environmental conditions for ozonation.

KCI등재

9유기농 옥수수밭에서 경운이 토양 유기물 함량 및 미생물군집에 미치는 영향

저자 : 안달래 ( Dalrae Ahn ) , 안난희 ( Nan-hee An ) , 김다혜 ( Da-hye Kim ) , 한병학 ( Byeong-hak Han ) , 유재홍 ( Jaehong You ) , 박인철 ( Incheol Park ) , 안재형 ( Jae-hyung Ahn )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 65-74 (10 pages)

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BACKGROUND: Soil carbon sequestration has been investigated for a long time because of its potential to mitigate the greenhouse effect. No- or reduced tillage, crop rotations, or cover crops have been investigated and practiced to sequester carbon in soils but the roles of soil biota, particularly microorganisms, have been mostly ignored although they affect the amount and stability of soil organic matters.
METHODS AND RESULTS: In this study we analyzed the organic matter and microbial community in organically cultivated corn field soils where no-tillage (NT) or conventional tillage (CT) had been practiced for about three years. The amounts of organic matter and recalcitrant carbon pool were 18.3 g/kg dry soil and 4.1 g C/kg dry soil, respectively in NT soils, while they were 12.4 and 2.5, respectively in CT soils. The amounts of RNA and DNA, and the copy numbers of bacterial 16S rRNA genes and fungal ITS sequences were higher in NT soils than in CT soils. No-tillage treatment increased the diversities of soil bacterial and fungal communities and clearly shifted the bacterial and fungal community structures. In NT soils the relative abundances of bacterial phyla known as copiotrophs, Betaproteobacteria and Bacteroidetes, increased while those known as oligotrophs, Acidobacteria and Verrucomicrobia, decreased compared to CT soils. The relative abundance of a fungal phylum, Glomeromycota, whose members are known as arbuscular mycorrhizal fungi, was about two time higher in NT soils than in CT soils, suggesting that the higher amount of organic matter in NT soils is related to its abundance.
CONCLUSION: This study shows that no-tillage treatment greatly affects soil microbial abundance and community structure, which may affect the amount and stability of soil organic matter.

KCI등재

10시설재배 브로콜리 중 Indoxacarb 및 Pymetrozine의 잔류 소실특성

저자 : 양승현 ( Seung-hyun Yang ) , 이재인 ( Jae-in Lee ) , 최훈 ( Hoon Choi )

발행기관 : 한국환경농학회 간행물 : 한국환경농학회지 39권 1호 발행 연도 : 2020 페이지 : pp. 75-82 (8 pages)

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본 연구에서는 시설재배 하는 브로콜리 중 indoxacarb 및 pymetrozine의 경시적 잔류변화를 조사하여 생물학적 반감기와 감소상수를 산출하여 생산단계 잔류허용기준(PHRL)을 설정하고자 하였다. 포장시험은 충주시(포장 1) 및 군산시(포장 2)에 소재한 시설재배지에서 수행되었으며 약제살포 후 0, 1, 2, 3, 5, 7, 10일차에 시료를 채취하여 분석하였다. 브로콜리 중 잔류량은 HPLC-DAD로 분석하였으며, indoxacarb 및 pymetrozine의 회수율은 각각 94.3~105.4% 및 81.8~96.0% 이었으며, MLOQ (Method Limit of quantitation)는 모두 0.05 mg/kg이었다. 브로콜리 중 indoxacarb 및 pymetrozine의 생물학적 반감기는 각각 2.9일, 3.2~3.8일이었으며, 감소상수의 95% 신뢰수준 하한치는 indoxacarb 0.1508 및 0.2017, pymetrozine 0.1489 및 0.1577로써 포장별, 약제별 유의적 차이(p<0.05)가 없었다. 브로콜리 중 농약 잔류량 소실특성의 주요 요인은 증체로 인한 희석효과이었으며, 브로콜리의 수확 10일전 PHRL은 indoxacarb의 경우 30.06(포장 1) 및 18.07(포장 2) mg/kg이었고, pymetrozine은 4.84(포장 1) 및 4.43(포장 2) mg/kg이었다.


BACKGROUND: This study was carried out to establish pre-harvest residue limits (PHRLs) of indoxacarb and pymetrozine in broccoli under greenhouse conditions, based on dissipation patterns and biological half-lives of pesticides during 10 days after application.
METHODS AND RESULTS: The field studies were conducted in two different greenhouse, located in Chungju-si (Field 1) and Gunsan-si (Field 2). Samples were collected at 0, 1, 2, 3, 5, 7 and 10 days after spraying pesticide suspension. The analytical methods for indoxacarb and pymetrozine using HPLC-DAD were validated by recoveries ranging of 94.3-105.4% and 81.8-96.0%, respectively, and MLOQ (Method Limit of Quantification) of 0.05 mg/kg. Biological half-lives of indoxacarb and pymetrozine were 2.9 and 3.2-3.8 days in broccoli, respectively. The lower 95% confidence intervals of dissipation rate constant of indoxacarb were determined as 0.1508 (Field 1) and 0.2017 (Field 2), whereas those of pymetrozine were calculated as 0.1489 (Field 1) and 0.1577 (Field 2).
CONCLUSION: The significant differences were not observed between the dissipation rates of indoxacarb and pymetrozine in broccoli. The major factor affecting residue dissipation was the dilution effect by fast growth. The PHRLs for 10 days prior to harvest were recommended as 30.06 (Field 1) and 18.07 (Field 2) mg/kg for indoxacarb, and 4.84 (Field 1) and 4.43 (Field 2) mg/kg for pymetrozine, respectively.

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(2006)홍길동 외 1명심리학41회 피인용

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(2006)홍길동41회 피인용

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